Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   IEC338SC_RS04830 Genome accession   NZ_CP015145
Coordinates   1014580..1015380 (+) Length   266 a.a.
NCBI ID   WP_212823832.1    Uniprot ID   -
Organism   Acinetobacter pittii strain IEC338SC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1009580..1020380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEC338SC_RS04805 (IEC338SC_0967) - 1010496..1011785 (+) 1290 WP_082188435.1 glycosyltransferase family 4 protein -
  IEC338SC_RS04810 (IEC338SC_0968) - 1011810..1012361 (+) 552 WP_005066176.1 phosphatase PAP2 family protein -
  IEC338SC_RS04815 (IEC338SC_0969) iscX 1012461..1012661 (+) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  IEC338SC_RS04820 (IEC338SC_0970) ndk 1012775..1013206 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  IEC338SC_RS04825 (IEC338SC_0971) rlmN 1013330..1014565 (+) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  IEC338SC_RS04830 (IEC338SC_0972) pilF 1014580..1015380 (+) 801 WP_212823832.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IEC338SC_RS04835 (IEC338SC_0973) - 1015371..1016162 (+) 792 WP_063098084.1 helix-turn-helix domain-containing protein -
  IEC338SC_RS04840 (IEC338SC_0974) ispG 1016184..1017299 (+) 1116 WP_033849427.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IEC338SC_RS04845 (IEC338SC_0975) hisS 1017296..1018588 (+) 1293 WP_063098085.1 histidine--tRNA ligase -
  IEC338SC_RS04850 (IEC338SC_0976) - 1018617..1019315 (+) 699 WP_063098086.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30056.30 Da        Isoelectric Point: 9.0417

>NTDB_id=177103 IEC338SC_RS04830 WP_212823832.1 1014580..1015380(+) (pilF) [Acinetobacter pittii strain IEC338SC]
MSIPKLKTVLCMGIAVAFLAGGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=177103 IEC338SC_RS04830 WP_212823832.1 1014580..1015380(+) (pilF) [Acinetobacter pittii strain IEC338SC]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAGGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCAGTAAAGGTACGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

75.769

97.744

0.741

Multiple sequence alignment