Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BSA145_RS00540 Genome accession   NZ_CP015607
Coordinates   74035..75414 (-) Length   459 a.a.
NCBI ID   WP_025092231.1    Uniprot ID   A0AAP7QRW5
Organism   Bacillus safensis strain U14-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 69035..80414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSA145_RS00520 (BSA145_00505) ispF 70516..70992 (-) 477 WP_003216928.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  BSA145_RS00525 (BSA145_00510) ispD 70996..71685 (-) 690 WP_041115162.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BSA145_RS00530 (BSA145_00515) - 71699..72799 (-) 1101 WP_041084746.1 PIN/TRAM domain-containing protein -
  BSA145_RS00535 (BSA145_00520) disA 72953..74032 (-) 1080 WP_024425581.1 DNA integrity scanning diadenylate cyclase DisA -
  BSA145_RS00540 (BSA145_00525) radA 74035..75414 (-) 1380 WP_025092231.1 DNA repair protein RadA Machinery gene
  BSA145_RS00545 (BSA145_00530) clpC 75508..77943 (-) 2436 WP_007496263.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BSA145_RS00550 (BSA145_00535) - 77940..79031 (-) 1092 WP_024425579.1 protein arginine kinase -
  BSA145_RS00555 (BSA145_00540) - 79036..79593 (-) 558 WP_024425862.1 UvrB/UvrC motif-containing protein -
  BSA145_RS00560 (BSA145_00545) - 79608..80072 (-) 465 WP_003217184.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50009.49 Da        Isoelectric Point: 7.3829

>NTDB_id=181299 BSA145_RS00540 WP_025092231.1 74035..75414(-) (radA) [Bacillus safensis strain U14-5]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIESASLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=181299 BSA145_RS00540 WP_025092231.1 74035..75414(-) (radA) [Bacillus safensis strain U14-5]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCGGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGCAAAGAGCCAGCAAACCGTCGAAGTGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCCGAAGAACCCCGAATTAAAACAAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGTTCTCTTGTTCTCATCGGCGGAGATCCTGGGATTGGGAAGTCCACATT
GTTATTACAAGTATCAGCACAGCTCTCAGACAAAAATCAGAATGTGTTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTTGGCATTGAAAGCGCCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAGGAGATGAAACCTGCTTTTGTTGTGGTGGATTCGATTCAAACCGTTTACCAAAGCGATATCACGTCAGC
TCCCGGCTCTGTATCTCAAGTTAGAGAATGTACAGCGCAGCTGATGAAGATTGCCAAAACAAATGGTATTCCTATTTTTA
TCGTTGGTCACGTAACCAAAGAAGGCTCGATCGCAGGTCCACGCCTTTTAGAGCATATGGTGGACACAGTTCTTTATTTT
GAAGGCGAGCGTCATCATACGTTTCGTATCTTACGGGCGGTCAAAAACCGATTTGGTTCAACGAATGAATTGGGAATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAACCCATCAGAAATCTTTTTAGAAGAGCGGTCGGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCCGTACTTGTCGAGATACAGGCATTAATTTCCCCGACAAGCTTT
GGGAATCCGCGAAGAATGGCCACTGGCCTTGATCATAATCGAGTATCGCTGCTCATGGCAGTTTTAGAAAAACGCGTGGG
GCTGCTGCTGCAAAACCAAGATGCCTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCGGCGATTGACTTAGCTA
TTGCTGTCAGTATTGCATCAAGCTTTAAAGATGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGAGTGCAGGAAGCTGCAAAACTAGGATTTAAGCGAATGTTTATTCC
TCAGGCGAATATAGACGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTTGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.336

99.782

0.871
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608

Multiple sequence alignment