Detailed information NTG0184322

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: AM448_RS05030
• Genome accession: NZ_CP020055
• Coordinates: 988488..989480 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain AR_0069
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 983488..994480

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AM448_RS05010 (AM448_05080)	otnK	984263..985525 (+)	1263	WP_000590392.1	3-oxo-tetronate kinase	-
AM448_RS05015 (AM448_05085)	ygbL	985522..986160 (+)	639	WP_001278994.1	aldolase	-
AM448_RS05020 (AM448_05090)	ygbM	986165..986941 (+)	777	WP_001136918.1	HPr family phosphocarrier protein	-
AM448_RS05025 (AM448_05095)	ygbN	987030..988394 (+)	1365	WP_000104456.1	GntP family transporter	-
AM448_RS05030 (AM448_05100)	rpoS	988488..989480 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
AM448_RS05035 (AM448_05105)	nlpD	989543..990637 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
AM448_RS05040 (AM448_05110)	pcm	990822..991448 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
AM448_RS05045 (AM448_05115)	surE	991442..992203 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
AM448_RS05050 (AM448_05120)	truD	992184..993233 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
AM448_RS05055 (AM448_05125)	ispF	993230..993709 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
AM448_RS05060 (AM448_05130)	ispD	993709..994419 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=184322 AM448_RS05030 WP_000081550.1 988488..989480(-) (rpoS) [Escherichia coli strain AR_0069]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=184322 AM448_RS05030 WP_000081550.1 988488..989480(-) (rpoS) [Escherichia coli strain AR_0069]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715