Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   BE948_RS25970 Genome accession   NZ_CP019005
Coordinates   4550404..4551063 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain Ecol_AZ155     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4545404..4556063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BE948_RS25945 (BE948_26660) plsC 4545550..4546287 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  BE948_RS25950 (BE948_26670) parC 4546521..4548779 (-) 2259 WP_001281842.1 DNA topoisomerase IV subunit A -
  BE948_RS25960 (BE948_26680) ygiV 4549325..4549807 (-) 483 WP_000183494.1 GyrI-like domain-containing protein -
  BE948_RS25965 (BE948_26685) ygiW 4549860..4550252 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  BE948_RS25970 (BE948_26690) ciaR 4550404..4551063 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  BE948_RS25975 (BE948_26695) qseC 4551060..4552409 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  BE948_RS25980 (BE948_26700) ygiZ 4552455..4552787 (-) 333 WP_000912120.1 DUF2645 family protein -
  BE948_RS25985 (BE948_26705) mdaB 4553106..4553687 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  BE948_RS25990 (BE948_26710) ygiN 4553718..4554032 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  BE948_RS25995 (BE948_26715) parE 4554080..4555972 (-) 1893 WP_000195298.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=177907 BE948_RS25970 WP_001221502.1 4550404..4551063(+) (ciaR) [Escherichia coli strain Ecol_AZ155]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=177907 BE948_RS25970 WP_001221502.1 4550404..4551063(+) (ciaR) [Escherichia coli strain Ecol_AZ155]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment