Detailed information NTG0177300

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: BE966_RS18815
• Genome accession: NZ_CP018970
• Coordinates: 3334497..3335489 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain Ecol_542
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 3329497..3340489

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BE966_RS18785 (BE966_19285)	ispD	3329558..3330268 (+)	711	WP_000246149.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
BE966_RS18790 (BE966_19290)	ispF	3330268..3330747 (+)	480	WP_001219237.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
BE966_RS18795 (BE966_19295)	truD	3330744..3331793 (+)	1050	WP_000568920.1	tRNA pseudouridine(13) synthase TruD	-
BE966_RS18800 (BE966_19300)	surE	3331774..3332535 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
BE966_RS18805 (BE966_19305)	pcm	3332529..3333155 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
BE966_RS18810 (BE966_19310)	nlpD	3333340..3334434 (+)	1095	WP_306278776.1	murein hydrolase activator NlpD	-
BE966_RS18815 (BE966_19315)	rpoS	3334497..3335489 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
BE966_RS18820 (BE966_19320)	-	3335660..3336211 (-)	552	WP_001179444.1	flavodoxin family protein	-
BE966_RS18825 (BE966_19325)	-	3336436..3337479 (+)	1044	WP_001161583.1	MBL fold metallo-hydrolase	-
BE966_RS18830 (BE966_19330)	ygbN	3337622..3338986 (-)	1365	WP_000104433.1	GntP family transporter	-
BE966_RS18835 (BE966_19335)	ygbM	3339075..3339851 (-)	777	WP_001136914.1	HPr family phosphocarrier protein	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=177300 BE966_RS18815 WP_000081550.1 3334497..3335489(+) (rpoS) [Escherichia coli strain Ecol_542]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=177300 BE966_RS18815 WP_000081550.1 3334497..3335489(+) (rpoS) [Escherichia coli strain Ecol_542]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCATTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715