Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   B4X03_RS00420 Genome accession   NZ_CP020085
Coordinates   64134..66347 (-) Length   737 a.a.
NCBI ID   WP_082259017.1    Uniprot ID   -
Organism   Glaesserella parasuis strain CL120103     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 59134..71347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B4X03_RS00400 - 59539..60441 (-) 903 WP_021114484.1 DMT family transporter -
  B4X03_RS00405 - 60584..61681 (+) 1098 WP_082259011.1 ISAs1 family transposase -
  B4X03_RS00410 - 61894..62713 (-) 820 Protein_75 transposase -
  B4X03_RS00415 thiB 63111..64094 (+) 984 WP_021111382.1 thiamine ABC transporter substrate binding subunit -
  B4X03_RS00420 priA 64134..66347 (-) 2214 WP_082259017.1 primosomal protein N' Machinery gene
  B4X03_RS00425 rarD 66347..67213 (-) 867 WP_005711008.1 EamA family transporter RarD -
  B4X03_RS00430 rarD 67213..68094 (-) 882 WP_075631039.1 EamA family transporter RarD -
  B4X03_RS00435 - 68217..69434 (-) 1218 WP_005711010.1 pyridoxal phosphate-dependent aminotransferase -
  B4X03_RS00440 - 69581..70855 (+) 1275 WP_075621084.1 isochorismate synthase -

Sequence

Protein


Download         Length: 737 a.a.        Molecular weight: 83255.51 Da        Isoelectric Point: 8.7560

>NTDB_id=184643 B4X03_RS00420 WP_082259017.1 64134..66347(-) (priA) [Glaesserella parasuis strain CL120103]
MNLIQIALPVPLARYFDYLLPTHFENVVKGARVVVPFGRQRKIGIVVDFPTTSDIPTEKLKPIEQLLDEQALFDTEMWQL
LQWASRYYHAPFGEVLQSALPVKLRQGESSERTAPDYFVVTENGRKALSNGVVQKAKKQQELLSKLAEFANFFEKPTACS
PAVWKALFEKGYLEKLEIPFNVQSWQEKLGEQPICNTQNRLTLNKQQTLVVSRLHYQQGFGAFLLNGVTGSGKTEVYLQV
IEEVLKRGEQVLVLVPEIGLTPQTIQRFQARFNVEIDALHSHLNDTQRLNAWLRAKNGESAIVIGTRSALFTQFQQLGLI
IIDEEHDGSFKQQDGWRYHARDLAVLRAKNNNIPIILGSATPSFESLQNMQNGKFIELQLTARAGNAHFSKQTLIDLKKQ
RIYSGLSEKLLAMMQSHLEKGNQVMLFLNRRGFAPVLLCHECGWIADCPSCDKPFTYHQKQRVLRCHHCASQKAIPRQCG
HCGSVNLITTGIGTEQLEQVLAQRFPQYSITRIDRDSTARKGSLEQHLAEIQAGKSQILIGTQMLAKGHHFPNVTLVAIV
NVDSSLFSTDYRAEERLAQLYVQVSGRAGRAEKQGEVVLQTHYPDHPLLTSLLNDGYLAFASQALAMRKIMSLPPFSSQV
LIRATGRDSDKVANFLQDLTACLQQIIGENGWQGFQLLGPFNAPMAKKAGHYRWQLLIQHSARSAIQQLLDRFDEQKEQF
LRNDIRLTVDIDPQELG

