Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   DA68_RS21875 Genome accession   NZ_CP015727
Coordinates   4369348..4370697 (-) Length   449 a.a.
NCBI ID   WP_025966390.1    Uniprot ID   -
Organism   Bacillus cereus strain A1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4364348..4375697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA68_RS21840 (DA68_21840) - 4364406..4365101 (-) 696 WP_025966386.1 hypothetical protein -
  DA68_RS21845 (DA68_21845) - 4365088..4365876 (-) 789 WP_025966387.1 hypothetical protein -
  DA68_RS21850 (DA68_21850) - 4365904..4366473 (-) 570 WP_001211058.1 hypothetical protein -
  DA68_RS21855 (DA68_21855) - 4366578..4367024 (-) 447 WP_025966388.1 hypothetical protein -
  DA68_RS21860 (DA68_21860) raiA 4367455..4367997 (-) 543 WP_000671185.1 ribosome-associated translation inhibitor RaiA -
  DA68_RS21865 (DA68_21865) cspC 4368320..4368517 (-) 198 WP_001990088.1 cold shock protein CspC -
  DA68_RS21870 (DA68_21870) - 4368644..4369348 (-) 705 WP_025966389.1 ComF family protein -
  DA68_RS21875 (DA68_21875) comFA 4369348..4370697 (-) 1350 WP_025966390.1 ATP-dependent helicase ComFA Machinery gene
  DA68_RS21880 (DA68_21880) - 4370825..4372252 (-) 1428 WP_000499468.1 C40 family peptidase -
  DA68_RS21885 (DA68_21885) - 4372401..4372715 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  DA68_RS21890 (DA68_21890) - 4372887..4373729 (-) 843 WP_000684725.1 DegV family protein -
  DA68_RS21895 (DA68_21895) - 4373966..4374601 (+) 636 WP_000926665.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50950.79 Da        Isoelectric Point: 9.9712

>NTDB_id=181845 DA68_RS21875 WP_025966390.1 4369348..4370697(-) (comFA) [Bacillus cereus strain A1]
MLAGKQLLLEELSSDLQRELNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCAYCRKCITMGRVS
ECAVLVRGIAERKREKNSNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKSPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=181845 DA68_RS21875 WP_025966390.1 4369348..4370697(-) (comFA) [Bacillus cereus strain A1]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGGAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCGCATATTGCCGGAAGTGTATAACGATGGGGAGAGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTCAAATTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAAAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACTACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCGTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCATCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATCCCCTATTTTTTTA
TTCGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCAGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAAGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(312-408)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.357

98.441

0.506
  comFA Latilactobacillus sakei subsp. sakei 23K

41.439

89.755

0.372

Multiple sequence alignment