Detailed information NTG0177216

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: BE959_RS14135
• Genome accession: NZ_CP018965
• Coordinates: 2521596..2522588 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain Ecol_517
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2516596..2527588

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BE959_RS14105 (BE959_14470)	ispD	2516657..2517367 (+)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
BE959_RS14110 (BE959_14475)	ispF	2517367..2517846 (+)	480	WP_001374730.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
BE959_RS14115 (BE959_14480)	truD	2517843..2518892 (+)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
BE959_RS14120 (BE959_14485)	surE	2518873..2519634 (+)	762	WP_001374723.1	5'/3'-nucleotidase SurE	-
BE959_RS14125 (BE959_14490)	pcm	2519628..2520254 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
BE959_RS14130 (BE959_14495)	nlpD	2520439..2521533 (+)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
BE959_RS14135 (BE959_14500)	rpoS	2521596..2522588 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
BE959_RS14140 (BE959_14505)	ygbN	2522682..2524046 (-)	1365	WP_000104456.1	GntP family transporter	-
BE959_RS14145 (BE959_14510)	ygbM	2524135..2524911 (-)	777	WP_001136918.1	HPr family phosphocarrier protein	-
BE959_RS14150 (BE959_14515)	ygbL	2524916..2525554 (-)	639	WP_001278994.1	aldolase	-
BE959_RS14155 (BE959_14520)	otnK	2525551..2526813 (-)	1263	WP_000590392.1	3-oxo-tetronate kinase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=177216 BE959_RS14135 WP_000081550.1 2521596..2522588(+) (rpoS) [Escherichia coli strain Ecol_517]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=177216 BE959_RS14135 WP_000081550.1 2521596..2522588(+) (rpoS) [Escherichia coli strain Ecol_517]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715