Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   BTI679_RS05760 Genome accession   NZ_CP015257
Coordinates   1115631..1119356 (+) Length   1241 a.a.
NCBI ID   WP_098101810.1    Uniprot ID   A0A2A8BU35
Organism   Bacillus wiedmannii strain Bt18679     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1110631..1124356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTI679_RS05745 (BTI679_11520) - 1110789..1111382 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  BTI679_RS05750 (BTI679_11530) lepB 1111439..1112002 (+) 564 WP_064476841.1 signal peptidase I -
  BTI679_RS05755 (BTI679_11540) addB 1112119..1115634 (+) 3516 WP_103655892.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BTI679_RS05760 (BTI679_11550) addA 1115631..1119356 (+) 3726 WP_098101810.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BTI679_RS05765 (BTI679_11560) - 1119369..1119656 (+) 288 WP_064476838.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  BTI679_RS05770 (BTI679_11570) gerPF 1119736..1119951 (-) 216 WP_001141570.1 spore germination protein GerPF -
  BTI679_RS05775 (BTI679_11580) - 1119994..1120380 (-) 387 WP_000902339.1 spore germination protein GerPE -
  BTI679_RS05780 (BTI679_11590) gerPD 1120396..1120590 (-) 195 WP_001102341.1 spore germination protein GerPD -
  BTI679_RS05785 (BTI679_11600) - 1120597..1121211 (-) 615 WP_103655891.1 spore germination protein GerPC -
  BTI679_RS05790 (BTI679_11610) gerPB 1121280..1121486 (-) 207 WP_048559586.1 spore germination protein GerPB -
  BTI679_RS05795 (BTI679_11620) gerPA 1121501..1121722 (-) 222 WP_001111187.1 spore germination protein GerPA -
  BTI679_RS05800 (BTI679_11630) - 1121817..1121996 (-) 180 WP_000462847.1 aspartyl-phosphate phosphatase Spo0E family protein -
  BTI679_RS05805 (BTI679_11640) - 1122243..1123142 (+) 900 WP_154131398.1 fumarylacetoacetate hydrolase family protein -
  BTI679_RS05810 (BTI679_11650) - 1123177..1123461 (-) 285 WP_046956998.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142592.83 Da        Isoelectric Point: 4.8458

>NTDB_id=178111 BTI679_RS05760 WP_098101810.1 1115631..1119356(+) (addA) [Bacillus wiedmannii strain Bt18679]
MIGNWPKKPVGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNGIFFELVDCYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEAHI
RKATELAMLPDGPAPRVETLQADAVLLGTLSSSACESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQTMKRDKGMVDFTDLEHFCLQILSEQNENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVPEGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLQWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYGYDTSWK
VEVVDGKTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKNDETILVQGVIDCMIEEEDGITLIDFKTDTIEGRFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=178111 BTI679_RS05760 WP_098101810.1 1115631..1119356(+) (addA) [Bacillus wiedmannii strain Bt18679]
ATGATTGGAAATTGGCCTAAAAAACCAGTAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATATTAGTCGCGGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGCTAGAAAAAGTATTAATTGATGAGCCAGGCTCGCAGCACGTAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCTATTTCGACAATCCATTCATTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTGGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAACTGTTAAAAGAAGAAGTATTAGATGACATATTAGAAGAAGAGTACGGAATTGAA
GATAATGGTATTTTCTTTGAACTCGTTGATTGTTATACGAGTGACCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCAGAGGCACATATT
CGTAAAGCGACGGAACTTGCAATGCTTCCTGACGGTCCGGCGCCCCGCGTTGAAACCCTGCAAGCAGATGCAGTTTTACT
TGGAACGTTATCATCATCAGCTTGTGAGTCATGGACAAGTGTATATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCACTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGTAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTTCAAACGATGAAGCGAGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAATGAAAACGGTGAAATGAATCCATCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTTGATGAATATCAAGATACGAACTTTGTGCAGGAATCCATTATTAAATTTGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTAGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGCCTGTTCCTAGGGAAGTATAAACGCTTCACACAAGAAGGATTAGACGGTGGAATGAAGATAGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGATTATGATGCTGATGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGTA
TTCAGCAAACAGAAGAAGAAGTGCCAGAAGGTGAAGAAGGTGCAGAAGTAGAAAAAGCACAACTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTACTTCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTGATCGATAAT
CCGATGCAAGATATCCCGCTTGCCGCCGTACTTCGTTCACCAATCGTTGGACTAAATGATGAAGAACTTGCGACACTTCG
TGCTCACGGAAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAGGAACAAGAAT
TACATGATAAATTACAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGTCAACAGTCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTCGGCGGTTTACCAGCCGGAAAGCAAAGGCAAGCAAACTT
ACGTGTACTTTATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGTTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGAACAGCGAGAGCTTTAGGTGAACAAGAAGATGTGGTTCGTATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTAGCGGGACTAGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTGTTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATTGGAACGGTTAAAGATGCCAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCGGGTGAAATTTATGGATATGACACGAGCTGGAAA
GTAGAAGTTGTTGACGGCAAAACGTTACTTGCACCGGAACCGGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAAAAAAAAGCTGTTCCGCTACAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTGATGTGGAAGTACGGATATGAGG
AAGCAACGTCTCACCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAACTACCAATCAGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTGCGTGCACCAATTAAAACACGCCCTCGTTTTATGGAGAAAAAAGGATTAACGTATGCTGAAAGAGG
TACGGCAGTTCACGCTGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGAGAGCAAATTGCCG
GAATGGTAAATAAGGAATTATTAACATTCGAACAAGCTGAAGAAATAGCAATTGAAAAAGTCATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAGGC
GTATCAAGATTGGCAAGGTAAGAATGACGAAACAATACTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GTATCACTTTAATTGATTTTAAAACGGATACGATTGAAGGAAGGTTCCCAGGTGGATTCGATCAAGCAAAACCAATTTTA
GAAGATCGATACAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAAAAAAGCTTGCAACATCCCGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAATCATGTTGTGAAAATTGAAGAATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A8BU35

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.007

100

0.533

Multiple sequence alignment