Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BTI247_RS22240 Genome accession   NZ_CP015250
Coordinates   4384908..4385654 (-) Length   248 a.a.
NCBI ID   WP_000487009.1    Uniprot ID   -
Organism   Bacillus thuringiensis Bt18247     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4379908..4390654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTI247_RS22225 (BTI247_45700) dnaG 4380669..4382465 (-) 1797 WP_069356206.1 DNA primase -
  BTI247_RS33875 - 4382547..4382690 (-) 144 WP_000479733.1 hypothetical protein -
  BTI247_RS34405 - 4382827..4382958 (-) 132 WP_002024806.1 hypothetical protein -
  BTI247_RS22230 (BTI247_45710) - 4383326..4384138 (-) 813 WP_000368944.1 pyruvate, water dikinase regulatory protein -
  BTI247_RS22235 (BTI247_45720) - 4384168..4384800 (-) 633 WP_000583756.1 helix-turn-helix transcriptional regulator -
  BTI247_RS22240 (BTI247_45730) recO 4384908..4385654 (-) 747 WP_000487009.1 DNA repair protein RecO Machinery gene
  BTI247_RS22245 (BTI247_45740) - 4385688..4385831 (-) 144 WP_000883924.1 YqzL family protein -
  BTI247_RS22250 (BTI247_45750) era 4386008..4386913 (-) 906 WP_001080230.1 GTPase Era -
  BTI247_RS22255 (BTI247_45760) - 4386906..4387304 (-) 399 WP_001086285.1 cytidine deaminase -
  BTI247_RS22260 (BTI247_45770) - 4387404..4387757 (-) 354 WP_069356207.1 diacylglycerol kinase family protein -
  BTI247_RS22265 (BTI247_45780) ybeY 4387754..4388224 (-) 471 WP_000054684.1 rRNA maturation RNase YbeY -
  BTI247_RS22270 (BTI247_45790) - 4388221..4390365 (-) 2145 WP_069356208.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28618.21 Da        Isoelectric Point: 8.0566

>NTDB_id=178085 BTI247_RS22240 WP_000487009.1 4384908..4385654(-) (recO) [Bacillus thuringiensis Bt18247]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=178085 BTI247_RS22240 WP_000487009.1 4384908..4385654(-) (recO) [Bacillus thuringiensis Bt18247]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAACTTGGTAAGGTAAGTGCAATGGCAAGAGGGGCAAAAAAACCGAAAAGCCGATTAGCATCTGTGTCGCAATTAATGA
CACATGGCCATTTTCTTATTCAAATGGGATCTGGGCTTGGAACATTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAC
TTTGTCGCCTTCTCCGTAAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCAGAGCAAGACCCGTATCGTATACCGGTAGG
AGAAGCTGTTCATAAATTATTACGTCTTTTCTTCCATTTCGATTTACATAGGCTCGGAAATGTATCGGTAAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTTTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment