Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A3K93_RS01840 Genome accession   NZ_CP015594
Coordinates   404394..405182 (+) Length   262 a.a.
NCBI ID   WP_081408495.1    Uniprot ID   -
Organism   Acinetobacter sp. NCu2D-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 399394..410182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3K93_RS01820 (A3K93_01820) - 400271..402184 (+) 1914 WP_157883249.1 ATP-binding protein -
  A3K93_RS01825 (A3K93_01825) iscX 402279..402479 (+) 201 WP_067728400.1 Fe-S cluster assembly protein IscX -
  A3K93_RS01830 (A3K93_01830) ndk 402590..403021 (+) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  A3K93_RS01835 (A3K93_01835) rlmN 403144..404367 (+) 1224 WP_067728402.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  A3K93_RS01840 (A3K93_01840) pilF 404394..405182 (+) 789 WP_081408495.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  A3K93_RS01845 (A3K93_01845) - 405173..405958 (+) 786 WP_067728406.1 RodZ domain-containing protein -
  A3K93_RS01850 (A3K93_01850) ispG 405982..407103 (+) 1122 WP_067728408.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  A3K93_RS01855 (A3K93_01855) hisS 407107..408402 (+) 1296 WP_067728410.1 histidine--tRNA ligase -
  A3K93_RS01860 (A3K93_01860) - 408434..409132 (+) 699 WP_067728412.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 262 a.a.        Molecular weight: 29532.57 Da        Isoelectric Point: 9.1041

>NTDB_id=181234 A3K93_RS01840 WP_081408495.1 404394..405182(+) (pilF) [Acinetobacter sp. NCu2D-2]
MSKLALISTLCVSVALVACQTQDIAKKDPEKAIKVRTQMAVEFIKSGDLDSAKRVLDEALSIRSRDSQANMMMGVLLQQE
GSALNLQKADAYFKRAISSDSKNAQARNNYGTYLFQVGRYKEAAEQFEIAGTTLGYEQRGRALENLGQSYLKMNDVVNAE
KSFKQALQANRDSYVSMLELAEISYLKQDFQTASRMYQDFVRGVGQNNQNARALWIGIRTARAEGDQMGMQVLVNQLRAL
FPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 789 bp        

>NTDB_id=181234 A3K93_RS01840 WP_081408495.1 404394..405182(+) (pilF) [Acinetobacter sp. NCu2D-2]
ATGTCTAAGCTTGCTTTGATTTCAACACTTTGTGTATCGGTGGCTTTGGTGGCATGTCAAACTCAGGATATTGCAAAAAA
AGATCCTGAAAAAGCCATTAAAGTCCGCACACAGATGGCCGTGGAATTTATTAAAAGTGGTGACCTCGATTCAGCTAAAC
GCGTTTTAGATGAAGCTTTGTCAATTCGATCTCGTGATTCTCAAGCCAACATGATGATGGGTGTACTGCTTCAACAAGAA
GGCAGTGCTTTGAATCTGCAAAAAGCAGATGCCTATTTCAAACGTGCAATTTCAAGTGATTCAAAAAATGCCCAAGCGCG
AAATAACTATGGAACCTACTTATTCCAAGTTGGGCGCTATAAAGAAGCGGCTGAACAGTTTGAAATTGCAGGAACAACAT
TGGGTTATGAGCAACGTGGACGTGCTTTAGAAAATTTGGGGCAGAGTTACCTCAAAATGAATGATGTGGTAAATGCTGAA
AAATCGTTCAAACAAGCCCTACAAGCCAATCGCGATTCTTATGTTTCAATGTTAGAGTTGGCAGAGATTTCTTATCTGAA
ACAAGATTTCCAGACTGCATCACGTATGTATCAAGATTTTGTACGTGGTGTGGGACAAAATAACCAGAATGCACGAGCGC
TCTGGATCGGCATACGTACTGCGCGTGCCGAGGGTGATCAAATGGGCATGCAAGTGTTGGTCAATCAATTACGCGCATTG
TTCCCTGAAAGCCCAGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(85-128)

Tetratricopeptide repeat (TPR_8)

(141-172)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

71.103

100

0.714
  pilF Acinetobacter baylyi ADP1

64.419

100

0.656

Multiple sequence alignment