Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BT246_RS00530 Genome accession   NZ_CP015350
Coordinates   97536..98912 (+) Length   458 a.a.
NCBI ID   WP_065481457.1    Uniprot ID   A0A9W3WY03
Organism   Bacillus thuringiensis strain MYBT18246     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92536..103912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BT246_RS00510 (BT246_01030) ctsR 92733..93194 (+) 462 WP_001244560.1 transcriptional regulator CtsR -
  BT246_RS00515 (BT246_01040) - 93365..93913 (+) 549 WP_000128385.1 UvrB/UvrC motif-containing protein -
  BT246_RS00520 (BT246_01050) - 93918..94982 (+) 1065 WP_065481455.1 protein arginine kinase -
  BT246_RS00525 (BT246_01060) clpC 95005..97440 (+) 2436 WP_000971170.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BT246_RS00530 (BT246_01070) radA 97536..98912 (+) 1377 WP_065481457.1 DNA repair protein RadA Machinery gene
  BT246_RS00535 (BT246_01080) disA 98916..99989 (+) 1074 WP_000392164.1 DNA integrity scanning diadenylate cyclase DisA -
  BT246_RS00540 (BT246_01090) - 100150..101253 (+) 1104 WP_065481459.1 PIN/TRAM domain-containing protein -
  BT246_RS00545 (BT246_01100) ispD 101270..101950 (+) 681 WP_000288307.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BT246_RS00550 (BT246_01110) ispF 102067..102543 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49869.51 Da        Isoelectric Point: 6.9053

>NTDB_id=178751 BT246_RS00530 WP_065481457.1 97536..98912(+) (radA) [Bacillus thuringiensis strain MYBT18246]
MAKKKTKFICQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETEFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEETNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRVIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=178751 BT246_RS00530 WP_065481457.1 97536..98912(+) (radA) [Bacillus thuringiensis strain MYBT18246]
ATGGCTAAAAAGAAAACGAAATTTATATGTCAAGAATGTGGTTATCAGTCACCAAAATATATGGGTAAATGTCCTGGATG
TGGTCAATGGAATACACTTGTTGAAGAAATGGAGCCAGTTGTATCATCTAGGCGCCTTAATTACGCGAATGCAATTCAAA
CGGAAGTAACAAAACCAAGACGTCTTACCGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACGGAATTTCAAGAGTTT
AATCGTGTGCTTGGTGGTGGAATTGTAGATGGGTCCTTAGTGCTTATTGGTGGAGACCCTGGGATTGGAAAATCAACATT
ACTATTACAGATTTCATCACAATTAGCAGATTCTTCATATGATGTATTATACATATCGGGTGAGGAGTCGGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGCAATCTATTTGTTGTAGCAGAGACAGACCTGCAGCGTATTGCA
GCACATATTGAAGAGACGAATCCAGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAAGTGACGTCAGC
ACCAGGAAGTGTGGCACAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACAAAAGGAATCCCTATTTTCA
TTGTCGGGCATGTGACAAAAGAAGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACGTATCGTATTTTAAGGGCTGTGAAGAACCGTTTTGGTTCCACGAATGAAATGGGTATCTT
TGAAATGAAAGAGCTTGGTCTTGCGGAAGTTTTAAATCCTTCTGAAATCTTCCTTGAGGAAAGGCCGGTTGGTGTTGCAG
GATCAACAGTAGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGTGTTTCGCTTATTATGGCAGTATTAGAAAAAAGAACAGG
TTTACTATTGCAAAATCAAGATGCATATTTAAAAGTAGCTGGTGGTTTGAAATTAGACGAACCGGCAATTGATTTAGCAG
TGGCTTTAAGTATAGCCTCAAGTTTTAGAGATAAATCTACGGCACCAACTGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCGGCTAAATTAGGATTTCAACGTGTTATTATTCC
TAGAAAAAACTTAGGAGGATGGACAATTCCGGATGGGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.238

98.908

0.635
  radA Streptococcus pneumoniae D39

64.238

98.908

0.635
  radA Streptococcus pneumoniae R6

64.238

98.908

0.635
  radA Streptococcus pneumoniae TIGR4

64.238

98.908

0.635
  radA Streptococcus mitis SK321

64.238

98.908

0.635
  radA Streptococcus mitis NCTC 12261

64.018

98.908

0.633

Multiple sequence alignment