Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A4V07_RS09740 Genome accession   NZ_CP015408
Coordinates   2037655..2039028 (+) Length   457 a.a.
NCBI ID   WP_065533663.1    Uniprot ID   -
Organism   Limosilactobacillus reuteri strain I49     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2032655..2044028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4V07_RS09710 (A4V07_09600) - 2032807..2033556 (+) 750 WP_065533657.1 amino acid ABC transporter ATP-binding protein -
  A4V07_RS09715 (A4V07_09605) - 2033572..2034363 (+) 792 WP_065533658.1 transporter substrate-binding domain-containing protein -
  A4V07_RS09720 (A4V07_09610) - 2034594..2035934 (-) 1341 WP_065533659.1 aminopeptidase C -
  A4V07_RS09725 (A4V07_09615) rpiA 2035963..2036646 (-) 684 WP_065533660.1 ribose-5-phosphate isomerase RpiA -
  A4V07_RS09730 (A4V07_09620) - 2036658..2036954 (-) 297 WP_065533661.1 GNAT family N-acetyltransferase -
  A4V07_RS09735 (A4V07_09625) - 2037093..2037629 (+) 537 WP_065533662.1 dUTP diphosphatase -
  A4V07_RS09740 (A4V07_09630) radA 2037655..2039028 (+) 1374 WP_065533663.1 DNA repair protein RadA Machinery gene
  A4V07_RS09745 (A4V07_09635) - 2039046..2040197 (+) 1152 WP_084405225.1 PIN/TRAM domain-containing protein -
  A4V07_RS09755 (A4V07_09640) cysS 2040570..2041982 (+) 1413 WP_065533665.1 cysteine--tRNA ligase -
  A4V07_RS09760 (A4V07_09645) - 2041994..2042407 (+) 414 WP_020842889.1 Mini-ribonuclease 3 -
  A4V07_RS09765 (A4V07_09650) rlmB 2042397..2043146 (+) 750 WP_065533666.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  A4V07_RS09770 (A4V07_09870) rpmG 2043212..2043361 (+) 150 WP_003666293.1 50S ribosomal protein L33 -
  A4V07_RS09775 (A4V07_09655) secE 2043373..2043549 (+) 177 WP_003666294.1 preprotein translocase subunit SecE -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49947.18 Da        Isoelectric Point: 7.7887

>NTDB_id=179242 A4V07_RS09740 WP_065533663.1 2037655..2039028(+) (radA) [Limosilactobacillus reuteri strain I49]
MAKVRTQYVCQNCGYNSPRYLGRCPNCGEWNTLVEEQVEANSAPTKKATTTLTGLVAKPQKINEIDSTETPRVKTKLNEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSDEHHRVLYVSGEESGTQIKMRAERLKVAGDDFYVYPETNMDSIR
DTIRDLKPEYVVIDSVQTMQATDVSSAIGSVSQIREVTAQLMQIAKSNNITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMHTNGLTEVKNPSEIFLEERLHDATGSAVVVSLEGTRPILVEIQALVTPTVY
GNAQRTATGLSRNRVSLIMAVLEKRANLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSIASSYRDKGTEPTDAFVGEVGLT
GEIRRVNRIEQRVAEAEKLGFKRIFIPKNNLKGWKPSTNIQVVGVSTLKEALYLALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=179242 A4V07_RS09740 WP_065533663.1 2037655..2039028(+) (radA) [Limosilactobacillus reuteri strain I49]
ATGGCAAAAGTTCGAACACAATATGTTTGCCAAAACTGTGGTTACAACTCACCACGCTACTTAGGCCGGTGTCCAAACTG
TGGTGAGTGGAATACTTTAGTAGAAGAACAGGTAGAAGCCAATTCTGCACCAACAAAAAAAGCAACCACCACCTTAACCG
GCTTAGTTGCCAAACCACAGAAAATTAATGAGATTGATAGTACAGAGACTCCCCGCGTAAAAACAAAATTGAACGAGTTA
AATCGCGTTTTAGGTGGGGGAATCGTTCCCGGATCACTAATTCTAATTGGTGGGGATCCGGGAATCGGGAAATCTACCCT
CCTTTTACAAGTTTCTGGACAATTGAGCGATGAGCATCACCGGGTTTTATATGTGTCTGGAGAAGAAAGTGGGACACAAA
TTAAAATGCGGGCGGAACGATTAAAAGTTGCTGGGGATGACTTTTATGTGTATCCAGAAACGAATATGGATAGTATTCGG
GATACTATTCGTGACCTCAAGCCAGAGTATGTTGTGATTGACTCGGTACAAACGATGCAGGCGACTGATGTAAGTTCAGC
AATTGGTAGTGTATCGCAAATTCGTGAAGTTACCGCCCAGTTAATGCAAATTGCTAAAAGTAATAACATTACGATTTTTG
TCGTTGGACATGTGACAAAGGGTGGTGCAATCGCCGGTCCAAAGATTTTAGAGCACATGGTTGATACGGTTTTGTATTTT
GAAGGAGACCTGCACCATACATATCGTATTTTGCGATCAGTGAAAAATCGATTTGGTTCAACCAACGAGCTCGGCATTTT
TGAGATGCATACAAATGGGCTGACGGAAGTAAAAAATCCATCAGAAATCTTTTTAGAGGAACGATTACATGATGCAACCG
GTTCAGCGGTGGTTGTTTCATTAGAAGGAACGCGACCGATTTTAGTTGAAATTCAGGCGCTGGTAACACCGACCGTTTAT
GGAAATGCACAGCGAACTGCTACCGGTTTGAGCCGAAACCGAGTATCATTGATAATGGCGGTATTAGAGAAACGTGCTAA
CCTAATGCTTCAAAATCAAGATGCTTACTTAAAAGCAGCTGGGGGCGTTAAGTTAGATGAGCCAGCAATTGATTTGGCAA
TTGCGGTAAGTATTGCCTCCAGTTATCGGGATAAAGGAACCGAGCCCACCGATGCATTTGTCGGTGAAGTAGGTTTGACT
GGGGAAATTCGCCGGGTAAACCGAATTGAACAGCGGGTTGCTGAAGCAGAAAAACTTGGCTTTAAACGAATCTTTATTCC
CAAAAATAATTTAAAGGGTTGGAAACCGTCAACTAATATTCAAGTAGTCGGTGTCTCAACCCTGAAAGAGGCGTTATATT
TAGCGCTGGGGTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(65-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.372

98.906

0.656
  radA Streptococcus mitis NCTC 12261

66.372

98.906

0.656
  radA Streptococcus pneumoniae Rx1

66.15

98.906

0.654
  radA Streptococcus pneumoniae D39

66.15

98.906

0.654
  radA Streptococcus pneumoniae R6

66.15

98.906

0.654
  radA Streptococcus pneumoniae TIGR4

66.15

98.906

0.654
  radA Bacillus subtilis subsp. subtilis str. 168

62.801

100

0.628

Multiple sequence alignment