Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A4F89_RS08405 Genome accession   NZ_CP015016
Coordinates   1679550..1680914 (-) Length   454 a.a.
NCBI ID   WP_071539555.1    Uniprot ID   A0AAC9ITL8
Organism   Polynucleobacter asymbioticus strain MWH-RechtKolB     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1674550..1685914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4F89_RS08390 (A4F89_08330) dapD 1676614..1677441 (+) 828 WP_011903295.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  A4F89_RS08395 (A4F89_08335) dapE 1677452..1678603 (+) 1152 WP_011903296.1 succinyl-diaminopimelate desuccinylase -
  A4F89_RS08400 (A4F89_08340) prmB 1678641..1679537 (+) 897 WP_071539554.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  A4F89_RS08405 (A4F89_08345) radA 1679550..1680914 (-) 1365 WP_071539555.1 DNA repair protein RadA Machinery gene
  A4F89_RS08410 (A4F89_08350) - 1680969..1681478 (-) 510 WP_011903299.1 hypothetical protein -
  A4F89_RS08415 (A4F89_08355) - 1681475..1683316 (-) 1842 WP_048812277.1 potassium transporter Kup -
  A4F89_RS12175 - 1683315..1683476 (+) 162 WP_199920149.1 hypothetical protein -
  A4F89_RS08425 (A4F89_08365) - 1683747..1684118 (-) 372 WP_071539556.1 glycine zipper family protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48261.08 Da        Isoelectric Point: 7.3470

>NTDB_id=176114 A4F89_RS08405 WP_071539555.1 1679550..1680914(-) (radA) [Polynucleobacter asymbioticus strain MWH-RechtKolB]
MAKVKTIYICQSCGGTSAKWQGQCPSCQAWNTLEEGLPETTSNARFQGLAQSLPRQKLSAITAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSSAGMNVLYSSGEESAAQIALRAKRIALDAPQLEVLAEIQLEKLISIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVTNPSAIFLSQHEQMVPGACVLVTQEGSRPLLVEIQALVDTAHVPNPR
RLAVGLEQARLAMLLAVLHRHAGIACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKTKIPGLKVIPVERIDQAIAAAAELN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=176114 A4F89_RS08405 WP_071539555.1 1679550..1680914(-) (radA) [Polynucleobacter asymbioticus strain MWH-RechtKolB]
TTGGCTAAAGTCAAAACGATTTATATCTGCCAGTCCTGTGGTGGCACCTCTGCTAAATGGCAAGGTCAGTGTCCCTCATG
CCAAGCTTGGAACACTCTAGAAGAGGGTTTGCCAGAGACCACTTCGAATGCGCGTTTTCAGGGTTTGGCACAATCACTGC
CAAGGCAAAAATTGTCGGCCATTACTGCGGAAGATTTGCCTAGATTTAGTACTGGAGTAGAAGAGTTTGACCGCGTCTTA
GGTGGCGGATTGGTGCCCGGTGGAGTAGTTCTTTTGGGTGGTGATCCCGGCATTGGTAAATCTACTTTACTGTTGCAGGC
TTTAGCTGAGATGAGTTCTGCTGGTATGAATGTTCTCTACAGCAGCGGTGAAGAATCTGCGGCTCAAATTGCACTTCGTG
CAAAACGGATTGCCTTGGATGCACCTCAATTAGAAGTGTTGGCGGAAATTCAGTTAGAAAAACTCATTTCGATTATGGAT
ACCGTCAAGCCACAAGTGTTAGTGGTCGACTCTATTCAAACCTTGTATTCAGAAGTATTAAGTTCTGCACCGGGATCCGT
TGCTCAGGTCAGAGAGTGCGCAGCGCAATTAACTCGAGCAGCTAAATCGAGTGGAATTTGTGTATTGATGGTTGGACATG
TCACTAAAGATGGACATTTGGCGGGCCCTCGCGTCTTAGAGCACATTGTTGATACCGTCCTTTATTTTGAGGGCGATACT
CACTCTTCTTTCCGCCTAGTTCGCTCTATCAAGAATCGTTTTGGCGCAGTAAATGAGTTGGGCGTTTTCGCGATGACCGA
AAAAGGTTTGCGCGGAGTCACCAATCCTTCGGCTATATTCCTATCACAGCACGAACAGATGGTGCCGGGCGCTTGTGTGT
TGGTTACGCAAGAGGGTAGTAGACCGCTTTTAGTCGAAATTCAGGCTTTAGTAGATACAGCACACGTTCCAAACCCGCGG
CGTTTAGCGGTTGGTCTAGAGCAAGCACGTTTGGCAATGTTGTTAGCAGTTTTACATCGCCATGCTGGCATCGCCTGTTT
TGATCAAGATGTTTTCTTAAATGCGGTAGGTGGAGTCAAAATTTCTGAGCCCGCCGCTGACTTAGCGGTGCTGCTTGCTA
TTCAGTCATCTATTCGTAATCGTGCTTTACCTAAAGAGTTGATTGTTTTTGGTGAGGTAGGTTTGGCTGGAGAGATCCGT
CCTTGCCCGCGCGGGCAAGAGCGCTTAAAAGAGGCTGCTAAGTTGGGTTTTACCGTTGCCATTATTCCAAAAGCAAACAT
GCCCAAGACCAAAATACCTGGATTGAAAGTGATTCCGGTAGAGCGAATTGATCAGGCTATCGCAGCAGCTGCCGAACTTA
ATTAA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(358-450)

AAA domain (AAA_25)

(78-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.336

99.559

0.491
  radA Streptococcus pneumoniae Rx1

46

99.119

0.456
  radA Streptococcus pneumoniae D39

46

99.119

0.456
  radA Streptococcus pneumoniae R6

46

99.119

0.456
  radA Streptococcus pneumoniae TIGR4

46

99.119

0.456
  radA Streptococcus mitis NCTC 12261

46

99.119

0.456
  radA Streptococcus mitis SK321

46

99.119

0.456

Multiple sequence alignment