Detailed information NTG0176522

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: A2G07_RS03810
• Genome accession: NZ_CP015081
• Coordinates: 739128..741791 (+)
• Length: 887 a.a.
• NCBI ID: WP_027479822.1
• Uniprot ID: -
• Organism: Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain R1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 734128..746791

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A2G07_RS03780 (A2G07_03785)	-	734936..735421 (+)	486	WP_010888603.1	GreA/GreB family elongation factor	-
A2G07_RS03785 (A2G07_03790)	-	735492..735809 (-)	318	WP_063653039.1	winged helix-turn-helix transcriptional regulator	-
A2G07_RS03790 (A2G07_03795)	-	735931..736569 (+)	639	WP_027479823.1	nitroreductase family protein	-
A2G07_RS03795 (A2G07_03800)	-	736778..737572 (-)	795	WP_010888600.1	enoyl-ACP reductase FabI	-
A2G07_RS03800 (A2G07_03805)	pgeF	737666..738427 (+)	762	WP_010888599.1	peptidoglycan editing factor PgeF	-
A2G07_RS03805 (A2G07_03810)	-	738424..738948 (+)	525	WP_010888598.1	YqeG family HAD IIIA-type phosphatase	-
A2G07_RS03810 (A2G07_03815)	pilB	739128..741791 (+)	2664	WP_027479822.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
A2G07_RS03815 (A2G07_03820)	pilT	741854..743116 (+)	1263	WP_010888596.1	type IV pilus twitching motility protein PilT	Machinery gene
A2G07_RS03820 (A2G07_03825)	-	743179..743691 (-)	513	WP_010888595.1	hypothetical protein	-
A2G07_RS03825 (A2G07_03830)	-	743769..744452 (-)	684	WP_028328057.1	ankyrin repeat domain-containing protein	-
A2G07_RS03830 (A2G07_03835)	-	744641..745840 (-)	1200	WP_010888593.1	acetyl-CoA C-acetyltransferase	-
A2G07_RS03835 (A2G07_03840)	-	745915..746472 (-)	558	WP_010888592.1	nucleotidyltransferase family protein	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 97248.06 Da
• Isoelectric Point: 4.8389

>NTDB_id=176522 A2G07_RS03810 WP_027479822.1 739128..741791(+) (pilB) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain R1]
MALSIGDRRLGAILLDQGYLGDNDLQRALERHSEVGGRLADVLIDSGMVGEKRIARAIEEALGIPLVNLLAVQPDPAALR
SIRPQTALNLQAFPFALEGDRLRVALVDPLSSFSIETLEDDSGFDIEPYQALREEVMWAIATHYPELGLEIVVPSGASDA
GRTGGKLGERLITHGYITDAQLQVALDAQQQTGEALGATLISQRAITEDQLYEVLAEQEGTTFLPNPSGFHPGEEVLGSM
LRADALRLLAVPVDETEQGVTVLTSDPRKRPDIDALIGRPVQLMLTRPRDIERLIEQFYPQRGRLGEQLVQEGTLSRDQL
REALQVQAREGKVKPLGEVITELGFASPDEVDSALQKQNVGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPIQNPP
DPKVALMIPEATARRYVVVPVRLQGNSLVVAMKDPRNVFALDDLKLITGKEILPAVMAEKDIIRLIERYFGEKGFEKLNK
ELAERNKTQQSQEADLSVADESAIVQVVDSIIREAALQDASDIHIETTEDAVKVRYRIDGALREQNSFPKGAAQQIMARL
KIMGHLDIAERRVPQDGRVRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPELEQLGFSEYNYARYTEIIERPNGI
FLVTGPTGSGKSFTCFSTLKRIAKPEKNTTTIEDPIEYEVPGIVQSQVNNSTGMTFARALRAFLRQDPDIIFVGEIRDQE
TAKIAVEAALTGHMVLATLHTNDAPGAVTRLEEMGIENFNISAAVMGVLAQRLVRRVCSECKQPTNADPEVLRRLGISER
DIRGANLMRGTGCPRCGGTGYKGRMGIHELMVMDDSLRRTIGAGRPAAEIRDVALGESGLRSLRQDGIEKALQGLTTLEE
VLAVTAS

