Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   BV140_RS02220 Genome accession   NZ_CP020013
Coordinates   457208..459400 (-) Length   730 a.a.
NCBI ID   WP_005686778.1    Uniprot ID   Q19V35
Organism   Haemophilus influenzae strain 6P32H1     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452208..464400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BV140_RS02195 (BV140_450) relA 452502..454733 (+) 2232 WP_005686776.1 GTP diphosphokinase -
  BV140_RS02200 (BV140_451) - 454748..455104 (+) 357 WP_005632174.1 diacylglycerol kinase -
  BV140_RS02205 (BV140_452) mog 455187..455780 (+) 594 WP_005635167.1 molybdopterin adenylyltransferase -
  BV140_RS02210 (BV140_453) glnB 455782..456120 (+) 339 WP_005649085.1 nitrogen regulatory protein P-II -
  BV140_RS02215 (BV140_454) - 456120..457166 (+) 1047 WP_005686777.1 AI-2E family transporter -
  BV140_RS02220 (BV140_455) priA 457208..459400 (-) 2193 WP_005686778.1 primosomal protein N' Machinery gene
  BV140_RS02225 (BV140_456) trmB 459485..460225 (+) 741 WP_005686779.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  BV140_RS02230 (BV140_457) - 460310..460654 (+) 345 WP_005659951.1 YggL family protein -
  BV140_RS02235 (BV140_458) napF 460860..461390 (+) 531 WP_005686780.1 ferredoxin-type protein NapF -
  BV140_RS02240 (BV140_459) - 461383..461664 (+) 282 WP_005686781.1 chaperone NapD -
  BV140_RS02245 (BV140_460) napA 461701..464184 (+) 2484 WP_005686782.1 nitrate reductase catalytic subunit NapA -

Sequence

Protein


Download         Length: 730 a.a.        Molecular weight: 82422.63 Da        Isoelectric Point: 9.2254

>NTDB_id=183390 BV140_RS02220 WP_005686778.1 457208..459400(-) (priA) [Haemophilus influenzae strain 6P32H1]
MKIVRVVLAVPLPRLFDYLVPDDVSLQIGMRVLVPFGTQKRVAIVADFPTKSDVPEDKLKAILQPLDLAPLFTPIYWDWL
HWAANYYQAGLGDVLFQALPVKLRNGESAVKNDRTFWRITDAGKNALEQGELKRSKKQAEALQYLSETDLEKGNNNFSSA
IWSALKAKGFIEEITIQTKPLSWQQNLGDNPIVNAENRLTLNKQQALAFSQLRFHSGFNVWLLDGVTGSGKTEIYLQYIE
EILKSGKQVLVLVPEIGLTPQTVHRFKARFNVEIDVLHSNLTDTQRLYVWDRARSGQSAIVIGTRSALFTQFSNLGAIIL
DEEHDTSYKQQDSWRYHARDLATVLAQKLNIAVLMGSATPSLESINNVQNGKYQHLVLSKRAGNSTALRHFVIDLKNQHI
QNGLSKPLLERMKAHLEKGNQVLLFLNRRGFAPVLLCHECGWIAQCPHCEKPYTYHQHQNVLRCHHCGAQKTIPRQCGDC
GSTHLVTTGLGTEQLEETLKTLFPHYSVARIDRDSTARKGKLESYLEDIQQGKSQILIGTQMLAKGHHFPNVTLVALVNV
DSALFSLDFRAEERLAQLYIQVAGRAGRADKQGEVVLQTHYPDHPLLTTLLANGYQAFAKETLQLRHSMGLPPFTFQALF
KAQARHSELAQNCLIQIADFFQSKQITGLQMLGPMPAPFSKKAGQYRWQLLLQHPSRMTLQKALREYQQAELEKNSQVRL
ILDVDPQDLS

