Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   DA68_RS02715 Genome accession   NZ_CP015727
Coordinates   528974..530800 (+) Length   608 a.a.
NCBI ID   WP_000003348.1    Uniprot ID   -
Organism   Bacillus cereus strain A1     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 523974..535800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA68_RS02695 (DA68_02695) - 524277..524951 (-) 675 WP_000362615.1 TerC family protein -
  DA68_RS02700 (DA68_02700) mecA 525297..525980 (+) 684 WP_000350716.1 adaptor protein MecA Regulator
  DA68_RS02705 (DA68_02705) - 526053..527597 (+) 1545 WP_000799192.1 cardiolipin synthase -
  DA68_RS02710 (DA68_02710) - 527678..528922 (+) 1245 WP_000628329.1 competence protein CoiA family protein -
  DA68_RS02715 (DA68_02715) pepF 528974..530800 (+) 1827 WP_000003348.1 oligoendopeptidase F Regulator
  DA68_RS30640 - 530932..531105 (-) 174 WP_000750355.1 hypothetical protein -
  DA68_RS02720 (DA68_02720) - 531335..532228 (-) 894 WP_000360656.1 ClpXP adapter SpxH family protein -
  DA68_RS02725 (DA68_02725) - 532228..532626 (-) 399 WP_000043386.1 hypothetical protein -
  DA68_RS02730 (DA68_02730) - 532795..533373 (-) 579 WP_000191120.1 CYTH domain-containing protein -
  DA68_RS02735 (DA68_02735) - 533518..533889 (+) 372 WP_001180004.1 hypothetical protein -
  DA68_RS02740 (DA68_02740) - 533920..534558 (+) 639 WP_001081489.1 GTP pyrophosphokinase family protein -
  DA68_RS02745 (DA68_02745) - 534577..535374 (+) 798 WP_000673182.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70199.57 Da        Isoelectric Point: 4.6443

>NTDB_id=181821 DA68_RS02715 WP_000003348.1 528974..530800(+) (pepF) [Bacillus cereus strain A1]
MSEQNKAKALPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSSSSNTFGMLNNADLKFPSIKGEDGEEIEITHGRYIQFLESDDPRVRKDAFKAVYETYGKFKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDLLLKSLNVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=181821 DA68_RS02715 WP_000003348.1 528974..530800(+) (pepF) [Bacillus cereus strain A1]
ATGTCTGAACAAAACAAAGCGAAAGCATTACCAGATCGCAACGAGATTGAAGAAGCAAGTACGTGGCGCTTAGAAGATAT
CTTCCAAACGGACGCAGAATGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTGCCGAAGTTAACTGAATTTAAAGGGA
AACTTGGTGACTCTGCGGACAATTTACTTGAGGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGTTATAT
ACATATGCTCATATGCGTTACGATCAAGATACAACAAACTCTGTATATCAAGCATTAAATGATCGCGCAACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCGTATATTGTGCCTGAAATTTTATCGATTTCAGAAGATACACTGCAAACATTCTTGA
AGGAAAATAGAGATTTAAGTGTATATGAACATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTCTTATCAGAAGCT
GAGGAAGCTTTATTAGCGGAAGCATCTGAAGTAATGAGTTCATCAAGCAATACATTCGGTATGTTGAATAACGCGGATTT
AAAATTCCCATCTATTAAAGGGGAAGACGGAGAAGAAATAGAAATTACACATGGCCGTTACATTCAGTTTTTAGAAAGTG
ATGATCCTCGTGTTCGCAAAGATGCATTTAAAGCTGTATATGAAACGTACGGAAAATTTAAAAACACATTCGCAAGTACG
TTAAGCGGAGCAGTAAAACGTAATAATTTCAATGCGCGTGTTCGTAAATATGATTCTGCTCGCCAAGCTGCACTGAGCAA
TAATAATATTCCGGAAGCAGTATACGATCAACTCGTTGAAACAGTAAATGACAACTTACATTTATTACACCGTTATATCG
ATATTCGTAAGCGTGCACTAGGACTTGATGAGCTTCATATGTATGATTTATATACACCACTTGTACCAGAAGTGAAAATG
AATGTGAAATACGAAGAAGCGCAAGACCTTTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGATATTTTGAA
AGAGGCATATGAAAATCGCTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGTGCATATTCATCTGGTGCATATG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAGTTTGGTCATTCG
GTGCATAGTTACTATACAAGAAAAACACAACCGCACGTATATGGTGATTATTCCATCTTCGTAGCAGAAGTGGCATCTAC
TTGTAATGAAGCGCTTCTAAATGATTATTTATTAAAAACGACAGAAGATAAGAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGGTTCCGTGGTACTGTATTCCGTCAAACGATGTTTGCAGAGTTTGAACATATTATTCATAAGAAAGTACAA
GAAGGACATGCTGTTACGCCAGACATGCTAACGGAAATTTACTACGATTTAAATAAGAAATATTTCGGTGACGCTTTAGT
AATCGACGAAGAGATTGGTTTAGAATGGTCTCGTATTCCACACTTCTACTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCAGCGACAGCTCTATCTAAACAGATTTTAGAAGAAGGACAACCAGCAGTAGAACGTTATATTAACGAGTTC
TTAAAAGCAGGAAGCTCTGATTATCCAATTGAAGTGCTGAAAAAAGCAGGAGTAGATATGGCATCTCCTGAACCAGTAAA
AGAGGCGCTACAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.084

97.533

0.488

Multiple sequence alignment