Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   A8142_RS16225 Genome accession   NZ_CP015911
Coordinates   3368765..3370150 (-) Length   461 a.a.
NCBI ID   WP_032876163.1    Uniprot ID   -
Organism   Bacillus velezensis strain LS69     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3363765..3375150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8142_RS16190 (A8142_16190) flgL 3363940..3364857 (-) 918 WP_003151396.1 flagellar hook-associated protein FlgL -
  A8142_RS16195 (A8142_16195) flgK 3364869..3366386 (-) 1518 WP_032876170.1 flagellar hook-associated protein FlgK -
  A8142_RS16200 (A8142_16200) - 3366402..3366884 (-) 483 WP_032876168.1 flagellar protein FlgN -
  A8142_RS16205 (A8142_16205) flgM 3366899..3367165 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  A8142_RS16210 (A8142_16210) - 3367235..3367654 (-) 420 WP_012118571.1 TIGR03826 family flagellar region protein -
  A8142_RS16215 (A8142_16215) comFC 3367728..3368417 (-) 690 WP_196770545.1 phosphoribosyltransferase family protein Machinery gene
  A8142_RS16220 (A8142_16220) - 3368423..3368707 (-) 285 WP_003151389.1 late competence development ComFB family protein -
  A8142_RS16225 (A8142_16225) comFA 3368765..3370150 (-) 1386 WP_032876163.1 DEAD/DEAH box helicase Machinery gene
  A8142_RS16230 (A8142_16230) - 3370259..3371107 (-) 849 WP_032876160.1 DegV family protein -
  A8142_RS16235 (A8142_16235) degU 3371205..3371894 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  A8142_RS16240 (A8142_16240) degS 3371971..3373134 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  A8142_RS16245 (A8142_16245) - 3373357..3374004 (+) 648 WP_007614107.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52474.86 Da        Isoelectric Point: 9.9925

>NTDB_id=183511 A8142_RS16225 WP_032876163.1 3368765..3370150(-) (comFA) [Bacillus velezensis strain LS69]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRINGFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWASFAKRQIT
YCRACVMMGRADELTSLYSWNQASENSWEPVKLSWEGTLTDGQKQAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVVIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
CQKLQLQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVIMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=183511 A8142_RS16225 WP_032876163.1 3368765..3370150(-) (comFA) [Bacillus velezensis strain LS69]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCAGCGCATCAACGGTTTTATAGAAAAAGAGTATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGTCCTTTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCTCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCAGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTCTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCGTGATAATAGATGAAGTAGATGCGTTTCCTTTTTCCGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACTGATTGGGTT
TGCCAAAAACTTCAATTACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCAGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCATCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATTGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(330-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.826

99.783

0.627
  comFA Latilactobacillus sakei subsp. sakei 23K

38.182

95.445

0.364

Multiple sequence alignment