Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AsACE_RS02615 Genome accession   NZ_CP015615
Coordinates   557675..558475 (+) Length   266 a.a.
NCBI ID   WP_076753067.1    Uniprot ID   A0A1P8PHG5
Organism   Acinetobacter schindleri strain ACE     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 552675..563475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AsACE_RS02590 (AsACE_CH00512) - 554439..554849 (+) 411 WP_004890015.1 hypothetical protein -
  AsACE_RS02595 (AsACE_CH00513) - 554919..555470 (+) 552 WP_048881427.1 phosphatase PAP2 family protein -
  AsACE_RS02600 (AsACE_CH00514) iscX 555560..555760 (+) 201 WP_004813114.1 Fe-S cluster assembly protein IscX -
  AsACE_RS02605 (AsACE_CH00515) ndk 555871..556302 (+) 432 WP_004813112.1 nucleoside-diphosphate kinase -
  AsACE_RS02610 (AsACE_CH00516) rlmN 556425..557660 (+) 1236 WP_076753066.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AsACE_RS02615 (AsACE_CH00517) pilF 557675..558475 (+) 801 WP_076753067.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AsACE_RS02620 (AsACE_CH00518) - 558466..559251 (+) 786 WP_076753068.1 helix-turn-helix domain-containing protein -
  AsACE_RS02625 (AsACE_CH00519) ispG 559275..560396 (+) 1122 WP_076753069.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AsACE_RS02630 (AsACE_CH00520) hisS 560400..561695 (+) 1296 WP_076753070.1 histidine--tRNA ligase -
  AsACE_RS02635 (AsACE_CH00521) - 561726..562424 (+) 699 WP_004889998.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30147.35 Da        Isoelectric Point: 9.4227

>NTDB_id=181513 AsACE_RS02615 WP_076753067.1 557675..558475(+) (pilF) [Acinetobacter schindleri strain ACE]
MRNLTSKIAFISTVCVSIALSGCQTAEVGKKDPKKATQVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVL
LQQEGSRLNLDRAETYFKRAISAEPKNAQARNNYGTYLYQLERYNDAIEQFAIAGATLGYDQRYLALENMGRIYLKQGDL
VNAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAASNMYQQFVRSVGQKNQGARALWIGIRTARAEGDQMGMQVLVNQ
LRALFPESPEYQRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=181513 AsACE_RS02615 WP_076753067.1 557675..558475(+) (pilF) [Acinetobacter schindleri strain ACE]
TTGAGAAATCTGACATCTAAAATTGCTTTTATTTCAACAGTATGTGTTTCTATTGCTCTATCTGGCTGTCAAACGGCAGA
AGTGGGTAAAAAGGATCCTAAAAAAGCGACCCAGGTGCGTACTCAATTGGCGGTTGAATATCTGAAGACTGGTGATCTGG
ATTCAGCGAAAAGAGCGCTAGATGAGGCGCTTAAAATTAATTCACGTGATGCTCAAGCCAATATGATGATGGGTGTTTTG
TTACAGCAGGAAGGTAGTAGGCTGAACTTGGATCGTGCCGAAACTTATTTTAAGCGCGCGATTTCAGCCGAACCTAAAAA
TGCACAGGCACGTAACAATTATGGCACCTATTTGTATCAGCTTGAACGTTATAATGATGCAATTGAACAGTTTGCAATTG
CAGGCGCGACACTGGGCTATGATCAGCGTTATCTTGCGCTTGAAAATATGGGGCGGATCTACCTGAAGCAGGGCGATCTG
GTGAATGCTGAAAAATCATTCAAACAGGCCCTGCAAGCCAATCGCGATTCTTACATTTCAATGTTAGAGTTGGCAGAGAT
TTTTTATCTGAATCAGCAGTTCCCGGCGGCGTCGAATATGTATCAACAATTTGTACGTTCAGTGGGACAGAAAAACCAGG
GTGCTCGTGCGCTCTGGATCGGCATTCGTACTGCGCGTGCCGAGGGTGATCAAATGGGCATGCAAGTATTAGTCAATCAA
TTGCGCGCATTATTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_2)

(146-176)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1P8PHG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.692

97.744

0.759
  pilF Acinetobacter baylyi ADP1

69.141

96.241

0.665

Multiple sequence alignment