Detailed information NTG0178095

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: BTI247_RS27030
• Genome accession: NZ_CP015250
• Coordinates: 5283329..5284678 (-)
• Length: 449 a.a.
• NCBI ID: WP_069356508.1
• Uniprot ID: A0A9W3SYM6
• Organism: Bacillus thuringiensis Bt18247
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5278329..5289678

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BTI247_RS27010 (BTI247_55370)	secA	5278654..5281161 (-)	2508	WP_069356506.1	preprotein translocase subunit SecA	-
BTI247_RS27015 (BTI247_55380)	raiA	5281435..5281977 (-)	543	WP_000671189.1	ribosome-associated translation inhibitor RaiA	-
BTI247_RS27020 (BTI247_55390)	cspC	5282301..5282498 (-)	198	WP_001990088.1	cold shock protein CspC	-
BTI247_RS27025 (BTI247_55400)	-	5282625..5283329 (-)	705	WP_084746290.1	ComF family protein	-
BTI247_RS27030 (BTI247_55410)	comFA	5283329..5284678 (-)	1350	WP_069356508.1	ATP-dependent helicase ComFA	Machinery gene
BTI247_RS27035 (BTI247_55420)	-	5284806..5286233 (-)	1428	WP_000499486.1	C40 family peptidase	-
BTI247_RS27040 (BTI247_55430)	-	5286382..5286696 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
BTI247_RS27045 (BTI247_55440)	-	5286869..5287711 (-)	843	WP_000684727.1	DegV family protein	-
BTI247_RS27050 (BTI247_55450)	-	5287948..5288583 (+)	636	WP_000926670.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51165.03 Da
• Isoelectric Point: 10.0316

>NTDB_id=178095 BTI247_RS27030 WP_069356508.1 5283329..5284678(-) (comFA) [Bacillus thuringiensis Bt18247]
MLAGKQLLLDELSSDLQRELNDLKKKGEVVCVQGVKKKASKYVCQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECALLVRGIAERKREKNLNLLRWSGTLSTGQNLAAQGVIEAIKRKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKRKFRRGEQKGVIVSGRYHRHPLPVPLFYWCGNWKKSLIHKRIPRVLLQWLQTYLNKKYPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVVYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=178095 BTI247_RS27030 WP_069356508.1 5283329..5284678(-) (comFA) [Bacillus thuringiensis Bt18247]
ATGCTAGCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAAGAAGGG
AGAGGTCGTCTGTGTACAAGGTGTAAAAAAGAAGGCTTCTAAATATGTATGTCAGCGCTGTGGAAATATAGAACAGCGGC
TATTTGCGTCGTTTTTATGTAAAAGATGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTTTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTTAAACTTGCTACGGTGGAGCGGAACGTT
GTCTACTGGTCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCGAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAGGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCAACCACGCATCAATTATTGCGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAGAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTGCCAGTTCCTTTGTTTTATTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACGTACTTAAATAAAAAATATCCTATTTTTTTA
TTTGTTCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAGTCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATCCGGGTAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACAGTAAAAAATTTGCAAGTAGCGGTTTTAGGGGCGGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCGTTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (108-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	50.679	98.441	0.499
comFA	Latilactobacillus sakei subsp. sakei 23K	41.709	88.641	0.37