Nucleotide

• Length: 2664 bp

>NTDB_id=176522 A2G07_RS03810 WP_027479822.1 739128..741791(+) (pilB) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain R1]
TTGGCTCTTTCGATTGGTGACCGCCGCCTGGGCGCGATTTTGCTGGACCAGGGGTATCTGGGCGACAACGACTTGCAGCG
GGCGCTGGAGCGGCACTCCGAGGTGGGGGGCCGCCTCGCTGACGTGCTGATCGACTCCGGCATGGTGGGCGAAAAACGCA
TCGCCCGCGCCATCGAGGAAGCGCTCGGCATTCCGCTGGTCAACCTGCTGGCGGTGCAGCCCGACCCGGCGGCGCTGCGC
TCGATTCGGCCCCAGACGGCCCTCAACTTGCAGGCGTTTCCCTTCGCGCTCGAAGGTGACCGGCTGCGGGTGGCGCTGGT
GGACCCGCTGTCGAGCTTTTCGATCGAGACGCTTGAAGACGACAGCGGCTTCGACATCGAACCGTATCAGGCGCTGCGCG
AGGAAGTGATGTGGGCCATCGCCACGCACTACCCCGAGCTTGGCCTGGAGATCGTGGTGCCCAGCGGGGCCAGCGACGCC
GGGCGCACCGGAGGCAAGCTCGGTGAGCGGCTGATCACGCACGGCTACATCACCGACGCACAGCTTCAGGTGGCGCTCGA
CGCGCAGCAGCAGACCGGCGAGGCGCTCGGGGCGACCCTGATCTCGCAGCGGGCCATCACCGAAGACCAGCTCTACGAGG
TGCTCGCCGAACAGGAAGGCACCACCTTCCTTCCCAACCCCAGCGGTTTTCACCCCGGCGAGGAAGTCCTCGGCAGCATG
CTGCGCGCCGACGCCCTGCGCCTGCTCGCGGTGCCGGTGGACGAAACCGAGCAGGGCGTGACGGTCCTGACGAGCGACCC
GCGCAAGCGCCCCGACATCGACGCGCTCATCGGGCGCCCAGTGCAGTTGATGCTCACGCGCCCGCGCGACATCGAGCGCC
TGATTGAGCAGTTCTACCCGCAACGGGGCCGCCTCGGCGAGCAACTCGTGCAGGAAGGCACGCTGTCGCGCGACCAGTTG
CGCGAGGCGCTTCAGGTGCAGGCCCGTGAGGGCAAGGTCAAGCCGCTCGGCGAGGTCATCACCGAACTCGGATTCGCCAG
CCCCGACGAGGTGGATTCGGCGCTGCAAAAGCAGAACGTCGGCGGAGGCCGCCTGGAAGACACCCTGGTGCAGTCAGGCA
AGCTCAGCCCCGAGATGCTCGCCCGCTCGCTCGCCGCGCAGCTCGGCTACGAGTTCCTCGACCCCATCCAGAACCCGCCG
GACCCCAAGGTCGCGCTGATGATTCCCGAGGCCACCGCCCGCCGCTACGTAGTGGTGCCGGTCAGGCTCCAGGGCAACTC
GCTCGTCGTCGCCATGAAAGACCCGCGCAACGTGTTTGCGCTCGACGACCTCAAGCTGATTACCGGCAAAGAAATCCTGC
CCGCCGTGATGGCGGAAAAAGACATCATCCGCCTGATCGAGCGCTACTTCGGGGAAAAGGGCTTCGAGAAGCTCAACAAG
GAACTCGCCGAGCGCAACAAGACCCAGCAGTCGCAGGAAGCCGACCTCTCGGTGGCCGACGAGAGCGCCATCGTGCAGGT
GGTGGACTCGATTATCCGCGAGGCCGCGCTGCAAGACGCCTCGGACATTCACATCGAAACCACCGAGGACGCCGTCAAGG
TGCGCTACCGCATCGACGGTGCGCTGCGCGAGCAGAACTCCTTTCCCAAGGGCGCGGCGCAGCAGATCATGGCCCGCCTC
AAGATCATGGGCCACCTCGACATCGCCGAGCGCCGCGTGCCGCAAGACGGGCGCGTGCGCTTCAAGAAGGGCAGCATCGA
CCTCGACCTGCGTCTCTCGACCCTGCCCACCGTGTACGGCGAGAAAGCCGTCATGCGTCTGCTGCAAAAGGCGAGCAACA
TCCCCGAACTCGAGCAGCTCGGCTTTTCCGAGTACAACTACGCCCGCTACACCGAGATTATCGAGCGGCCCAACGGCATT
TTTCTGGTCACCGGGCCGACGGGGTCGGGCAAGTCGTTCACCTGTTTTTCGACCCTCAAGCGCATCGCCAAGCCCGAGAA
AAACACCACGACCATCGAAGACCCCATCGAGTACGAGGTGCCGGGCATCGTGCAGTCGCAGGTGAACAACTCGACCGGGA
TGACCTTTGCCCGCGCCCTGCGCGCCTTCCTGCGTCAGGACCCCGACATCATCTTCGTGGGCGAAATCCGTGACCAGGAA
ACCGCCAAGATTGCGGTGGAAGCGGCGCTCACCGGCCACATGGTGCTCGCCACTCTGCACACCAACGACGCGCCAGGCGC
CGTGACCCGCCTTGAGGAAATGGGCATCGAGAACTTCAACATCTCGGCGGCCGTGATGGGCGTGCTCGCGCAGCGGCTGG
TGCGCCGGGTGTGCAGCGAGTGCAAGCAGCCCACCAACGCCGACCCCGAGGTGCTCCGGCGGCTCGGCATCAGCGAGCGC
GATATTCGCGGCGCGAATCTGATGCGCGGGACCGGCTGTCCCCGCTGCGGCGGCACCGGCTACAAGGGCCGCATGGGGAT
CCACGAGCTGATGGTGATGGACGACTCGCTGCGCCGCACCATCGGGGCCGGGCGGCCCGCCGCCGAAATCCGCGACGTGG
CGCTGGGCGAAAGCGGCCTGCGGAGCCTGCGCCAGGACGGCATCGAAAAGGCCCTGCAAGGCCTGACCACCCTTGAAGAA
GTGCTGGCCGTCACCGCGAGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (511-776)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-472)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (200-303)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	100	100	1
pilF	Thermus thermophilus HB27	56.229	100	0.565