Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   A6045_RS06500 Genome accession   NZ_CP015433
Coordinates   1351141..1351590 (-) Length   149 a.a.
NCBI ID   WP_010945035.1    Uniprot ID   -
Organism   [Haemophilus] ducreyi strain GHA8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1346141..1356590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6045_RS06475 (A6045_06490) yacG 1347040..1347228 (-) 189 WP_010945040.1 DNA gyrase inhibitor YacG -
  A6045_RS06480 (A6045_06495) coaE 1347231..1347848 (-) 618 WP_010945039.1 dephospho-CoA kinase -
  A6045_RS06485 (A6045_06500) - 1347866..1348558 (-) 693 WP_041603480.1 prepilin peptidase -
  A6045_RS06490 (A6045_06505) pilC 1348558..1349751 (-) 1194 WP_010945037.1 type II secretion system F family protein Machinery gene
  A6045_RS06495 (A6045_06510) pilB 1349735..1351129 (-) 1395 WP_064083186.1 GspE/PulE family protein Machinery gene
  A6045_RS06500 (A6045_06515) pilA 1351141..1351590 (-) 450 WP_010945035.1 prepilin peptidase-dependent pilin Machinery gene
  A6045_RS06505 (A6045_06520) radA 1351784..1353163 (+) 1380 WP_010945034.1 DNA repair protein RadA -
  A6045_RS06510 (A6045_06525) - 1353230..1353445 (-) 216 WP_010945033.1 YgjV family protein -
  A6045_RS06515 (A6045_06530) - 1353567..1354052 (-) 486 WP_010945031.1 dihydrofolate reductase -
  A6045_RS06520 (A6045_06535) - 1354172..1354921 (+) 750 WP_041603673.1 TSUP family transporter -
  A6045_RS06525 (A6045_06540) nth 1354951..1355586 (+) 636 WP_010945029.1 endonuclease III -
  A6045_RS06530 (A6045_06545) fdhD 1355664..1356350 (-) 687 Protein_1264 formate dehydrogenase accessory sulfurtransferase FdhD -
  A6045_RS08810 - 1356351..1356461 (+) 111 Protein_1265 formate dehydrogenase N subunit beta transmembrane domain-containing protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16256.69 Da        Isoelectric Point: 6.9012

>NTDB_id=179633 A6045_RS06500 WP_010945035.1 1351141..1351590(-) (pilA) [[Haemophilus] ducreyi strain GHA8]
MSSLFLIKPLQKAFTLIELMIVIAIIAILATVAIPSYNSYTKKAALSELLAASSAYKTDVEICVYNLGRTDGCNNGENGI
KNDKLDKSKYVASVAVENGEITVKGKESLKDYSYTMKPELQQDGHLTWKTTCNGADTSLFPANFCSTKE

Nucleotide


Download         Length: 450 bp        

>NTDB_id=179633 A6045_RS06500 WP_010945035.1 1351141..1351590(-) (pilA) [[Haemophilus] ducreyi strain GHA8]
ATGTCATCTTTATTTTTGATTAAGCCACTACAAAAAGCGTTTACTTTAATTGAATTAATGATTGTGATTGCCATTATCGC
AATTTTAGCCACGGTTGCGATTCCTTCTTACAATAGTTATACCAAAAAAGCTGCGTTATCTGAGTTATTAGCCGCTTCAT
CAGCGTATAAAACCGATGTAGAAATATGTGTTTATAATCTTGGTAGAACGGATGGATGCAATAATGGAGAAAATGGTATA
AAAAATGATAAATTAGATAAAAGTAAATATGTTGCTTCAGTTGCGGTGGAAAATGGAGAAATTACAGTAAAAGGTAAAGA
AAGCCTTAAAGATTATAGTTACACAATGAAGCCAGAACTACAACAAGATGGGCACTTAACTTGGAAAACGACTTGTAATG
GTGCTGATACTAGCCTTTTCCCTGCTAATTTTTGTTCTACTAAAGAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-33)

Pilin (bacterial filament) (Pilin)

(43-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

59.868

100

0.611
  pilA Haemophilus influenzae Rd KW20

56.25

96.644

0.544
  pilA Haemophilus influenzae 86-028NP

57.143

93.96

0.537

Multiple sequence alignment