Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilK   Type   Machinery gene
Locus tag   AT729_RS08305 Genome accession   NZ_CP015886
Coordinates   1456513..1457106 (-) Length   197 a.a.
NCBI ID   WP_002249142.1    Uniprot ID   -
Organism   Neisseria meningitidis strain 38277     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1440658..1459392 1456513..1457106 within 0
IScluster/Tn 1454282..1455737 1456513..1457106 flank 776


Gene organization within MGE regions


Location: 1440658..1459392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT729_RS08170 (AT729_01369) - 1440658..1440858 (+) 201 WP_002219431.1 hypothetical protein -
  AT729_RS08175 (AT729_01370) - 1440926..1441285 (+) 360 WP_002220694.1 hypothetical protein -
  AT729_RS08180 (AT729_01371) - 1441310..1442164 (+) 855 WP_002220693.1 YfdQ family protein -
  AT729_RS08185 (AT729_01372) - 1442236..1442451 (+) 216 WP_002220692.1 hypothetical protein -
  AT729_RS08190 (AT729_01373) - 1442487..1442747 (+) 261 WP_002220690.1 NGO1622 family putative holin -
  AT729_RS08195 (AT729_01374) - 1442744..1443037 (+) 294 WP_002220689.1 hypothetical protein -
  AT729_RS08200 (AT729_01375) - 1443040..1443231 (+) 192 WP_002219435.1 type ISP restriction/modification enzyme -
  AT729_RS08205 (AT729_01376) - 1443231..1443371 (+) 141 WP_002220688.1 hypothetical protein -
  AT729_RS08210 (AT729_01377) - 1443368..1443577 (+) 210 WP_002220687.1 hypothetical protein -
  AT729_RS08215 (AT729_01378) - 1443635..1444219 (-) 585 Protein_1383 KilA-N domain-containing protein -
  AT729_RS08225 (AT729_01379) - 1444360..1445367 (-) 1008 WP_079889368.1 IS5 family transposase -
  AT729_RS08230 (AT729_01380) - 1445429..1445776 (-) 348 Protein_1385 KilA-N domain-containing protein -
  AT729_RS08235 (AT729_01381) - 1446644..1447123 (-) 480 WP_002241413.1 DUF4760 domain-containing protein -
  AT729_RS08245 (AT729_01382) - 1447559..1447939 (-) 381 WP_002220683.1 type II toxin-antitoxin system PemK/MazF family toxin -
  AT729_RS08250 (AT729_01384) - 1448094..1449292 (-) 1199 Protein_1388 tyrosine-type recombinase/integrase -
  AT729_RS08265 (AT729_01387) yaaA 1449849..1450628 (-) 780 WP_002235690.1 peroxide stress protein YaaA -
  AT729_RS14615 - 1450677..1450802 (-) 126 Protein_1390 IS5/IS1182 family transposase -
  AT729_RS08270 (AT729_01388) dapC 1450891..1452078 (-) 1188 WP_079889515.1 succinyldiaminopimelate transaminase -
  AT729_RS08275 (AT729_01389) dut 1452154..1452606 (-) 453 WP_002222651.1 dUTP diphosphatase -
  AT729_RS08280 (AT729_01390) - 1452751..1453179 (+) 429 Protein_1393 AzlC family ABC transporter permease -
  AT729_RS08290 - 1453857..1454608 (+) 752 WP_195182867.1 IS5-like element IS1301 family transposase -
  AT729_RS08295 - 1454986..1455737 (+) 752 WP_096001759.1 IS5-like element IS1301 family transposase -
  AT729_RS08300 (AT729_01395) pilL 1456011..1456508 (-) 498 WP_002249143.1 PilX family type IV pilin Machinery gene
  AT729_RS08305 (AT729_01396) pilK 1456513..1457106 (-) 594 WP_002249142.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  AT729_RS08310 (AT729_01397) pilJ 1457085..1458074 (-) 990 WP_079889516.1 PilW family protein Machinery gene
  AT729_RS08315 (AT729_01398) pilV 1458071..1458694 (-) 624 WP_079889657.1 type IV pilus modification protein PilV Machinery gene
  AT729_RS08320 (AT729_01399) pilH 1458727..1459392 (-) 666 WP_079889517.1 Tfp pilus assembly protein FimT/FimU Machinery gene

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 21699.58 Da        Isoelectric Point: 5.4116

>NTDB_id=182790 AT729_RS08305 WP_002249142.1 1456513..1457106(-) (pilK) [Neisseria meningitidis strain 38277]
MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGELQVLDLEYDT
DSKVTFSENCEKGLCTAVNVRTNNDNEEAFDNIVVQGKPTVEAVKRSCTANSTDLCIDKKGMEYKKGKGSVSKPPRYIIE
YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE

Nucleotide


Download         Length: 594 bp        

>NTDB_id=182790 AT729_RS08305 WP_002249142.1 1456513..1457106(-) (pilK) [Neisseria meningitidis strain 38277]
ATGCGCAAACAGAACACTTTGACGGGAATCCCGACTTCTGACGGACAGAGGGGGTTTGCACTGTTTATCGTGCTGATGGT
GATGATCGTCGTGGCTTTTTTGGTTGTAACTGCCGCGCAGTCTTACAATACCGAGCAGCGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTGGCCGAGGCGGCTTTGCGGGAAGGCGAACTTCAGGTTTTGGATTTGGAATATGATACG
GACAGTAAGGTTACATTTAGCGAAAACTGTGAAAAAGGTCTGTGTACCGCAGTGAATGTGCGGACAAATAATGATAATGA
AGAGGCTTTTGACAATATCGTGGTGCAAGGCAAGCCCACCGTTGAGGCGGTGAAGCGTTCTTGCACTGCAAATTCTACCG
ACCTGTGCATTGACAAGAAAGGGATGGAATATAAGAAAGGCAAGGGAAGTGTCAGCAAACCACCACGCTATATTATCGAA
TATTTGGGCGTGAAGAACGGAGAAAATGTTTATCGGGTTACTGCCAAGGCTTGGGGTAAGAATGCCAATACCGTGGTCGT
CCTTCAATCTTATGTAAGCAATAATGATGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilX C-term (PilX)

(92-192)

PilX N-terminal (PilX_N)

(17-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilK Neisseria gonorrhoeae MS11

86.634

100

0.888

Multiple sequence alignment