Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   A2I63_RS10175 Genome accession   NZ_CP015536
Coordinates   2084749..2085180 (-) Length   143 a.a.
NCBI ID   WP_015899733.1    Uniprot ID   B9DJE5
Organism   Staphylococcus carnosus strain TMW 2.243     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2047525..2093757 2084749..2085180 within 0


Gene organization within MGE regions


Location: 2047525..2093757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A2I63_RS09920 (A2I63_09780) - 2047525..2048325 (-) 801 WP_015899779.1 glycerophosphodiester phosphodiesterase family protein -
  A2I63_RS09925 - 2048405..2048545 (-) 141 WP_015899778.1 hypothetical protein -
  A2I63_RS09930 (A2I63_09785) - 2048538..2049218 (-) 681 WP_015899777.1 hypothetical protein -
  A2I63_RS09935 (A2I63_09795) - 2049912..2051315 (-) 1404 WP_015899776.1 N-acetylmuramoyl-L-alanine amidase -
  A2I63_RS09940 (A2I63_09800) - 2051315..2051578 (-) 264 WP_015899775.1 phage holin -
  A2I63_RS09945 (A2I63_09805) - 2051637..2053742 (-) 2106 WP_015899774.1 BppU family phage baseplate upper protein -
  A2I63_RS09950 (A2I63_09810) - 2053789..2054187 (-) 399 WP_015899773.1 hypothetical protein -
  A2I63_RS09955 (A2I63_09815) - 2054174..2054596 (-) 423 WP_015899772.1 hypothetical protein -
  A2I63_RS09960 - 2054635..2054808 (-) 174 WP_015899771.1 XkdX family protein -
  A2I63_RS09965 (A2I63_09820) - 2054808..2055164 (-) 357 WP_015899770.1 hypothetical protein -
  A2I63_RS09970 (A2I63_09825) - 2055169..2056593 (-) 1425 WP_015899769.1 BppU family phage baseplate upper protein -
  A2I63_RS09975 (A2I63_09830) - 2056596..2058551 (-) 1956 WP_015899768.1 glycosyl hydrolase family 28-related protein -
  A2I63_RS09980 (A2I63_09835) - 2058556..2058774 (-) 219 WP_015899767.1 hypothetical protein -
  A2I63_RS09985 (A2I63_09840) - 2058778..2060331 (-) 1554 WP_253724525.1 prophage endopeptidase tail family protein -
  A2I63_RS09990 (A2I63_09845) - 2060342..2061175 (-) 834 WP_015899765.1 phage tail domain-containing protein -
  A2I63_RS09995 (A2I63_09850) - 2061172..2063454 (-) 2283 WP_253724526.1 peptidoglycan DD-metalloendopeptidase family protein -
  A2I63_RS10000 (A2I63_09855) - 2063435..2065381 (-) 1947 WP_253724527.1 hypothetical protein -
  A2I63_RS10005 (A2I63_09860) - 2065329..2067434 (-) 2106 WP_015899763.1 phage tail tape measure protein -
  A2I63_RS10010 - 2067448..2067603 (-) 156 WP_015899762.1 hypothetical protein -
  A2I63_RS10015 (A2I63_09865) - 2067654..2067998 (-) 345 WP_015899761.1 hypothetical protein -
  A2I63_RS10020 (A2I63_09870) - 2068046..2068507 (-) 462 WP_015899760.1 Ig-like domain-containing protein -
  A2I63_RS10025 (A2I63_09875) - 2068584..2069225 (-) 642 WP_015899759.1 major tail protein -
  A2I63_RS10030 (A2I63_09880) - 2069267..2069662 (-) 396 WP_015899758.1 hypothetical protein -
  A2I63_RS10035 (A2I63_09885) - 2069659..2070066 (-) 408 WP_015899757.1 hypothetical protein -
  A2I63_RS10040 (A2I63_09890) - 2070063..2070419 (-) 357 WP_231839067.1 hypothetical protein -
  A2I63_RS10045 (A2I63_09895) - 2070403..2070699 (-) 297 WP_015899755.1 hypothetical protein -
  A2I63_RS10050 (A2I63_09900) - 2070772..2071890 (-) 1119 WP_015899754.1 phage major capsid protein -
  A2I63_RS10055 (A2I63_09905) - 2071915..2072631 (-) 717 WP_015899753.1 head maturation protease, ClpP-related -
  A2I63_RS10060 (A2I63_09910) - 2072621..2073793 (-) 1173 WP_015899752.1 phage portal protein -
  A2I63_RS10065 (A2I63_09915) - 2073808..2075448 (-) 1641 WP_015899751.1 terminase large subunit -
  A2I63_RS10070 (A2I63_09920) - 2075445..2075792 (-) 348 WP_015899750.1 hypothetical protein -
  A2I63_RS10075 (A2I63_09925) - 2075969..2076301 (-) 333 WP_041612952.1 HNH endonuclease -
