Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BWI84_RS24805 Genome accession   NZ_CP019250
Coordinates   4785275..4785715 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain 13KWH46     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4780275..4790715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWI84_RS24780 (BWI84_24785) aroP 4781273..4782646 (+) 1374 WP_000969914.1 aromatic amino acid transporter AroP -
  BWI84_RS24785 (BWI84_24790) ampE 4782689..4783543 (-) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  BWI84_RS24790 (BWI84_24795) ampD 4783540..4784091 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BWI84_RS24795 (BWI84_24800) nadC 4784179..4785072 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BWI84_RS24805 (BWI84_24810) pilA 4785275..4785715 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  BWI84_RS24810 (BWI84_24815) pilB 4785725..4787110 (+) 1386 WP_001470984.1 type II secretion system protein GspE Machinery gene
  BWI84_RS24815 (BWI84_24820) hofC 4787100..4788302 (+) 1203 WP_000157234.1 protein transport protein HofC -
  BWI84_RS24820 (BWI84_24825) guaC 4788337..4789380 (-) 1044 WP_001217338.1 GMP reductase -
  BWI84_RS29110 - 4789536..4789580 (-) 45 WP_120795372.1 protein YacM -
  BWI84_RS24825 (BWI84_24830) coaE 4789605..4790225 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=179232 BWI84_RS24805 WP_000360895.1 4785275..4785715(+) (pilA) [Escherichia coli strain 13KWH46]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=179232 BWI84_RS24805 WP_000360895.1 4785275..4785715(+) (pilA) [Escherichia coli strain 13KWH46]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363