Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   AS891_RS10465 Genome accession   NZ_CP015375
Coordinates   2002425..2003192 (+) Length   255 a.a.
NCBI ID   WP_003230055.1    Uniprot ID   A0AAE2SJ05
Organism   Bacillus subtilis subsp. subtilis strain KCTC 3135     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1997425..2008192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS891_RS10435 (AS891_10430) pgpH 1997721..1999856 (+) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -
  AS891_RS10440 (AS891_10435) ybeY 1999857..2000330 (+) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  AS891_RS10445 (AS891_10440) unpK 2000311..2000682 (+) 372 WP_004398824.1 diacylglycerol kinase family protein -
  AS891_RS10450 (AS891_10445) - 2000801..2001211 (+) 411 WP_003230049.1 cytidine deaminase -
  AS891_RS10455 (AS891_10450) era 2001192..2002097 (+) 906 WP_003230051.1 GTPase Era -
  AS891_RS10460 (AS891_10455) yqzL 2002245..2002388 (+) 144 WP_003230053.1 YqzL family protein -
  AS891_RS10465 (AS891_10460) recO 2002425..2003192 (+) 768 WP_003230055.1 DNA repair protein RecO Machinery gene
  AS891_RS10470 (AS891_10465) glyQ 2003489..2004376 (+) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  AS891_RS10475 (AS891_10470) glyS 2004369..2006408 (+) 2040 WP_003230059.1 glycine--tRNA ligase subunit beta -
  AS891_RS10480 (AS891_10475) ccpN 2006541..2007179 (+) 639 WP_003237058.1 transcriptional regulator CcpN -
  AS891_RS10485 (AS891_10480) ppsR 2007205..2008017 (+) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29328.02 Da        Isoelectric Point: 8.3080

>NTDB_id=178912 AS891_RS10465 WP_003230055.1 2002425..2003192(+) (recO) [Bacillus subtilis subsp. subtilis strain KCTC 3135]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=178912 AS891_RS10465 WP_003230055.1 2002425..2003192(+) (recO) [Bacillus subtilis subsp. subtilis strain KCTC 3135]
ATGCTGACAAAATGTGAAGGGATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATGTTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGTTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGGCTCTTTTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment