Detailed information NTG0183051

Bioinformatically predicted

Overview

• Name: sepM
• Type: Regulator
• Locus tag: LLJM3_RS11975
• Genome accession: NZ_CP015901
• Coordinates: 2309570..2310601 (-)
• Length: 343 a.a.
• NCBI ID: WP_011677213.1
• Uniprot ID: A0A166ZHD8
• Organism: Lactococcus cremoris strain JM3
• Function: processing of CSP (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	2302447..2309362	2309570..2310601	flank	208

Gene organization within MGE regions

Location: 2302447..2310601

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLJM3_RS11950 (LLJM3_2439)	adhE	2302598..2305309 (+)	2712	WP_011677209.1	bifunctional acetaldehyde-CoA/alcohol dehydrogenase	-
LLJM3_RS11955 (LLJM3_2441)	-	2305773..2306270 (-)	498	WP_011677211.1	nitroreductase family protein	-
LLJM3_RS11960 (LLJM3_2442)	-	2306257..2306466 (-)	210	WP_011677212.1	hypothetical protein	-
LLJM3_RS11965 (LLJM3_2444)	-	2306754..2307644 (+)	891	WP_011675620.1	IS982-like element IS982B family transposase	-
LLJM3_RS11970 (LLJM3_2445)	kwcM	2307751..2309070 (-)	1320	WP_014573366.1	KxxxW cyclic peptide radical SAM maturase	-
LLJM3_RS13935	-	2309138..2309212 (-)	75	Protein_2329	KxxxW-cyclized peptide pheromone	-
LLJM3_RS11975 (LLJM3_2446)	sepM	2309570..2310601 (-)	1032	WP_011677213.1	SepM family pheromone-processing serine protease	Regulator

Sequence

Protein

• Length: 343 a.a.
• Molecular weight: 37398.03 Da
• Isoelectric Point: 9.8499

>NTDB_id=183051 LLJM3_RS11975 WP_011677213.1 2309570..2310601(-) (sepM) [Lactococcus cremoris strain JM3]
MKKKNKKISPKLKWGISIGLIIVALLVLVYPTNYYVEMPGTTEPLGKMVKVEGKKDEHKGDFFLTTVQIARANLATMIYS
HFNSFTSIYSEQEMTGGLNDAQFNRVNQFYMETAQNTAVYQAFKLANKPYELKYEGVYVLDIAKNSTFKNKLELSDTITA
VNGEEFKSSADMIAYVSKQKVGDSVTIEYTRIDGSKHKSTGKYIKIANGKTGIGIGLVDHTEVVTDPKVTVNAGSIGGPS
AGIMFTLEIYSQLTGKDLRGGREIAGTGTIEHDGSIGQIGGVDKKVATASKEGAKVFLVPDSGTKKESSNNYLGAKAAAK
KLKTKMKIVPVKTIQDALDYLEK

Nucleotide

• Length: 1032 bp

>NTDB_id=183051 LLJM3_RS11975 WP_011677213.1 2309570..2310601(-) (sepM) [Lactococcus cremoris strain JM3]
ATGAAGAAAAAAAATAAAAAAATCAGTCCCAAATTAAAATGGGGCATCTCTATTGGTCTGATTATTGTCGCTCTCCTTGT
TTTAGTGTATCCAACAAATTACTATGTGGAAATGCCTGGAACAACCGAGCCATTAGGAAAAATGGTCAAAGTCGAAGGAA
AAAAAGATGAGCACAAAGGTGACTTTTTCCTTACTACCGTTCAAATTGCGCGCGCTAATCTTGCCACAATGATTTACAGT
CATTTTAATAGTTTTACAAGCATTTACAGTGAACAAGAAATGACCGGTGGCCTTAATGATGCGCAGTTCAATCGTGTCAA
CCAGTTTTACATGGAAACGGCACAAAATACAGCCGTTTATCAGGCCTTCAAGTTGGCAAATAAACCCTATGAACTGAAAT
ATGAAGGGGTTTATGTCCTTGATATCGCTAAAAATTCGACTTTTAAAAATAAATTGGAACTTTCGGACACGATTACAGCA
GTCAATGGGGAAGAATTTAAATCAAGTGCAGATATGATTGCTTACGTTTCGAAGCAAAAAGTCGGCGATTCAGTTACCAT
TGAATATACACGGATTGATGGAAGTAAACACAAGTCTACTGGAAAATATATCAAAATCGCTAATGGAAAAACTGGAATCG
GAATTGGACTAGTTGACCATACTGAAGTAGTCACAGACCCAAAAGTTACAGTCAATGCAGGTTCAATCGGTGGACCATCT
GCCGGAATAATGTTTACACTAGAGATTTACAGTCAGTTGACAGGTAAAGATTTACGTGGTGGTCGCGAAATAGCAGGAAC
TGGAACGATTGAACATGATGGAAGCATTGGCCAAATCGGTGGGGTTGATAAAAAAGTAGCTACTGCTAGCAAAGAAGGCG
CAAAAGTTTTCTTAGTTCCTGACTCAGGCACCAAAAAAGAAAGCAGCAATAACTATCTTGGAGCCAAAGCAGCCGCTAAA
AAATTAAAAACGAAGATGAAAATTGTTCCCGTCAAGACTATTCAGGACGCTTTAGATTATTTAGAAAAATAA

Domains

Predicted by InterproScan.

• Lon protease (S16) C-terminal proteolytic domain (Lon_C) (229-339)

• PDZ domain (PDZ_2) (135-193)

3D structure

• AlphaFold DB: A0A166ZHD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
sepM	Streptococcus mutans UA159	48.696	100	0.49