Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   Bt4C1_RS19625 Genome accession   NZ_CP015176
Coordinates   3896170..3896604 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis serovar alesti strain BGSC 4C1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3891170..3901604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Bt4C1_RS19610 (Bt4C1_19615) - 3892348..3892905 (+) 558 WP_000062064.1 PadR family transcriptional regulator -
  Bt4C1_RS19615 (Bt4C1_19620) metE 3893418..3895706 (+) 2289 WP_001007602.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  Bt4C1_RS19620 (Bt4C1_19625) - 3895744..3896133 (-) 390 WP_000424022.1 competence protein ComJ -
  Bt4C1_RS19625 (Bt4C1_19630) nucA/comI 3896170..3896604 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  Bt4C1_RS19630 (Bt4C1_19635) - 3896833..3897600 (+) 768 WP_063675643.1 DUF3967 domain-containing protein -
  Bt4C1_RS19635 (Bt4C1_19640) kinB 3897639..3898913 (-) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  Bt4C1_RS19640 (Bt4C1_19645) - 3899224..3899424 (+) 201 WP_000929277.1 hypothetical protein -
  Bt4C1_RS19645 (Bt4C1_19650) - 3899546..3900709 (+) 1164 WP_000434585.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=177319 Bt4C1_RS19625 WP_000811494.1 3896170..3896604(-) (nucA/comI) [Bacillus thuringiensis serovar alesti strain BGSC 4C1]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=177319 Bt4C1_RS19625 WP_000811494.1 3896170..3896604(-) (nucA/comI) [Bacillus thuringiensis serovar alesti strain BGSC 4C1]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCTTCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment