Detailed information NTG0176016

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: BTV17_RS06650
• Genome accession: NZ_CP018806
• Coordinates: 1430900..1433092 (+)
• Length: 730 a.a.
• NCBI ID: WP_075293720.1
• Uniprot ID: A0A9Q6YYI8
• Organism: Histophilus somni strain USDA-ARS-USMARC-63370
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1425900..1438092

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BTV17_RS06620 (BTV17_06605)	uspA	1426183..1426608 (-)	426	WP_011609276.1	universal stress protein UspA	-
BTV17_RS10330 (BTV17_06610)	-	1426597..1426893 (+)	297	Protein_1296	nucleoside triphosphate pyrophosphohydrolase	-
BTV17_RS06630 (BTV17_06615)	-	1426956..1428185 (-)	1230	WP_075293723.1	MFS transporter	-
BTV17_RS06635 (BTV17_06620)	-	1428175..1428576 (-)	402	WP_075293722.1	carboxymuconolactone decarboxylase family protein	-
BTV17_RS10220	-	1428560..1428814 (-)	255	WP_198351450.1	hypothetical protein	-
BTV17_RS10225	-	1428851..1429090 (-)	240	WP_198351451.1	hypothetical protein	-
BTV17_RS10230	-	1429273..1429572 (-)	300	WP_198351452.1	hypothetical protein	-
BTV17_RS06645 (BTV17_06630)	mazG	1430086..1430895 (+)	810	WP_075293721.1	nucleoside triphosphate pyrophosphohydrolase	-
BTV17_RS06650 (BTV17_06635)	priA	1430900..1433092 (+)	2193	WP_075293720.1	primosomal protein N'	Machinery gene
BTV17_RS06655 (BTV17_06640)	-	1433164..1433838 (+)	675	WP_087437276.1	SPOR domain-containing protein	-
BTV17_RS06660 (BTV17_06645)	mreD	1433864..1434352 (-)	489	WP_011609280.1	rod shape-determining protein MreD	-
BTV17_RS06665 (BTV17_06650)	mreC	1434352..1435407 (-)	1056	WP_075293718.1	rod shape-determining protein MreC	-
BTV17_RS06670 (BTV17_06655)	-	1435489..1436544 (-)	1056	WP_011609282.1	rod shape-determining protein	-

Sequence

Protein

• Length: 730 a.a.
• Molecular weight: 83523.11 Da
• Isoelectric Point: 9.7467

>NTDB_id=176016 BTV17_RS06650 WP_075293720.1 1430900..1433092(+) (priA) [Histophilus somni strain USDA-ARS-USMARC-63370]
MKLARVALAVPLFSFFDYLIPNALSTAVGCRVLVPFGRQKLIGIVTELPEKTEIDISQLKPIISVLDHHSVFTPELWHLL
HWGADYYQFPLGEVLISSLPSKLRRGESAVEKSTFFFQLTEQGQQALDNNKLKRAKKQLEALHYLQNNITEKNNNPFHNQ
VWSTLKSKGYISEQEIHKTSISWQQKLNHQSIVKTNDRLPLNQEQALALSQLTFQSGFICWLLEGVTGSGKTEIYLQFIE
EILQQEKQILVLVSEIGLTPQTVQRFQARFNIEIDVLHSNLNDNQRLKVWQRSKNGESAVIIGTRSALFTPFKQLGLIIV
DEEHDLSFKQQDNWRYHARDLAVVYAKQLDIPIVLGSATPSLESINNVRQGKYRHITLKKRARNTTALRHSVIDLKRQIV
HNGLSETLLTMMKNHLKKGNQVLLFLNRRGFAPVLLCHECGWIVECQHCEKPYTYHQHQRVLRCHHCGTQKPIPMQCVNC
GSTHLVTTGLGTEQLEGSLNQRFPNYKITRIDRDSTARKGKLEQYLRDIQLGKSQILIGTQMLAKGHHFPNVTLVALLNV
DSALFSTDFRAEERLAQLYVQVAGRAGREEKQGEVVLQTHYPEHPLLVSLLSQGYPYFAQQALTLRNQMGLPPFSAQALF
KAQSRHSQKAEEVLHQIAAYFHNTSSKNLQILGPIQAPFSKKAGQYRWHLLLQHKSKAELQQILHSFQQENFLRLQQVRL
VLDIDPLDLS

Nucleotide

• Length: 2193 bp

>NTDB_id=176016 BTV17_RS06650 WP_075293720.1 1430900..1433092(+) (priA) [Histophilus somni strain USDA-ARS-USMARC-63370]
ATGAAACTTGCACGAGTTGCTCTTGCCGTTCCTCTGTTTAGTTTTTTTGATTATCTTATTCCAAATGCCCTTTCAACCGC
TGTCGGCTGTCGAGTATTAGTCCCTTTCGGACGACAAAAATTAATTGGTATTGTCACTGAGTTACCTGAAAAAACGGAGA
TAGATATTTCACAACTCAAACCCATTATCTCCGTTCTTGATCATCATTCTGTTTTTACCCCTGAATTATGGCATCTACTA
CATTGGGGAGCGGACTATTATCAATTTCCCTTAGGAGAAGTATTAATAAGCAGCTTGCCAAGCAAATTACGCCGTGGTGA
AAGTGCGGTAGAAAAATCAACATTTTTCTTTCAACTAACGGAACAGGGGCAACAAGCATTAGACAACAACAAATTAAAAA
GAGCAAAAAAACAGCTTGAGGCTCTACATTATCTACAAAACAATATCACAGAAAAAAATAACAATCCTTTTCATAACCAA
GTTTGGAGCACCTTAAAAAGTAAGGGATATATATCTGAACAAGAAATACATAAAACAAGTATCTCTTGGCAACAAAAATT
AAATCATCAATCTATTGTAAAAACTAACGATCGCCTTCCCCTTAACCAAGAGCAAGCTCTTGCACTCAGCCAATTAACCT
TTCAATCCGGATTTATCTGTTGGTTATTAGAAGGCGTAACCGGTTCAGGAAAAACAGAAATCTATCTTCAATTTATTGAG
GAAATTTTACAACAAGAGAAACAAATTTTAGTACTGGTATCGGAAATTGGTCTCACGCCACAAACAGTACAACGCTTTCA
AGCGAGATTTAATATTGAAATTGATGTGTTACATTCAAATTTAAATGATAACCAACGTTTGAAAGTATGGCAACGTTCTA
AAAATGGCGAAAGTGCGGTCATCATCGGCACGAGATCAGCACTATTTACTCCCTTTAAACAACTGGGGCTAATTATCGTT
GATGAAGAACATGATCTTTCATTTAAACAACAAGATAACTGGCGTTATCATGCTCGTGATCTTGCAGTAGTTTACGCTAA
ACAATTGGATATTCCTATCGTGTTAGGATCTGCAACGCCAAGTTTGGAAAGCATCAATAATGTACGACAAGGCAAATATC
GTCACATCACACTCAAAAAAAGAGCAAGAAATACTACCGCACTTCGTCATTCGGTTATCGACCTAAAGCGACAAATAGTA
CATAACGGTTTATCTGAAACCTTGTTGACAATGATGAAAAATCACTTAAAAAAGGGAAATCAGGTTTTATTGTTCTTAAA
CCGCCGTGGTTTCGCACCTGTTCTACTTTGTCATGAATGTGGCTGGATTGTAGAATGCCAACATTGTGAAAAGCCTTATA
CTTATCATCAACATCAGCGAGTTCTACGTTGCCACCATTGCGGAACACAAAAGCCTATTCCAATGCAATGTGTAAATTGC
GGCTCGACACATTTAGTGACGACAGGTTTAGGAACAGAACAATTGGAAGGAAGTTTAAACCAACGTTTTCCAAACTATAA
GATTACCCGTATTGACAGAGATAGTACCGCTCGCAAAGGAAAATTGGAACAATATCTACGGGATATTCAATTAGGCAAAA
GCCAAATTTTAATTGGTACACAAATGCTAGCCAAGGGGCATCATTTCCCCAATGTAACTTTAGTGGCACTGTTAAACGTG
GATAGTGCACTTTTTTCTACTGATTTTCGGGCAGAAGAACGTTTAGCCCAACTTTATGTTCAAGTTGCAGGACGAGCCGG
ACGAGAAGAAAAACAAGGAGAGGTCGTCTTGCAAACCCACTATCCGGAACATCCTTTATTGGTTAGTTTATTAAGTCAAG
GTTATCCTTACTTTGCCCAACAAGCCCTCACTTTGCGTAACCAAATGGGGTTACCGCCTTTCAGTGCTCAAGCACTGTTT
AAAGCCCAAAGTCGCCACTCACAAAAAGCTGAGGAAGTGTTACACCAAATTGCCGCCTATTTTCATAATACCTCAAGCAA
AAACTTGCAAATCTTGGGGCCTATCCAAGCACCTTTTAGCAAAAAAGCGGGGCAATATCGCTGGCACCTTTTATTACAAC
ATAAATCCAAAGCAGAATTACAACAAATTTTACACTCTTTTCAGCAAGAAAATTTTTTAAGATTACAACAAGTGCGGTTA
GTTTTAGATATAGATCCGCTGGATTTGAGCTAG

Domains

Predicted by InterProScan.

• DEAD/DEAH box helicase (DEAD) (222-366)

• Primosomal protein N C-terminal domain (PriA_C) (633-726)

• Helicase conserved C-terminal domain (Helicase_C) (498-588)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-100)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

3D structure

• AlphaFold DB: A0A9Q6YYI8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	42.588	100	0.433