  A2I63_RS10080 (A2I63_09930) - 2076596..2077009 (-) 414 WP_015899749.1 hypothetical protein -
  A2I63_RS10085 (A2I63_09935) - 2077022..2077201 (-) 180 WP_015899748.1 hypothetical protein -
  A2I63_RS10090 - 2077335..2077721 (-) 387 WP_041612951.1 hypothetical protein -
  A2I63_RS10095 (A2I63_09945) - 2077725..2078009 (-) 285 WP_015899747.1 hypothetical protein -
  A2I63_RS10100 (A2I63_09950) - 2078002..2078274 (-) 273 WP_041612950.1 hypothetical protein -
  A2I63_RS10105 - 2078416..2078523 (-) 108 Protein_1965 DUF1381 domain-containing protein -
  A2I63_RS10110 (A2I63_09960) - 2078524..2078730 (-) 207 WP_015899745.1 hypothetical protein -
  A2I63_RS10115 (A2I63_09965) - 2078780..2079289 (-) 510 WP_015899744.1 dUTP pyrophosphatase -
  A2I63_RS10120 (A2I63_09970) - 2079290..2079493 (-) 204 WP_041612949.1 hypothetical protein -
  A2I63_RS10125 (A2I63_09975) - 2079486..2079683 (-) 198 WP_015899743.1 hypothetical protein -
  A2I63_RS10130 (A2I63_09980) - 2079730..2080215 (-) 486 WP_015899742.1 nucleoside 2-deoxyribosyltransferase -
  A2I63_RS10135 (A2I63_09985) - 2080208..2080684 (-) 477 WP_015899741.1 DUF3310 domain-containing protein -
  A2I63_RS10140 (A2I63_09990) - 2080685..2081215 (-) 531 WP_015899740.1 hypothetical protein -
  A2I63_RS10145 - 2081221..2081397 (-) 177 WP_015899739.1 hypothetical protein -
  A2I63_RS10150 (A2I63_09995) - 2081394..2081801 (-) 408 WP_015899738.1 RusA family crossover junction endodeoxyribonuclease -
  A2I63_RS10155 (A2I63_10005) - 2081967..2082752 (-) 786 WP_015899737.1 ATP-binding protein -
  A2I63_RS10160 (A2I63_10010) - 2082753..2083514 (-) 762 WP_015899736.1 conserved phage C-terminal domain-containing protein -
  A2I63_RS10165 (A2I63_10015) - 2083511..2084185 (-) 675 WP_015899735.1 putative HNHc nuclease -
  A2I63_RS10170 (A2I63_10020) - 2084186..2084737 (-) 552 WP_015899734.1 NUMOD4 domain-containing protein -
  A2I63_RS10175 (A2I63_10025) ssbA 2084749..2085180 (-) 432 WP_015899733.1 single-stranded DNA-binding protein Machinery gene
  A2I63_RS10180 (A2I63_10030) - 2085180..2085851 (-) 672 WP_015899732.1 ERF family protein -
  A2I63_RS10185 (A2I63_10035) - 2085808..2086068 (-) 261 WP_015899731.1 DUF2483 family protein -
  A2I63_RS10190 (A2I63_10040) - 2086061..2086306 (-) 246 WP_015899730.1 hypothetical protein -
  A2I63_RS10195 (A2I63_10045) - 2086394..2086615 (-) 222 WP_041612947.1 hypothetical protein -
  A2I63_RS10200 (A2I63_10050) - 2086612..2086848 (-) 237 WP_015899729.1 hypothetical protein -
  A2I63_RS10205 (A2I63_10055) - 2086912..2087184 (+) 273 WP_015899728.1 hypothetical protein -
  A2I63_RS10210 - 2087162..2087311 (-) 150 WP_167313206.1 hypothetical protein -
  A2I63_RS10215 (A2I63_10065) - 2087855..2088079 (+) 225 WP_015899727.1 hypothetical protein -
  A2I63_RS10220 - 2088072..2088218 (-) 147 WP_165380129.1 hypothetical protein -
  A2I63_RS10225 (A2I63_10070) - 2088233..2089000 (-) 768 WP_015899725.1 phage antirepressor -
  A2I63_RS10230 (A2I63_10075) - 2089037..2089264 (-) 228 WP_050731401.1 helix-turn-helix transcriptional regulator -
  A2I63_RS10235 (A2I63_10080) - 2089439..2089762 (+) 324 WP_015899724.1 helix-turn-helix transcriptional regulator -
  A2I63_RS10240 (A2I63_10085) - 2089778..2090242 (+) 465 WP_015899723.1 ImmA/IrrE family metallo-endopeptidase -
  A2I63_RS10245 (A2I63_10090) - 2090584..2090766 (+) 183 WP_015899722.1 hypothetical protein -
  A2I63_RS10250 (A2I63_10095) - 2091243..2092292 (+) 1050 WP_015899721.1 tyrosine-type recombinase/integrase -
  A2I63_RS10255 (A2I63_10100) sufB 2092360..2093757 (-) 1398 WP_015899720.1 Fe-S cluster assembly protein SufB -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15877.49 Da        Isoelectric Point: 5.2069

