Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   AM448_RS10120 Genome accession   NZ_CP020055
Coordinates   1965031..1965699 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain AR_0069     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1960031..1970699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM448_RS10090 (AM448_10215) rfaQ 1960209..1961267 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  AM448_RS10095 (AM448_10220) waaA 1961680..1962957 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  AM448_RS10100 (AM448_10225) coaD 1962965..1963444 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  AM448_RS10105 (AM448_10230) mutM 1963483..1964292 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  AM448_RS10110 (AM448_10235) rpmG 1964390..1964557 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  AM448_RS10115 (AM448_10240) rpmB 1964578..1964814 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  AM448_RS10120 (AM448_10245) radC 1965031..1965699 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  AM448_RS10125 (AM448_10250) coaBC 1965871..1967091 (+) 1221 WP_000050158.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  AM448_RS10130 (AM448_10255) dut 1967069..1967527 (+) 459 WP_000976070.1 dUTP diphosphatase -
  AM448_RS10135 (AM448_10260) slmA 1967634..1968230 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  AM448_RS10140 (AM448_10265) - 1968276..1969235 (-) 960 WP_000340183.1 reverse transcriptase family protein -
  AM448_RS10145 (AM448_10270) pyrE 1969533..1970174 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=184340 AM448_RS10120 WP_001297375.1 1965031..1965699(-) (radC) [Escherichia coli strain AR_0069]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=184340 AM448_RS10120 WP_001297375.1 1965031..1965699(-) (radC) [Escherichia coli strain AR_0069]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGTTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGACTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment