Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GFC30_RS00505 Genome accession   NZ_CP015438
Coordinates   87174..88547 (-) Length   457 a.a.
NCBI ID   WP_409978491.1    Uniprot ID   A0A161HTW1
Organism   Anoxybacteroides amylolyticum strain DSM 15939     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 82174..93547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFC30_RS00480 (GFC30_97) cysE 82303..82965 (-) 663 WP_066326984.1 serine O-acetyltransferase -
  GFC30_RS00485 (GFC30_98) gltX 83227..84717 (-) 1491 WP_066322106.1 glutamate--tRNA ligase -
  GFC30_RS00490 (GFC30_99) ispF 84788..85264 (-) 477 WP_066322108.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GFC30_RS00495 (GFC30_100) ispD 85269..85952 (-) 684 WP_066322111.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GFC30_RS00500 (GFC30_101) - 85970..87064 (-) 1095 WP_066322113.1 PIN/TRAM domain-containing protein -
  GFC30_RS00505 (GFC30_102) radA 87174..88547 (-) 1374 WP_409978491.1 DNA repair protein RadA Machinery gene
  GFC30_RS00510 (GFC30_103) clpC 88627..91059 (-) 2433 WP_066322120.1 ATP-dependent protease ATP-binding subunit ClpC -
  GFC30_RS00515 (GFC30_104) - 91056..92147 (-) 1092 WP_066322122.1 protein arginine kinase -
  GFC30_RS00520 (GFC30_105) - 92150..92692 (-) 543 WP_066322124.1 UvrB/UvrC motif-containing protein -
  GFC30_RS00525 (GFC30_106) - 92785..93246 (-) 462 WP_066322126.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49864.36 Da        Isoelectric Point: 7.8308

>NTDB_id=179751 GFC30_RS00505 WP_409978491.1 87174..88547(-) (radA) [Anoxybacteroides amylolyticum strain DSM 15939]
MKKKTKFVCQSCGYESAKWMGKCPGCQAWNTMVEEMERGKPATRGVFVHSSDTASKPVPITTITTAQEPRITTNLFEFNR
VLGGGIVKGSLVLIGGDPGIGKSTLLLQVSAQLAAQHHKVLYISGEESVKQTKLRADRLSVSAHDLYVLAETDLEHIHQA
IEEIQPHFVVIDSIQTIYRAEITSAPGSVSQVRECTGELMRIAKTKGVAIFIVGHVTKEGSIAGPRILEHMVDTVLYFEG
ERHHTYRILRAVKNRFGSTNEIGIFEMRESGLLEVENPSEVFLEERSGGVAGSTVVASMEGTRPVLVEIQALISPTSFGN
PRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDHPTNPSDVIIGEVGLTGE
VRRVSRIEQRVQEAVKLGFQRIILPKNNLGGWSAPKGVDIIGVSHVAEALEYAFSRK

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=179751 GFC30_RS00505 WP_409978491.1 87174..88547(-) (radA) [Anoxybacteroides amylolyticum strain DSM 15939]
ATGAAGAAAAAAACAAAATTCGTTTGCCAATCATGTGGATACGAATCAGCTAAGTGGATGGGGAAATGCCCAGGATGCCA
AGCGTGGAATACGATGGTGGAAGAAATGGAGCGGGGGAAACCGGCGACAAGAGGAGTGTTTGTTCATTCTTCCGACACCG
CTTCTAAACCGGTACCCATTACAACAATTACGACAGCGCAAGAGCCGAGGATTACGACAAATCTTTTCGAGTTTAATCGC
GTATTAGGTGGCGGCATTGTGAAAGGATCCCTTGTGTTAATCGGTGGAGATCCAGGGATTGGGAAATCAACGCTTCTTTT
GCAAGTCTCCGCACAGCTGGCTGCTCAACACCATAAAGTACTATATATTTCTGGAGAAGAATCGGTAAAGCAAACGAAAT
TGCGAGCGGACCGCCTCAGTGTTTCCGCGCACGACTTATACGTTTTAGCAGAAACAGATTTAGAGCACATTCATCAAGCC
ATTGAAGAGATTCAGCCGCATTTTGTTGTCATTGATTCTATCCAAACGATATATCGCGCGGAAATTACCTCTGCACCGGG
AAGCGTTTCCCAAGTAAGGGAGTGCACTGGAGAGTTGATGAGAATTGCGAAAACGAAAGGTGTGGCTATTTTCATTGTTG
GCCATGTGACGAAAGAAGGATCGATTGCTGGACCGAGAATTTTAGAGCATATGGTAGATACGGTGCTTTATTTTGAAGGA
GAAAGGCATCATACGTATCGAATTTTACGAGCGGTGAAAAACCGGTTTGGTTCGACGAACGAAATCGGCATTTTTGAAAT
GAGAGAGTCTGGGTTATTGGAAGTAGAAAATCCGTCCGAAGTATTTTTAGAAGAGCGCTCTGGCGGCGTTGCGGGCTCGA
CGGTTGTTGCTTCCATGGAAGGAACGAGACCTGTACTCGTTGAAATTCAAGCATTAATTTCACCGACAAGCTTTGGAAAT
CCACGACGAATGGCGACTGGCATTGACCATAATCGTGTGTCGTTGTTGATGGCAGTGCTTGAAAAGCGGGTCGGGCTACT
TTTACAAAATCAAGATGCGTACTTAAAAGTAGCAGGCGGTGTGAAACTCGATGAGCCAGCAATTGATTTAGCAGTTGCGG
TAAGCATTGCTTCGAGCTTCCGCGATCATCCCACGAACCCATCGGACGTAATCATTGGCGAAGTAGGGCTAACAGGGGAA
GTACGTCGCGTTTCTCGCATTGAACAGCGCGTACAAGAAGCCGTGAAGCTCGGATTTCAGCGTATTATTTTACCAAAAAA
TAATCTTGGCGGCTGGAGTGCACCAAAAGGGGTGGACATTATCGGCGTATCGCATGTTGCAGAGGCATTGGAATATGCAT
TTTCTCGAAAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(7-34)

AAA domain (AAA_25)

(79-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A161HTW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

78.194

99.344

0.777
  radA Streptococcus mitis NCTC 12261

62.996

99.344

0.626
  radA Streptococcus pneumoniae Rx1

62.996

99.344

0.626
  radA Streptococcus pneumoniae D39

62.996

99.344

0.626
  radA Streptococcus pneumoniae R6

62.996

99.344

0.626
  radA Streptococcus pneumoniae TIGR4

62.996

99.344

0.626
  radA Streptococcus mitis SK321

62.775

99.344

0.624

Multiple sequence alignment