Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A7317_RS25520 Genome accession   NZ_CP015637
Coordinates   5734853..5736220 (+) Length   455 a.a.
NCBI ID   WP_069077072.1    Uniprot ID   A0AB36Y2N8
Organism   Pseudomonas fluorescens strain L321     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5729853..5741220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7317_RS25495 (A7317_25470) - 5730041..5730751 (-) 711 WP_069077071.1 helix-turn-helix transcriptional regulator -
  A7317_RS25500 (A7317_25475) - 5731157..5731933 (+) 777 WP_024077505.1 ferredoxin--NADP reductase -
  A7317_RS25505 (A7317_25480) mscL 5731978..5732394 (-) 417 WP_017737414.1 large-conductance mechanosensitive channel protein MscL -
  A7317_RS25510 (A7317_25485) katB 5732682..5734223 (+) 1542 WP_024077504.1 catalase KatB -
  A7317_RS25515 (A7317_25490) - 5734278..5734817 (+) 540 WP_024077503.1 ankyrin repeat domain-containing protein -
  A7317_RS25520 (A7317_25495) radA 5734853..5736220 (+) 1368 WP_069077072.1 DNA repair protein RadA Machinery gene
  A7317_RS25525 (A7317_25500) - 5736237..5736962 (+) 726 WP_069077458.1 hypothetical protein -
  A7317_RS25530 (A7317_25505) - 5736967..5737335 (-) 369 WP_024077500.1 PilZ domain-containing protein -
  A7317_RS25535 (A7317_25510) - 5737509..5739575 (+) 2067 WP_024077499.1 carbon starvation CstA family protein -
  A7317_RS25540 (A7317_25515) - 5739591..5739788 (+) 198 WP_003176208.1 YbdD/YjiX family protein -
  A7317_RS25545 (A7317_25520) yjiA 5739924..5740883 (+) 960 WP_024077498.1 GTPase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48759.23 Da        Isoelectric Point: 6.8987

>NTDB_id=181578 A7317_RS25520 WP_069077072.1 5734853..5736220(+) (radA) [Pseudomonas fluorescens strain L321]
MAKAKRMYGCTECGATFPKWAGQCTECGAWNTLTETMIESGGASAPTGRAGWTGQQAQIKTLAEVSVEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCSIASRMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIESIIA
TARIEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFAMTDRGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQVIGVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=181578 A7317_RS25520 WP_069077072.1 5734853..5736220(+) (radA) [Pseudomonas fluorescens strain L321]
ATGGCAAAGGCCAAGCGCATGTACGGCTGCACGGAGTGCGGCGCGACCTTTCCCAAGTGGGCCGGCCAATGCACCGAGTG
CGGTGCGTGGAATACCCTCACGGAAACCATGATCGAAAGTGGCGGCGCCTCGGCCCCTACCGGCCGCGCCGGCTGGACCG
GGCAACAGGCGCAGATCAAGACCCTGGCTGAAGTCAGTGTCGAAGAGATTCCGCGTTTCTCCACCGCGTCCGGCGAGCTG
GACCGTGTGCTCGGCGGCGGCCTGGTGGATGGTTCGGTGGTGCTGATCGGCGGCGACCCGGGCATCGGCAAGTCGACGAT
CCTGCTGCAAACCCTGTGCAGCATCGCCAGCCGCATGCCGGCGCTGTACGTCACGGGTGAAGAATCCCAGCAGCAAGTGG
CCATGCGCGCCCGTCGTTTGGGCCTGCCCCAGGATCAACTGCGGGTCATGACGGAAACCTGCATCGAAAGCATCATCGCC
ACGGCCCGCATCGAAAAACCCAAGGTCATGGTGATCGACTCGATCCAGACGATTTTCACCGAACAACTGCAATCGGCGCC
GGGTGGCGTGTCCCAGGTGCGCGAAAGTGCGGCGCTGCTGGTGCGTTATGCCAAGCAGAGTGGCACGGCGATCTTCCTGG
TTGGCCACGTGACCAAGGAAGGTGCATTGGCCGGGCCACGCGTGCTGGAGCATATGGTCGACACCGTGCTGTATTTCGAA
GGTGAATCCGATGGCCGCCTGCGTTTGCTGCGCGCGGTGAAGAACCGCTTTGGCGCGGTGAACGAGCTGGGTGTGTTCGC
CATGACCGACCGGGGGCTGAAAGAAGTCTCCAATCCATCGGCGATTTTTCTCACCCGTGCCCAGGAAGAAGTCCCCGGCA
GTGTGGTGATGGCGACGTGGGAAGGCACTCGGCCGATGCTGGTGGAAGTCCAGGCGCTGGTGGATGACAGCCATTTGGCC
AACCCGCGTCGAGTCACCCTGGGCCTGGATCAGAACCGCCTGGCGATGTTGCTGGCGGTATTGCACCGCCATGGTGGCAT
TCCGACCCATGACCAGGACGTGTTCCTCAACGTGGTGGGCGGGGTCAAGGTGCTGGAAACCGCATCCGACCTGGCGTTGA
TGGCGGCGGTGATGTCCAGCCTGCGCAACCGGCCGCTGCCCCATGACTTGCTGGTGTTTGGCGAAGTCGGCCTGTCGGGC
GAAGTGCGCCCGGTGCCCAGTGGCCAGGAGCGCCTCAAGGAAGCCGCCAAGCACGGCTTCAAACGCGCGATTGTGCCGAA
GGGCAATGCGCCAAAGGAAGCGCCGCCAGGGTTGCAGGTGATCGGGGTAACGCGCCTGGAACAGGCATTGGATGCACTTT
TCGAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(80-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.672

100

0.499
  radA Streptococcus pneumoniae Rx1

46.957

100

0.475
  radA Streptococcus pneumoniae R6

46.957

100

0.475
  radA Streptococcus pneumoniae TIGR4

46.957

100

0.475
  radA Streptococcus pneumoniae D39

46.957

100

0.475
  radA Streptococcus mitis SK321

47.149

100

0.473
  radA Streptococcus mitis NCTC 12261

46.93

100

0.47

Multiple sequence alignment