Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BZY78_RS20855 Genome accession   NZ_CP019560
Coordinates   4071850..4072290 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain KSC1031     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4066850..4077290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BZY78_RS20835 (BZY78_21135) coaE 4067340..4067960 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  BZY78_RS25725 - 4067985..4068029 (+) 45 WP_120795372.1 protein YacM -
  BZY78_RS20840 (BZY78_21140) guaC 4068185..4069228 (+) 1044 WP_001217338.1 GMP reductase -
  BZY78_RS20845 (BZY78_21145) hofC 4069263..4070465 (-) 1203 WP_000157236.1 protein transport protein HofC -
  BZY78_RS20850 (BZY78_21150) pilB 4070455..4071840 (-) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  BZY78_RS20855 (BZY78_21155) pilA 4071850..4072290 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  BZY78_RS20865 (BZY78_21165) nadC 4072493..4073386 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BZY78_RS20870 (BZY78_21170) ampD 4073474..4074025 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BZY78_RS20875 (BZY78_21175) ampE 4074022..4074876 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  BZY78_RS20880 (BZY78_21180) aroP 4074919..4076292 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=180883 BZY78_RS20855 WP_000360895.1 4071850..4072290(-) (pilA) [Escherichia coli strain KSC1031]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=180883 BZY78_RS20855 WP_000360895.1 4071850..4072290(-) (pilA) [Escherichia coli strain KSC1031]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363