Nucleotide


Download         Length: 2214 bp        

>NTDB_id=184643 B4X03_RS00420 WP_082259017.1 64134..66347(-) (priA) [Glaesserella parasuis strain CL120103]
ATGAACCTCATCCAAATTGCGCTACCCGTGCCGTTGGCACGTTATTTTGATTACCTGCTTCCAACGCATTTTGAAAATGT
GGTGAAAGGGGCTAGGGTCGTTGTGCCTTTTGGTCGCCAACGTAAAATCGGGATTGTGGTGGACTTTCCGACCACATCAG
ATATTCCCACCGAAAAACTGAAACCGATTGAGCAACTGTTAGATGAACAAGCTCTGTTTGACACTGAAATGTGGCAACTT
TTACAGTGGGCAAGTCGTTATTATCACGCCCCTTTCGGCGAAGTGCTACAAAGTGCTTTGCCTGTCAAACTACGACAAGG
GGAAAGTAGTGAACGCACTGCTCCCGACTATTTTGTTGTCACGGAAAACGGCAGAAAAGCTCTATCCAACGGCGTGGTTC
AAAAGGCAAAAAAACAACAAGAGCTATTAAGCAAACTGGCGGAATTTGCAAATTTCTTTGAAAAACCGACCGCTTGTAGC
CCTGCGGTATGGAAAGCTTTGTTTGAAAAAGGGTATCTGGAAAAGCTGGAAATCCCTTTTAACGTGCAAAGCTGGCAGGA
AAAATTAGGCGAACAGCCGATTTGCAACACTCAAAACCGTTTAACTTTGAATAAACAGCAGACCCTTGTCGTCAGCCGTT
TGCATTATCAACAAGGCTTCGGAGCATTTTTGCTCAATGGGGTCACTGGCTCAGGGAAAACGGAAGTCTATCTGCAAGTG
ATCGAAGAAGTGCTGAAACGGGGCGAGCAAGTGTTGGTGCTTGTGCCTGAAATCGGGCTGACCCCCCAAACCATTCAACG
TTTCCAAGCTCGTTTTAACGTGGAAATTGACGCATTGCATTCGCATCTTAACGACACTCAACGGCTCAATGCGTGGCTTC
GAGCAAAAAACGGCGAAAGTGCAATTGTGATCGGCACACGTTCCGCCTTGTTCACACAATTTCAGCAACTTGGCTTAATC
ATTATTGATGAAGAACACGATGGCTCATTCAAACAACAAGACGGCTGGCGTTATCACGCACGGGATTTGGCGGTGCTTCG
GGCGAAAAATAACAACATTCCGATCATTCTCGGCTCGGCAACGCCTAGTTTTGAAAGTTTGCAAAATATGCAAAACGGTA
AATTTATCGAACTTCAGCTTACTGCAAGGGCTGGTAATGCACACTTTTCCAAACAAACCTTAATTGATTTGAAAAAACAG
CGGATTTATTCGGGCTTATCGGAAAAGTTGTTGGCAATGATGCAAAGCCACTTGGAAAAAGGCAATCAAGTGATGTTGTT
TTTGAACCGACGGGGGTTTGCCCCTGTTTTACTTTGTCACGAATGTGGCTGGATTGCCGATTGCCCGTCCTGTGATAAGC
CCTTTACCTATCACCAAAAACAGCGAGTATTGCGTTGCCATCACTGTGCTTCACAAAAAGCCATTCCACGCCAGTGCGGA
CATTGTGGCTCAGTCAATTTGATTACCACAGGGATCGGCACAGAACAGCTGGAACAAGTGCTGGCACAGCGTTTTCCGCA
GTACAGTATTACTCGCATTGATCGTGACAGCACCGCTCGTAAAGGCTCGTTGGAACAACACCTTGCCGAAATCCAAGCAG
GCAAAAGCCAAATTTTGATCGGCACACAGATGTTAGCCAAAGGACACCATTTCCCGAATGTTACTTTAGTGGCAATCGTG
AATGTGGATAGTAGCCTGTTTTCCACTGATTACCGTGCAGAAGAACGTTTGGCACAGCTTTATGTACAAGTGTCAGGGCG
AGCAGGACGAGCGGAAAAGCAAGGGGAAGTGGTGCTACAAACGCATTACCCCGATCACCCGTTACTGACCAGCTTACTCA
ACGACGGCTACCTTGCCTTTGCAAGCCAAGCCTTAGCGATGCGAAAAATAATGAGCTTACCGCCGTTTTCTTCGCAAGTG
CTTATTCGTGCAACGGGTAGAGATAGCGACAAAGTTGCAAATTTTCTGCAAGATTTGACCGCTTGTTTACAACAAATTAT
CGGTGAAAATGGCTGGCAAGGCTTTCAGCTACTCGGACCTTTCAACGCTCCGATGGCAAAAAAAGCAGGGCATTATCGCT
GGCAGTTACTTATTCAACATTCGGCAAGAAGTGCGATCCAACAGTTGTTAGATCGTTTTGATGAGCAAAAAGAACAGTTC
CTGCGAAACGATATTCGATTGACGGTGGATATTGATCCGCAGGAGTTGGGGTAG

Domains

Predicted by InterProScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(5-101)

DEAD/DEAH box helicase (DEAD)

(221-366)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(448-473)

Primosomal protein N C-terminal domain (PriA_C)

(635-733)

Helicase conserved C-terminal domain (Helicase_C)

(497-591)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

45.027

100

0.455