Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GFC29_RS08240 Genome accession   NZ_CP015436
Coordinates   1710594..1711658 (-) Length   354 a.a.
NCBI ID   WP_044744511.1    Uniprot ID   -
Organism   Anoxybacillus sp. B7M1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1705594..1716658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFC29_RS08200 (GFC29_1690) - 1706628..1706807 (-) 180 WP_044744503.1 YqzE family protein -
  GFC29_RS08205 (GFC29_1691) - 1706832..1707347 (-) 516 WP_044744504.1 shikimate kinase -
  GFC29_RS08210 (GFC29_1692) comGG 1707700..1708086 (-) 387 WP_044744505.1 competence type IV pilus minor pilin ComGG -
  GFC29_RS08215 (GFC29_1693) comGF 1708083..1708541 (-) 459 WP_052660993.1 competence type IV pilus minor pilin ComGF -
  GFC29_RS08220 (GFC29_1694) comGE 1708492..1708845 (-) 354 WP_044744507.1 competence type IV pilus minor pilin ComGE -
  GFC29_RS08225 (GFC29_1695) comGD 1708829..1709275 (-) 447 WP_044744508.1 competence type IV pilus minor pilin ComGD -
  GFC29_RS08230 (GFC29_1696) comGC 1709259..1709555 (-) 297 WP_080862312.1 competence type IV pilus major pilin ComGC Machinery gene
  GFC29_RS08235 (GFC29_1697) comGB 1709573..1710601 (-) 1029 WP_044744510.1 competence type IV pilus assembly protein ComGB -
  GFC29_RS08240 (GFC29_1698) comGA 1710594..1711658 (-) 1065 WP_044744511.1 competence type IV pilus ATPase ComGA Machinery gene
  GFC29_RS08245 (GFC29_1699) - 1711900..1712604 (+) 705 WP_044744512.1 metalloregulator ArsR/SmtB family transcription factor -
  GFC29_RS08250 (GFC29_1700) - 1712749..1712991 (+) 243 WP_044744513.1 DUF2626 domain-containing protein -
  GFC29_RS08255 (GFC29_1701) - 1713225..1714352 (-) 1128 WP_044744514.1 class I SAM-dependent methyltransferase -
  GFC29_RS08260 (GFC29_1702) - 1714512..1715144 (-) 633 WP_044744691.1 MBL fold metallo-hydrolase -
  GFC29_RS08265 (GFC29_1703) - 1715231..1715653 (+) 423 WP_044744515.1 hypothetical protein -
  GFC29_RS19105 (GFC29_1704) - 1715716..1715892 (+) 177 WP_044744516.1 DUF2759 domain-containing protein -
  GFC29_RS08270 (GFC29_1705) - 1715956..1716312 (-) 357 WP_044744517.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39881.62 Da        Isoelectric Point: 8.6943

>NTDB_id=179717 GFC29_RS08240 WP_044744511.1 1710594..1711658(-) (comGA) [Anoxybacillus sp. B7M1]
MNEIEQLADRLVKEASEIGASDIHIVPRRDDALIQFRMDGALVVKGMLEKGLYERLLVYFKFLADMDIGERRRPQSGAME
ITQNGVHVSLRLSTLPTLYDESLVIRLLPHNSLLPLSHLALFPSTTRILLSLLHHSHGLLIFTGPTGSGKTTTLYTLLAA
CQKKWPRNVITLEDPVEKRIENMLQVQVNEKAGITYATGLKAILRHDPDVVIVGEIRDEETAKIAVRAALTGHLILSTMH
TKNAIGAIYRLLEFGVSWREIEQTMLAVTAQRLVELMCPFCKEACSAFCRRQRKIRRAAVYECLHGEALARAIQAARGEE
VEYRYRTLQHMMKKGVALGFLSPSVFKREMIEDA

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=179717 GFC29_RS08240 WP_044744511.1 1710594..1711658(-) (comGA) [Anoxybacillus sp. B7M1]
ATGAACGAAATTGAACAATTAGCGGATCGTCTCGTAAAAGAGGCGAGCGAAATCGGTGCCTCAGATATTCATATCGTTCC
AAGACGAGATGATGCCCTCATCCAGTTCCGCATGGATGGAGCGCTCGTTGTGAAGGGCATGCTGGAAAAGGGGTTATACG
AGCGGCTGCTTGTTTATTTTAAGTTTTTGGCGGATATGGATATTGGAGAGCGGCGTCGCCCGCAAAGCGGAGCCATGGAA
ATCACTCAAAACGGAGTTCATGTCAGCTTGCGCTTATCTACGCTTCCGACGCTTTATGATGAAAGCCTCGTGATTCGTCT
CCTTCCTCACAACTCACTTCTTCCTCTTTCACATCTTGCTTTATTTCCAAGCACAACGCGAATATTGCTGTCGCTTTTGC
ATCATTCCCACGGTTTGCTTATTTTTACGGGACCGACCGGTTCAGGAAAGACGACCACTTTATATACGCTGTTAGCAGCA
TGTCAGAAAAAGTGGCCACGCAATGTCATTACCTTGGAAGATCCTGTGGAAAAACGGATCGAAAATATGTTGCAAGTTCA
GGTCAATGAAAAGGCGGGGATCACGTATGCAACAGGGCTAAAGGCGATTTTGCGCCATGATCCCGATGTCGTGATCGTCG
GCGAAATCCGCGATGAAGAAACGGCGAAAATCGCGGTACGCGCCGCTTTGACAGGACATTTAATCTTATCGACGATGCAT
ACAAAAAATGCTATCGGTGCGATTTATCGCTTATTGGAGTTTGGCGTTTCTTGGCGGGAAATCGAACAAACGATGCTGGC
TGTGACAGCCCAACGCCTTGTTGAACTGATGTGCCCATTTTGCAAAGAAGCGTGTTCGGCTTTTTGCCGCCGGCAAAGGA
AGATCAGACGAGCGGCCGTTTACGAATGTTTGCATGGAGAGGCGCTGGCAAGAGCGATTCAAGCGGCAAGAGGAGAAGAG
GTGGAGTATCGGTATCGCACGCTGCAGCACATGATGAAAAAAGGAGTGGCGCTCGGCTTTTTATCACCTTCCGTTTTCAA
AAGAGAGATGATAGAAGATGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.02

98.588

0.562
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.21

92.938

0.364

Multiple sequence alignment