Nucleotide


Download         Length: 2193 bp        

>NTDB_id=183390 BV140_RS02220 WP_005686778.1 457208..459400(-) (priA) [Haemophilus influenzae strain 6P32H1]
ATGAAAATTGTCCGAGTGGTGCTGGCTGTGCCACTTCCTCGTTTATTTGATTATCTCGTCCCCGATGATGTTTCATTACA
AATCGGTATGCGTGTTCTTGTGCCTTTTGGCACGCAAAAACGTGTCGCCATTGTGGCTGATTTCCCGACAAAATCGGACG
TGCCAGAGGATAAGCTCAAAGCCATTCTTCAACCATTAGATTTAGCACCGCTCTTTACCCCAATTTATTGGGATTGGTTG
CATTGGGCAGCAAATTATTATCAAGCGGGATTAGGCGATGTGTTATTTCAAGCCTTGCCTGTGAAATTACGCAACGGCGA
AAGTGCGGTGAAAAATGACCGCACTTTTTGGCGAATTACGGATGCAGGGAAAAATGCCCTTGAACAAGGCGAATTAAAAC
GCTCGAAAAAGCAAGCTGAAGCCCTCCAATACTTATCTGAAACTGATCTTGAAAAAGGCAATAATAATTTTTCCTCTGCC
ATTTGGTCAGCCTTAAAAGCCAAAGGGTTTATTGAGGAAATTACTATCCAAACAAAGCCCCTAAGCTGGCAACAAAACTT
AGGCGATAACCCAATTGTCAATGCTGAAAATCGTTTAACCTTAAATAAGCAGCAAGCCTTAGCGTTTAGTCAATTGCGTT
TTCATTCTGGTTTCAATGTATGGCTGCTCGATGGGGTGACGGGTTCAGGCAAAACAGAAATTTATCTGCAGTATATTGAA
GAAATTTTAAAATCGGGTAAGCAAGTATTAGTGCTTGTGCCCGAAATCGGGCTAACACCTCAAACGGTGCACCGTTTTAA
AGCACGTTTTAATGTAGAAATTGATGTTCTTCATTCTAATTTAACCGATACACAACGGCTTTATGTATGGGATCGTGCCC
GCTCTGGACAAAGTGCCATTGTGATTGGCACGAGATCGGCATTGTTCACGCAATTTTCTAATCTTGGTGCAATTATTTTG
GATGAAGAACACGATACATCTTACAAACAGCAAGATAGTTGGCGTTACCATGCGCGAGATTTAGCAACCGTACTTGCTCA
AAAACTAAATATTGCGGTATTAATGGGCTCCGCCACGCCAAGTTTAGAAAGTATTAATAATGTACAAAACGGCAAATACC
AACATTTAGTTTTATCAAAAAGAGCGGGAAATTCTACCGCACTTCGTCATTTCGTTATTGATTTGAAAAATCAACACATA
CAAAACGGGCTTTCAAAACCCTTATTGGAACGAATGAAAGCTCACCTCGAAAAAGGCAATCAAGTGTTGCTTTTCTTAAA
TCGTCGAGGCTTTGCACCCGTATTGCTTTGCCATGAATGTGGTTGGATCGCACAATGCCCTCATTGTGAAAAACCTTATA
CCTATCATCAGCATCAAAATGTATTGCGTTGTCATCATTGTGGCGCTCAGAAAACTATTCCTCGTCAATGTGGCGATTGC
GGCTCGACTCATTTAGTCACCACAGGTCTCGGCACGGAACAATTAGAAGAAACCTTGAAAACGCTATTTCCTCATTATTC
TGTAGCTCGAATCGACCGCGATAGCACTGCGCGTAAAGGAAAATTGGAGAGTTATTTAGAAGATATTCAACAAGGCAAAA
GTCAAATTTTGATTGGCACACAAATGCTGGCGAAAGGACATCATTTTCCGAATGTTACCTTGGTTGCCTTAGTGAATGTA
GATAGTGCATTATTTTCGCTAGATTTTCGTGCAGAAGAACGTTTAGCTCAACTTTATATTCAAGTTGCTGGACGAGCTGG
ACGTGCCGATAAACAAGGCGAAGTGGTGCTACAAACACATTATCCAGATCATCCTTTGCTTACCACGTTATTAGCGAATG
GCTACCAAGCTTTTGCAAAAGAAACCTTACAATTACGCCATTCAATGGGATTGCCACCTTTCACTTTTCAGGCGTTATTT
AAAGCACAGGCTCGACATTCTGAGCTTGCACAAAATTGCTTAATCCAGATCGCAGATTTTTTTCAATCAAAGCAAATCAC
GGGATTGCAAATGCTTGGCCCTATGCCTGCACCGTTCAGCAAGAAAGCGGGGCAATATCGCTGGCAGTTATTATTGCAAC
ATCCATCAAGAATGACGTTGCAAAAAGCATTGAGAGAATATCAACAAGCCGAACTTGAAAAAAACAGCCAAGTGCGGTTA
ATTTTGGACGTCGATCCACAAGACTTATCATAA

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(503-589)

DEAD/DEAH box helicase (DEAD)

(221-366)

3DNA-binding domain (3BD) (PriA_3primeBD)

(5-100)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-471)

Primosomal protein N C-terminal domain (PriA_C)

(633-726)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q19V35

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.22

100

0.451

Multiple sequence alignment