Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A2J09_RS18235 Genome accession   NZ_CP015224
Coordinates   3692024..3693400 (-) Length   458 a.a.
NCBI ID   WP_012296093.1    Uniprot ID   W7RHU4
Organism   Lysinibacillus sphaericus strain 2362     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3687024..3698400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A2J09_RS18215 (A2J09_18210) gltX 3687645..3689105 (-) 1461 WP_012296090.1 glutamate--tRNA ligase -
  A2J09_RS18220 (A2J09_18215) ispF 3689195..3689671 (-) 477 WP_008180601.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  A2J09_RS18225 (A2J09_18220) ispD 3690015..3690710 (-) 696 WP_012296091.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  A2J09_RS18230 (A2J09_18225) - 3690722..3691807 (-) 1086 WP_012296092.1 PIN/TRAM domain-containing protein -
  A2J09_RS18235 (A2J09_18230) radA 3692024..3693400 (-) 1377 WP_012296093.1 DNA repair protein RadA Machinery gene
  A2J09_RS18240 (A2J09_18235) - 3693492..3695936 (-) 2445 WP_031416732.1 ATP-dependent Clp protease ATP-binding subunit -
  A2J09_RS18245 (A2J09_18240) - 3695926..3697035 (-) 1110 WP_012296095.1 protein arginine kinase -
  A2J09_RS18250 (A2J09_18245) - 3697032..3697574 (-) 543 WP_031416733.1 UvrB/UvrC motif-containing protein -
  A2J09_RS18255 (A2J09_18250) - 3697588..3698058 (-) 471 WP_031416734.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50138.99 Da        Isoelectric Point: 7.3824

>NTDB_id=177718 A2J09_RS18235 WP_012296093.1 3692024..3693400(-) (radA) [Lysinibacillus sphaericus strain 2362]
MAKKKTKFVCSGCGYESAKWMGRCPGCGEWNKMVEEVEVIAKGPRGAFQHSATVTQKAIPIIQVEASEESRVATEMGELN
RVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSALLSNKGHRVLYISGEESIRQTKLRAERLGVISQELYIYSETNLEFLNQ
TIEEVQPKFVIVDSIQTVFHPEVTSAPGSVSQVRECTAELMRIAKTKGIAIFLVGHVTKEGQIAGPRILEHMVDTVLYFE
GERHHNHRILRSQKNRFGSTNEIAIFEMLQGGLKEVLNPSELFLQERSQGAAGSTIVASMEGTRPILVEIQSLVTPTSFN
YPKRMATGVDQNRVQLLMAVLEKRMGLMLQAQDAYIKVAGGVKLDEPAIDLAVLTSIVSSFKDQAVRATDCFIGEVGLTG
EVRRVSRIEQRVIEAAKLGFKKVFIPASNIGGWDFPQGIEIVGVETIKDALNACFREL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=177718 A2J09_RS18235 WP_012296093.1 3692024..3693400(-) (radA) [Lysinibacillus sphaericus strain 2362]
TTGGCAAAGAAAAAAACGAAATTTGTATGTTCAGGCTGTGGCTATGAATCGGCAAAATGGATGGGAAGATGTCCAGGCTG
TGGAGAATGGAATAAAATGGTGGAGGAAGTAGAAGTTATAGCGAAAGGACCAAGAGGGGCATTCCAGCATTCTGCTACAG
TTACTCAAAAGGCAATCCCCATTATTCAGGTCGAAGCTAGTGAAGAGTCTCGAGTGGCGACAGAAATGGGCGAGCTGAAT
CGTGTATTAGGCGGTGGTATTGTGCCTGGCTCTCTAGTATTAATAGGTGGCGATCCTGGGATTGGGAAATCAACATTATT
ACTACAAGTTTCAGCTTTGCTCTCAAATAAAGGCCATCGCGTGCTGTATATTTCAGGTGAGGAGTCTATTCGTCAAACGA
AGCTACGAGCAGAGCGTTTAGGGGTAATCTCTCAAGAGCTGTACATTTATTCCGAAACGAATCTTGAGTTTTTGAATCAA
ACGATTGAGGAAGTACAGCCGAAATTTGTCATTGTTGATTCCATACAAACGGTCTTTCATCCAGAGGTAACAAGTGCGCC
AGGTAGTGTATCTCAGGTACGTGAGTGTACAGCTGAGCTTATGCGAATAGCTAAAACAAAAGGGATTGCTATATTCTTAG
TTGGGCATGTTACGAAAGAGGGGCAAATCGCAGGGCCTCGAATTTTAGAACATATGGTGGATACAGTCCTATATTTTGAA
GGCGAAAGACATCATAATCATCGTATTTTACGAAGTCAAAAAAACCGCTTTGGCTCTACCAATGAAATTGCTATATTTGA
AATGCTACAGGGTGGTTTAAAAGAGGTGCTGAACCCATCTGAGCTATTTTTGCAGGAGCGTTCACAAGGGGCTGCGGGTT
CCACGATTGTTGCCTCGATGGAGGGAACAAGGCCAATACTCGTTGAAATTCAGTCGCTAGTGACGCCTACAAGCTTTAAT
TACCCTAAACGGATGGCAACCGGGGTAGATCAAAATAGAGTGCAATTGCTGATGGCTGTGCTTGAAAAGCGTATGGGTCT
CATGCTACAAGCACAGGATGCATATATTAAAGTTGCTGGTGGTGTGAAATTGGACGAACCTGCTATAGATTTAGCTGTTC
TGACAAGCATCGTGTCTAGCTTTAAGGATCAGGCTGTAAGGGCAACCGATTGCTTTATTGGTGAAGTGGGTCTGACAGGA
GAGGTACGTCGAGTATCCCGTATCGAGCAGCGTGTCATTGAAGCGGCGAAGCTTGGTTTTAAAAAAGTCTTCATTCCTGC
ATCAAATATAGGTGGTTGGGATTTTCCGCAAGGGATTGAAATTGTCGGTGTAGAAACAATAAAGGATGCATTAAATGCCT
GTTTTAGAGAGTTATAG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(350-432)

AAA domain (AAA_25)

(66-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W7RHU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

70.925

99.127

0.703
  radA Streptococcus pneumoniae Rx1

62.939

99.563

0.627
  radA Streptococcus pneumoniae D39

62.939

99.563

0.627
  radA Streptococcus pneumoniae R6

62.939

99.563

0.627
  radA Streptococcus pneumoniae TIGR4

62.939

99.563

0.627
  radA Streptococcus mitis NCTC 12261

62.939

99.563

0.627
  radA Streptococcus mitis SK321

62.939

99.563

0.627

Multiple sequence alignment