Detailed information NTG0184585

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: A9E93_RS04575
• Genome accession: NZ_CP015953
• Coordinates: 1010734..1011798 (+)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila strain E6_N
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1005734..1016798

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A9E93_RS04565 (A9E93_04565)	-	1006774..1009158 (-)	2385	WP_027220940.1	penicillin-binding protein 1A	-
A9E93_RS04570 (A9E93_04570)	ravI	1009310..1010317 (-)	1008	WP_011946020.1	Dot/Icm T4SS effector RavI	-
A9E93_RS04575 (A9E93_04575)	pilM	1010734..1011798 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
A9E93_RS04580 (A9E93_04580)	pilN	1011802..1012350 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
A9E93_RS04585 (A9E93_04585)	pilO	1012364..1012963 (+)	600	WP_013101267.1	type 4a pilus biogenesis protein PilO	Machinery gene
A9E93_RS04590 (A9E93_04590)	pilP	1012960..1013544 (+)	585	WP_013101268.1	pilus assembly protein PilP	Machinery gene
A9E93_RS04595 (A9E93_04595)	pilQ	1013548..1015644 (+)	2097	WP_011946023.1	type IV pilus secretin PilQ	Machinery gene
A9E93_RS04600 (A9E93_04600)	aroK	1016097..1016618 (+)	522	WP_027219629.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=184585 A9E93_RS04575 WP_011215094.1 1010734..1011798(+) (pilM) [Legionella pneumophila strain E6_N]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=184585 A9E93_RS04575 WP_011215094.1 1010734..1011798(+) (pilM) [Legionella pneumophila strain E6_N]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAAGTTTACATCTAAAAATGTAGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAACTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAGCACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407