Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A2I66_RS12220 Genome accession   NZ_CP015535
Coordinates   2446868..2448238 (-) Length   456 a.a.
NCBI ID   WP_053464798.1    Uniprot ID   -
Organism   Staphylococcus carnosus strain TMW 2.218     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2441868..2453238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A2I66_RS12205 (A2I66_11985) cysE 2443099..2443728 (-) 630 WP_174221515.1 serine O-acetyltransferase -
  A2I66_RS12210 (A2I66_11990) gltX 2444031..2445485 (-) 1455 WP_065865799.1 glutamate--tRNA ligase -
  A2I66_RS12215 (A2I66_11995) - 2445779..2446837 (-) 1059 WP_065865800.1 PIN/TRAM domain-containing protein -
  A2I66_RS12220 (A2I66_12000) radA 2446868..2448238 (-) 1371 WP_053464798.1 DNA repair protein RadA Machinery gene
  A2I66_RS12225 (A2I66_12005) - 2448501..2450966 (-) 2466 WP_065865801.1 ATP-dependent Clp protease ATP-binding subunit -
  A2I66_RS12230 (A2I66_12010) - 2450978..2451982 (-) 1005 WP_046100590.1 protein arginine kinase -
  A2I66_RS12235 (A2I66_12015) - 2451987..2452409 (-) 423 Protein_2400 UvrB/UvrC motif-containing protein -
  A2I66_RS12240 (A2I66_12020) - 2452530..2452991 (-) 462 WP_012664206.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50281.92 Da        Isoelectric Point: 7.4017

>NTDB_id=180517 A2I66_RS12220 WP_053464798.1 2446868..2448238(-) (radA) [Staphylococcus carnosus strain TMW 2.218]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIIEKKEAPSGRGMRTREQTAKVTKLNQVQNETTPRIKTSSPEFDR
VLGGGIVQGSLVLIGGDPGIGKSTLLLQICSALSQNKKVLYITGEESLNQTKLRADRLDEDSSNLNVFAETDLEVIKEAV
KQTEPDLVVVDSIQTIYHPDINSAPGSVSQVRESTQILMGIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMKHSGLKGVKNPSEMFLEERSTNVAGSTIVPTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLNLLMAVLEKKESYLLQQQDAYIKVAGGVRLTEPAVDLALIAAIASSFKDQPVNGMDCYVGEVGLTGEV
RRVSRIEQRVQEAAKLGFKRVIIPKTNIGGWDFPEGIQVIGVTSVHEALDFALMKR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=180517 A2I66_RS12220 WP_053464798.1 2446868..2448238(-) (radA) [Staphylococcus carnosus strain TMW 2.218]
TTGGCAAAAAAGAAAGTTATTTTTGAATGTATGGCATGTGGCTATCAATCCCCTAAATGGATGGGAAAATGTCCTAATTG
TGGTGCTTGGAACCAAATGGAAGAAATTATTGAGAAAAAAGAAGCGCCAAGTGGAAGAGGCATGCGTACACGAGAACAAA
CAGCAAAAGTTACCAAGCTTAACCAAGTACAAAATGAAACTACACCTCGTATCAAAACGAGTTCCCCTGAATTCGATAGA
GTATTAGGTGGCGGTATTGTACAAGGTTCGCTTGTCTTAATTGGCGGTGATCCTGGTATTGGTAAATCAACATTGCTGTT
GCAAATCTGTTCAGCATTATCACAAAATAAAAAAGTTTTATATATAACAGGTGAGGAATCACTGAACCAAACTAAATTAC
GAGCAGACCGTTTAGATGAAGATTCAAGTAATTTGAATGTGTTTGCAGAAACGGATTTAGAAGTGATTAAAGAAGCGGTC
AAACAGACTGAACCTGATTTAGTCGTAGTAGATTCTATTCAAACAATTTATCATCCGGATATCAACTCTGCGCCGGGTTC
TGTATCGCAAGTACGAGAAAGTACGCAAATTTTAATGGGTATTGCTAAACAAATGAATATTGCGACTTTCATTGTCGGTC
ACGTGACTAAAGAAGGTCAGATTGCAGGTCCGAGATTACTAGAGCACATGGTAGATACTGTATTGTATTTCGAAGGTGAC
GAACATCATGCGTACCGTATTTTACGTGCAGTTAAAAACCGTTTTGGTTCTACCAATGAAATGGGTATTTTCGAAATGAA
ACATAGCGGTTTGAAAGGTGTTAAAAACCCATCGGAAATGTTTTTAGAAGAACGGTCTACTAATGTGGCAGGTTCTACAA
TTGTGCCGACCATGGAAGGTACACGTCCGTTATTGATTGAAGTACAAGCATTAGTAACACCTACAACTTTCAATAACCCG
AGACGAATGGCCACGGGTATTGATCATAACCGTTTAAATTTATTGATGGCTGTACTTGAGAAAAAAGAAAGTTACTTACT
GCAGCAGCAAGATGCTTATATCAAAGTAGCAGGCGGAGTGAGACTGACTGAACCAGCTGTTGACTTAGCCCTGATTGCAG
CAATCGCTTCCAGCTTTAAAGACCAACCCGTAAATGGCATGGATTGTTATGTGGGTGAGGTTGGTTTAACAGGTGAAGTG
CGTCGCGTTTCACGTATCGAACAGCGTGTACAAGAAGCCGCAAAACTTGGATTTAAACGTGTGATTATCCCTAAAACCAA
TATCGGTGGTTGGGATTTTCCTGAGGGTATTCAAGTAATTGGTGTAACGAGCGTGCACGAAGCGCTGGATTTCGCATTAA
TGAAACGCTGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(360-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(66-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.228

100

0.662
  radA Streptococcus pneumoniae Rx1

60.579

98.465

0.596
  radA Streptococcus pneumoniae D39

60.579

98.465

0.596
  radA Streptococcus pneumoniae R6

60.579

98.465

0.596
  radA Streptococcus pneumoniae TIGR4

60.579

98.465

0.596
  radA Streptococcus mitis NCTC 12261

60.579

98.465

0.596
  radA Streptococcus mitis SK321

60.579

98.465

0.596

Multiple sequence alignment