Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BSA145_RS20650 Genome accession   NZ_CP015607
Coordinates   3946629..3947399 (-) Length   256 a.a.
NCBI ID   WP_075623525.1    Uniprot ID   A0A1L6ZN94
Organism   Bacillus safensis strain U14-5     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 3941629..3952399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSA145_RS20630 (BSA145_20190) - 3941721..3942542 (-) 822 WP_075623522.1 pyruvate, water dikinase regulatory protein -
  BSA145_RS20635 (BSA145_20195) - 3942568..3943206 (-) 639 WP_039180035.1 helix-turn-helix transcriptional regulator -
  BSA145_RS20640 (BSA145_20200) glyS 3943374..3945419 (-) 2046 WP_075623523.1 glycine--tRNA ligase subunit beta -
  BSA145_RS20645 (BSA145_20205) glyQ 3945412..3946299 (-) 888 WP_075623524.1 glycine--tRNA ligase subunit alpha -
  BSA145_RS20650 (BSA145_20210) recO 3946629..3947399 (-) 771 WP_075623525.1 DNA repair protein RecO Machinery gene
  BSA145_RS20655 - 3947435..3947578 (-) 144 WP_003217288.1 YqzL family protein -
  BSA145_RS20660 (BSA145_20215) era 3947727..3948632 (-) 906 WP_007501305.1 GTPase Era -
  BSA145_RS20665 (BSA145_20220) - 3948625..3949023 (-) 399 WP_075623526.1 cytidine deaminase -
  BSA145_RS20670 (BSA145_20225) - 3949137..3949523 (-) 387 WP_075623736.1 diacylglycerol kinase family protein -
  BSA145_RS20675 (BSA145_20230) ybeY 3949501..3949980 (-) 480 WP_041106915.1 rRNA maturation RNase YbeY -
  BSA145_RS20680 (BSA145_20235) - 3949981..3952092 (-) 2112 WP_418202176.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29217.92 Da        Isoelectric Point: 8.7151

>NTDB_id=181356 BSA145_RS20650 WP_075623525.1 3946629..3947399(-) (recO) [Bacillus safensis strain U14-5]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRA
IREDLFLTAYAAYMTELMDKGTEEKKPNPYLFELLLQSLRHLNEGTDANIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=181356 BSA145_RS20650 WP_075623525.1 3946629..3947399(-) (recO) [Bacillus safensis strain U14-5]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGAAAAATTGGCGTGATGGCAAGAGGCGCTAAAAAATCAAACAGCCGCCTGTCAGCTATAAGCCAGCCGTTTT
TATACGGCACCTTTCTCATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGGGCC
ATTCGGGAAGACTTATTTCTGACCGCATATGCCGCATATATGACGGAATTGATGGATAAAGGGACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTTCTGCTTCAATCTCTGCGTCATCTCAACGAAGGTACAGATGCTAATATTATTTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGGATGAAGCCTGAACTTGATCAATGTGTCCACTGTGGTCAAAAAGAAGGG
CAGTTCCATTTCTCAATAAGAGATAACGGCTTCATTTGCCAAAATTGTTTTTCGAAAGATCCATATAAACTGCCCTTATC
CCCCGCAGCTGCAAGGCTGCTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAGGTTGATGTGAAGCCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTTGATCACTACTATGACGAATATTCCGGCCTCTATTTAAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L6ZN94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75

100

0.75

Multiple sequence alignment