Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   A4A60_RS09250 Genome accession   NZ_CP015222
Coordinates   1777188..1778081 (+) Length   297 a.a.
NCBI ID   WP_069837509.1    Uniprot ID   -
Organism   Bacillus subtilis strain HRBS-10TDI13     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1772188..1783081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4A60_RS09230 (A4A60_09240) - 1772885..1774588 (+) 1704 WP_069837508.1 hypothetical protein -
  A4A60_RS09235 (A4A60_09245) ylqH 1774585..1774866 (+) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  A4A60_RS09240 (A4A60_09250) sucC 1775039..1776196 (+) 1158 WP_003244732.1 ADP-forming succinate--CoA ligase subunit beta -
  A4A60_RS09245 (A4A60_09255) sucD 1776225..1777127 (+) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  A4A60_RS09250 (A4A60_09260) dprA 1777188..1778081 (+) 894 WP_069837509.1 DNA-processing protein DprA Machinery gene
  A4A60_RS09255 (A4A60_09265) topA 1778269..1780344 (+) 2076 WP_003245599.1 type I DNA topoisomerase -
  A4A60_RS09260 (A4A60_09270) trmFO 1780420..1781727 (+) 1308 WP_069837510.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  A4A60_RS09265 (A4A60_09275) xerC 1781795..1782709 (+) 915 WP_014479777.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32972.20 Da        Isoelectric Point: 9.8131

>NTDB_id=177648 A4A60_RS09250 WP_069837509.1 1777188..1778081(+) (dprA) [Bacillus subtilis strain HRBS-10TDI13]
MDQAAACLSICRINQLLSPSHLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQLYPKLQRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKDWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=177648 A4A60_RS09250 WP_069837509.1 1777188..1778081(+) (dprA) [Bacillus subtilis strain HRBS-10TDI13]
TTGGATCAGGCCGCTGCCTGCCTATCGATTTGCAGAATCAATCAATTATTATCCCCATCCCATCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCCGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAACTTTATCCAAAGCTCCAGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCCGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGAAACAAGTGGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGATTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAACACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACGAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTATTTGACCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

97.98

100

0.98
  dprA Lactococcus lactis subsp. cremoris KW2

41.697

91.246

0.38

Multiple sequence alignment