>NTDB_id=180545 A2I63_RS10175 WP_015899733.1 2084749..2085180(-) (ssbA) [Staphylococcus carnosus strain TMW 2.243]
MINRVVLVGRLTKDPEFRTTPSGVDVATFTLAVNRNFKSKNGEQQADFINCVVFRKQAENVNNYLNKGSLAGVDGRLQSR
SYENKEGQRVFVTEVVADSVQFLEPKNNNQQNNQPQQQQGQAPAGNNPFGNASDDISESELPF

Nucleotide


Download         Length: 432 bp        

>NTDB_id=180545 A2I63_RS10175 WP_015899733.1 2084749..2085180(-) (ssbA) [Staphylococcus carnosus strain TMW 2.243]
ATGATTAACAGAGTCGTATTAGTAGGTCGTTTAACAAAAGATCCAGAATTCAGAACAACCCCGAGCGGTGTAGATGTAGC
AACATTCACACTAGCAGTCAACCGTAATTTTAAGAGTAAAAATGGAGAGCAACAGGCGGACTTTATCAACTGTGTTGTAT
TCCGTAAACAAGCAGAAAACGTCAATAATTATTTGAATAAAGGCAGTTTAGCAGGTGTCGATGGTCGCTTACAATCACGC
AGCTATGAAAATAAGGAAGGACAACGTGTGTTTGTAACCGAAGTAGTAGCAGATAGTGTTCAATTCTTAGAGCCAAAGAA
TAACAATCAACAAAACAATCAACCTCAACAACAACAAGGGCAAGCACCAGCAGGCAATAACCCGTTTGGAAATGCTAGCG
ACGATATTTCAGAATCGGAACTCCCTTTCTAG

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9DJE5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

56.977

100

0.685
  ssb Latilactobacillus sakei subsp. sakei 23K

52.353

100

0.622
  ssbB Bacillus subtilis subsp. subtilis str. 168

60.377

74.126

0.448
  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.75

100

0.441
  ssbA Streptococcus mutans UA159

43.056

100

0.434
  ssbB/cilA Streptococcus mitis NCTC 12261

41.259

100

0.413
  ssbB/cilA Streptococcus pneumoniae TIGR4

41.259

100

0.413
  ssbB/cilA Streptococcus pneumoniae Rx1

40.559

100

0.406
  ssbB/cilA Streptococcus pneumoniae D39

40.559

100

0.406
  ssbB/cilA Streptococcus pneumoniae R6

40.559

100

0.406
  ssbB/cilA Streptococcus mitis SK321

40.559

100

0.406

Multiple sequence alignment