Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   BWI82_RS14925 Genome accession   NZ_CP019243
Coordinates   2898155..2898823 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain Combat2C1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2893155..2903823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWI82_RS14895 (BWI82_14905) rfaQ 2893333..2894391 (-) 1059 WP_000360397.1 lipopolysaccharide core heptosyltransferase RfaQ -
  BWI82_RS14900 (BWI82_14910) waaA 2894804..2896081 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  BWI82_RS14905 (BWI82_14915) coaD 2896089..2896568 (+) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  BWI82_RS14910 (BWI82_14920) mutM 2896607..2897416 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  BWI82_RS14915 (BWI82_14925) rpmG 2897514..2897681 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  BWI82_RS14920 (BWI82_14930) rpmB 2897702..2897938 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  BWI82_RS14925 (BWI82_14935) radC 2898155..2898823 (-) 669 WP_001297375.1 RadC family protein Machinery gene
  BWI82_RS14930 (BWI82_14940) coaBC 2898995..2900215 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  BWI82_RS14935 (BWI82_14945) dut 2900193..2900651 (+) 459 WP_000976070.1 dUTP diphosphatase -
  BWI82_RS14940 (BWI82_14950) slmA 2900758..2901354 (+) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  BWI82_RS14945 (BWI82_14955) pyrE 2901391..2902032 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  BWI82_RS14950 (BWI82_14960) rph 2902098..2902814 (-) 717 WP_001247094.1 ribonuclease PH -
  BWI82_RS14960 (BWI82_14970) yicC 2902941..2903804 (+) 864 WP_000621336.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=179034 BWI82_RS14925 WP_001297375.1 2898155..2898823(-) (radC) [Escherichia coli strain Combat2C1]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=179034 BWI82_RS14925 WP_001297375.1 2898155..2898823(-) (radC) [Escherichia coli strain Combat2C1]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GTTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGAG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496