Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AS891_RS00040 Genome accession   NZ_CP015375
Coordinates   23893..24342 (+) Length   149 a.a.
NCBI ID   WP_003246436.1    Uniprot ID   A0AAE2SFQ0
Organism   Bacillus subtilis subsp. subtilis strain KCTC 3135     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 18893..29342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS891_RS00020 (AS891_00020) hxlR 20103..20465 (-) 363 WP_003246265.1 transcriptional activator HxlR -
  AS891_RS00025 (AS891_00025) hxlA 20698..21330 (+) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  AS891_RS00030 (AS891_00030) hxlB 21336..21893 (+) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  AS891_RS00035 (AS891_00035) tlpC 22004..23725 (+) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  AS891_RS00040 (AS891_00040) nucA/comI 23893..24342 (+) 450 WP_003246436.1 DNA-entry nuclease Machinery gene
  AS891_RS00045 (AS891_00045) nin/comJ 24369..24767 (+) 399 WP_003246342.1 DNA-entry nuclease inhibitor Regulator
  AS891_RS00050 (AS891_00050) bglC 24804..26237 (-) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  AS891_RS00055 (AS891_00055) yckD 26391..26723 (-) 333 WP_003246247.1 YckD family protein -
  AS891_RS00060 (AS891_00060) yckC 26805..27260 (-) 456 WP_003246356.1 RDD family protein -
  AS891_RS22695 - 27279..27476 (-) 198 WP_119122841.1 hypothetical protein -
  AS891_RS00070 (AS891_00070) yckB 27638..28501 (+) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  AS891_RS00075 (AS891_00075) yckA 28511..29191 (+) 681 WP_003234624.1 amino acid ABC transporter permease -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16486.48 Da        Isoelectric Point: 4.6274

>NTDB_id=178873 AS891_RS00040 WP_003246436.1 23893..24342(+) (nucA/comI) [Bacillus subtilis subsp. subtilis strain KCTC 3135]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=178873 AS891_RS00040 WP_003246436.1 23893..24342(+) (nucA/comI) [Bacillus subtilis subsp. subtilis strain KCTC 3135]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
GGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment