Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   BSA41_RS03755 Genome accession   NZ_CP015610
Coordinates   699252..702956 (+) Length   1234 a.a.
NCBI ID   WP_075611208.1    Uniprot ID   -
Organism   Bacillus safensis strain U41     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 694252..707956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSA41_RS03740 (BSA41_03625) - 694557..694967 (+) 411 WP_039177642.1 DoxX family protein -
  BSA41_RS03745 (BSA41_03630) - 695166..695612 (-) 447 WP_073979873.1 ferritin-like domain-containing protein -
  BSA41_RS03750 (BSA41_03635) addB 695765..699274 (+) 3510 WP_075611207.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BSA41_RS03755 (BSA41_03640) addA 699252..702956 (+) 3705 WP_075611208.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BSA41_RS03760 (BSA41_03645) sbcD 702983..704157 (+) 1175 Protein_714 exonuclease subunit SbcD -
  BSA41_RS03765 (BSA41_03650) - 704154..707549 (+) 3396 WP_075611209.1 AAA family ATPase -
  BSA41_RS03770 (BSA41_03655) - 707569..707868 (+) 300 WP_039177660.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142112.16 Da        Isoelectric Point: 4.9853

>NTDB_id=181366 BSA41_RS03755 WP_075611208.1 699252..702956(+) (addA) [Bacillus safensis strain U41]
MQIPKPNNSNWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELISHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFEKAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHAADGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFTPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRSKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPIVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLLPDFERYQSKTYLDFIGPALIRHQAMSSILEETGDVVLSHPSAFTISFTQASDL
LKEDMSLEKKQQDEVVQALMEGLPVEGYGNADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRKAR
ATLYDRPAFMKKKALTAAEQGTAMHTVMQHIALPSDEPYDDSRIQQLLDSLQQRDLLTNEQVQSIDQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGPEAAVPILKKRYE
TQIALYAIAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=181366 BSA41_RS03755 WP_075611208.1 699252..702956(+) (addA) [Bacillus safensis strain U41]
ATGCAAATACCAAAACCGAATAACAGTAATTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCGGCGGCAGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCAGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCGAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCGCTCATGAATCGTGCCAATATTTC
TACCTTGCACTCTTTTTGTTTGCAGGTTCTGCGCACCTTTTATTATGAAATTGACCTTGATCCAGGCTTCCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCG
TTCTTTGAACTGGTTGATCGCTATACTTCTGACCGTCATGATTTAGACTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCACCCTTCACCTGAGCAATGGATGAGGGCATTTCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTATCCATATATCAAAGAGGACCTTTCTCTCGTTTTTCGAAGCTGTCTCGAACTACTCGAACGAGCT
CTCGCCCTATCAAAAGAACCGGGCGGCCCGGCACCGAGAGCAGAAAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCAGTCATCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAACTTAAA
GATGAATACTTTATGCGCAGTCCAGCCCAGCATTTGAAGAGCTTAGCTGAAATGAAGCCGGTGGTCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAAAGCGAAGCAAGAAAAATCAATCGTGGACTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGATGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAAGATACAAACCTTGTACAAGAAACGATTTTAAAACTTGTATCTAAAGGGGA
ACATGCCGCAGACGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGCTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACAATTTACACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTTCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGTAAAGATACAACGACAGAAATGCTTCTTGTCCATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGAGAAGAACTAGAGACCGTACAGCTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGATAT
TGTGATTTTGCTTCGTTCTATGCCATGGGCCCCGCAAATGATGGAGGAATTGAAGAAACAGGGAATCCCTGTGTATGCGA
ATCTTTCTTCCGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTCTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTATTAAGATCACCACTTGTCCATTTAGACGAAAATGAAATGGCACTGATTCGCACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGGTTTTTCAGGTTGCTGCGTAAATGGCGTGATTTCTCGATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTTGGCGGGATGCCTGGAGGCAAGCAGAGACAGGCGAATCTCCGCGCTCTTTATGA
TCGATCAAAGCAGTATGAAAAAGCTGCCTTTAGAGGACTGTTCCGTTTTCTTCGTTTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAAGATGTTGTTCGCATGATGACGATTCACAGCAGTAAAGGG
TTAGAATTTCCAATCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTATTTACTTGA
TAAAGAGCTAGGCTTTGGAAGCAAGTATATCCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTAGCGATGA
AAAAAAAGATGAGGAAAGAGCTATTATCTGAAGAGCTGAGAGTTCTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCTGTGAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAAGATTGGCTGCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTCCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAACAGGTGATGTTGTTCTTTCGCATCCATCAGCATTCACAATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGAAGCAGCAAGACGAGGTGGTACAAGCATTGATGGAAGGCCTGCCTGTTGAAGG
ATATGGTAATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCATATCTAGCAGCCTCACAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAGAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTCGAAAAGCCCGT
GCCACGTTGTATGACCGGCCGGCTTTTATGAAGAAAAAAGCACTCACTGCCGCAGAGCAAGGCACCGCAATGCACACAGT
CATGCAACATATTGCGCTTCCTTCTGACGAGCCCTATGATGATTCACGTATACAGCAGCTGCTTGATTCTTTACAGCAGC
GAGACTTGCTAACAAATGAGCAAGTCCAGTCAATTGATCAAGAAGGAATTGCAGCTTTCTTCTCTACTTCTATTGGTCAA
AAGCTGCTAAAAGCGGACTGGGTAAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGGGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGACGGAAAACTATATTTACTAG
ATTATAAAACCGATAGAGTGCAAGGCCGATATACAGGCGGCCCTGAAGCTGCTGTGCCTATATTGAAAAAGCGCTATGAA
ACTCAAATAGCTTTATATGCAATAGCAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGGAATTTAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-462)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

65.94

100

0.662

Multiple sequence alignment