Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   A4W93_RS02190 Genome accession   NZ_CP015118
Coordinates   506746..508275 (-) Length   509 a.a.
NCBI ID   WP_085749059.1    Uniprot ID   A0A1W6L3P9
Organism   Piscinibacter gummiphilus strain NS21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501746..513275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W93_RS02170 (A4W93_02115) - 501939..502661 (+) 723 WP_085749055.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  A4W93_RS02175 (A4W93_02120) - 502689..504077 (+) 1389 WP_085749056.1 ATP-binding protein -
  A4W93_RS02180 (A4W93_02125) - 504041..505306 (-) 1266 WP_085749057.1 class I SAM-dependent RNA methyltransferase -
  A4W93_RS02185 (A4W93_02130) - 505347..506663 (-) 1317 WP_157782103.1 GNAT family N-acetyltransferase -
  A4W93_RS02190 (A4W93_02135) rpoN/rpoN1 506746..508275 (-) 1530 WP_085749059.1 RNA polymerase factor sigma-54 Machinery gene
  A4W93_RS02195 (A4W93_02140) lptB 508354..509106 (-) 753 WP_085749060.1 LPS export ABC transporter ATP-binding protein -
  A4W93_RS02200 (A4W93_02145) lptA 509103..509654 (-) 552 WP_157782104.1 lipopolysaccharide transport periplasmic protein LptA -
  A4W93_RS02205 (A4W93_02150) - 509812..510456 (-) 645 WP_085749062.1 thiol:disulfide interchange protein DsbA/DsbL -
  A4W93_RS02210 (A4W93_02155) - 510520..511149 (-) 630 WP_085749063.1 SPOR domain-containing protein -
  A4W93_RS02215 (A4W93_02160) argS 511160..512845 (-) 1686 WP_085749064.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 509 a.a.        Molecular weight: 56497.52 Da        Isoelectric Point: 4.5776

>NTDB_id=176839 A4W93_RS02190 WP_085749059.1 506746..508275(-) (rpoN/rpoN1) [Piscinibacter gummiphilus strain NS21]
MKPSLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLDVEEDSSTPFEPLAERTSAAERTAEREVDRATE
SPASDASDDTAGVDSAEFGTTERDDWENGTERDDFDGIRETPGSGSSSSNDNDDFEPQDRNPVGPSLQDHLRDQLLGMRL
SAEDAAAVHVLIESLNDDGYLDDPLEDIAAQLADNEEDRETLLERLQVALKWLQNMEPLGVGASNLGECLAIQLRAQERC
EAQTIAIIICKQHLELLAKRDMKKLVAATGADEELIKEAQSLIVALEPKPGRRFARAEANIIVPDVIVQKSGRNWKVVLN
PDVMPKLRINDLYAQAIRQTRGAAGSGAGAALSSRLQEARWFMKNIQQRFDTIQRVSQAIVERQKSFFTHGEIAMKPLVL
REIADELGLHESTISRVTTAKYMATMFGTFELKYFFGSSLNTEAGGNASSTAVRALIKQLVAAEDPNKPLSDSQLSRMLE
EQGIQVARRTVAKYREALRIAPANLRKDM

Nucleotide


Download         Length: 1530 bp        

>NTDB_id=176839 A4W93_RS02190 WP_085749059.1 506746..508275(-) (rpoN/rpoN1) [Piscinibacter gummiphilus strain NS21]
ATGAAACCGTCCCTGCAGGTCCGCCTGTCGCAGCACCTGGCGCTGACGCCGCAGCTGCAGCAGTCCATCCGCCTGCTGCA
GCTCTCCACGCTCGAGCTGCACCAGGAAGTCGAGCAGATGCTCGAGCAGAACCCGTTCCTCGACGTCGAGGAGGATTCGA
GCACGCCGTTCGAGCCGCTCGCCGAACGCACGTCCGCCGCCGAACGCACCGCCGAACGCGAGGTGGACCGCGCCACCGAG
TCCCCGGCCTCCGATGCGTCCGACGACACCGCCGGTGTCGACAGCGCCGAGTTCGGCACCACCGAACGGGACGACTGGGA
AAACGGCACCGAACGCGACGACTTCGACGGCATCCGCGAGACCCCGGGCTCCGGCAGCTCGTCGTCGAACGACAACGACG
ACTTCGAGCCGCAGGACCGCAACCCCGTCGGCCCGAGCCTGCAGGACCACCTGCGCGACCAGCTGCTCGGCATGCGCCTG
AGCGCGGAGGACGCGGCGGCCGTGCACGTGCTGATCGAGTCCCTCAACGACGACGGCTACCTCGACGACCCGCTGGAGGA
CATCGCCGCCCAGCTGGCCGACAACGAGGAAGACCGCGAGACGCTGCTCGAGCGGCTGCAGGTCGCGCTGAAGTGGCTGC
AGAACATGGAGCCGCTCGGCGTGGGCGCGAGCAACCTCGGCGAGTGCCTGGCCATCCAGCTGCGCGCGCAGGAGCGCTGC
GAGGCGCAGACCATCGCCATCATCATCTGCAAGCAGCACCTCGAGCTGCTGGCCAAGCGCGACATGAAGAAGCTCGTCGC
GGCCACCGGGGCCGACGAGGAGCTGATCAAGGAGGCGCAGTCGCTCATCGTCGCGCTCGAACCCAAGCCGGGCCGGCGCT
TCGCGCGCGCCGAGGCGAACATCATCGTGCCCGACGTGATCGTGCAGAAGTCCGGCCGCAACTGGAAGGTCGTGCTGAAC
CCGGACGTGATGCCCAAGCTGCGCATCAACGACCTGTACGCCCAGGCCATCCGCCAGACGCGTGGTGCGGCCGGCAGCGG
CGCGGGTGCCGCGCTCAGCTCGCGGCTGCAGGAAGCGCGCTGGTTCATGAAGAACATCCAGCAGCGCTTCGACACCATCC
AGCGGGTGTCGCAGGCCATCGTCGAACGCCAGAAGAGCTTCTTCACGCACGGCGAGATCGCGATGAAGCCGCTCGTGCTG
CGCGAGATCGCCGACGAGCTGGGCCTGCACGAGTCCACCATCTCGCGCGTGACCACCGCGAAGTACATGGCCACGATGTT
CGGTACCTTCGAGCTGAAGTACTTCTTCGGCTCGTCGCTGAACACCGAGGCCGGCGGCAACGCGTCCAGCACCGCGGTGC
GCGCGCTGATCAAGCAGCTGGTGGCCGCGGAAGACCCCAACAAGCCGCTGTCGGACAGCCAGCTGAGCCGCATGCTCGAG
GAGCAGGGCATCCAGGTGGCGCGCCGCACGGTCGCGAAGTACCGCGAGGCGCTGCGCATCGCGCCGGCCAACCTGCGCAA
GGACATGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(352-507)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(145-333)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6L3P9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

54.688

100

0.55

Multiple sequence alignment