Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   BTT_RS27740 Genome accession   NZ_CP015310
Coordinates   5346655..5348004 (-) Length   449 a.a.
NCBI ID   WP_002083580.1    Uniprot ID   -
Organism   Bacillus thuringiensis serovar morrisoni str. 4AA1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5341655..5353004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTT_RS27705 (BTT_55740) - 5342299..5342523 (-) 225 Protein_5405 hypothetical protein -
  BTT_RS27710 (BTT_55750) - 5342510..5343298 (-) 789 WP_000120041.1 hypothetical protein -
  BTT_RS27715 (BTT_55760) - 5343330..5343899 (-) 570 WP_000767072.1 hypothetical protein -
  BTT_RS27720 (BTT_55770) - 5344017..5344490 (-) 474 WP_000023375.1 hypothetical protein -
  BTT_RS27725 (BTT_55780) raiA 5344763..5345305 (-) 543 WP_000671187.1 ribosome-associated translation inhibitor RaiA -
  BTT_RS27730 (BTT_55790) cspC 5345627..5345824 (-) 198 WP_001990088.1 cold shock protein CspC -
  BTT_RS27735 (BTT_55800) - 5345951..5346655 (-) 705 WP_042596636.1 ComF family protein -
  BTT_RS27740 (BTT_55810) comFA 5346655..5348004 (-) 1350 WP_002083580.1 ATP-dependent helicase ComFA Machinery gene
  BTT_RS27745 (BTT_55820) - 5348132..5349559 (-) 1428 WP_000499487.1 C40 family peptidase -
  BTT_RS27750 (BTT_55830) - 5349708..5350022 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  BTT_RS27755 (BTT_55840) - 5350195..5351037 (-) 843 WP_000684725.1 DegV family protein -
  BTT_RS27760 (BTT_55850) - 5351274..5351909 (+) 636 WP_000926669.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51166.05 Da        Isoelectric Point: 10.0230

>NTDB_id=178456 BTT_RS27740 WP_002083580.1 5346655..5348004(-) (comFA) [Bacillus thuringiensis serovar morrisoni str. 4AA1]
MLAGKQLLLDELSSDLQRELNDLKRKGEVICVQGVKKKNSKYMCQRCGNVDQRLFASFLCRRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKKLNLLQWNGKLSTGQNLAAQGVVEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKRKFRKGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLIHKRIPRVLLQWLQTYLNKKHPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=178456 BTT_RS27740 WP_002083580.1 5346655..5348004(-) (comFA) [Bacillus thuringiensis serovar morrisoni str. 4AA1]
ATGCTGGCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAGGAAGGG
AGAGGTCATATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCAGCGGC
TATTTGCGTCGTTTTTATGTAGAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAAATTAAACTTGTTACAGTGGAACGGGAAGTT
GTCTACTGGTCAGAATTTGGCGGCACAAGGTGTTGTAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTGCGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGGGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAAAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAACATCCTATTTTTTTA
TTTGTCCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAATCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATCCGGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTAGCGGTTTTAGGGGCTGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCCAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(311-408)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.131

98.441

0.503
  comFA Latilactobacillus sakei subsp. sakei 23K

41.709

88.641

0.37

Multiple sequence alignment