Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AYR56_RS10435 Genome accession   NZ_CP014899
Coordinates   2275220..2276590 (-) Length   456 a.a.
NCBI ID   WP_068223000.1    Uniprot ID   A0A192H266
Organism   Loigolactobacillus backii strain TMW 1.2002     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2270220..2281590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYR56_RS10420 (AYR56_10430) cysS 2270404..2271813 (-) 1410 WP_068281089.1 cysteine--tRNA ligase -
  AYR56_RS10425 (AYR56_10435) gltX 2272380..2273870 (-) 1491 WP_068223005.1 glutamate--tRNA ligase -
  AYR56_RS10430 (AYR56_10440) - 2273994..2275130 (-) 1137 WP_068223002.1 PIN/TRAM domain-containing protein -
  AYR56_RS10435 (AYR56_10445) radA 2275220..2276590 (-) 1371 WP_068223000.1 DNA repair protein RadA Machinery gene
  AYR56_RS10440 (AYR56_10450) - 2276883..2277422 (-) 540 WP_178378338.1 dUTP diphosphatase -
  AYR56_RS10445 (AYR56_10455) - 2277570..2277848 (+) 279 WP_068222997.1 GNAT family N-acetyltransferase -
  AYR56_RS10450 (AYR56_10460) - 2277957..2279294 (+) 1338 WP_068222994.1 aminopeptidase C -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49775.64 Da        Isoelectric Point: 9.2151

>NTDB_id=175068 AYR56_RS10435 WP_068223000.1 2275220..2276590(-) (radA) [Loigolactobacillus backii strain TMW 1.2002]
MTKQKTQYVCQSCGYISPRFLGRCPNCGAWNQMVEELVAAKKPVANAQTNLAGKVSKPELMNQVNIEKEPRVKTKMVELN
RVLGGGIVPGSLVLVGGDPGIGKSTLLLQVSGQLSELGERVLYVSGEESASQIKMRAGRLGVSGQGLYLYPETDMVSIKQ
QINELKPAYVIIDSIQTMQEPELSSVVGSVSQVREVTAELMKLAKQNQITIFIVGHVTKDGSIAGPKILEHMVDTVLYFE
GDRNRTFRILRAVKNRFGSTNEIGIFEMHNGGLHEVKNPSEIFLEERLEGATGSAVVVSLEGTRPILVEIQALVTPTVFG
NAKRTTTGLDHNRAALIMAVLEKRANLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSVASSYRDKETRPTDCFIGEIGLTG
EIRSVNRIEQRIKEAAKLGFKRAFVPRNNMKDVEVPKSIQIITVTTIAEALKKAFS

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=175068 AYR56_RS10435 WP_068223000.1 2275220..2276590(-) (radA) [Loigolactobacillus backii strain TMW 1.2002]
GTGACAAAACAAAAAACACAATATGTTTGCCAGAGCTGTGGTTATATTTCACCACGCTTTTTAGGCCGTTGCCCAAACTG
TGGGGCCTGGAATCAGATGGTTGAAGAGTTAGTTGCGGCTAAAAAACCGGTAGCCAATGCTCAGACTAATTTGGCGGGCA
AGGTGTCTAAGCCGGAATTAATGAATCAAGTTAACATTGAAAAAGAACCACGTGTTAAGACAAAAATGGTTGAATTAAAT
CGAGTACTGGGTGGCGGAATCGTTCCTGGTTCTTTAGTGCTGGTTGGTGGTGATCCGGGGATTGGTAAATCAACCTTGTT
ACTCCAAGTTTCTGGTCAATTAAGTGAGTTAGGCGAGCGAGTGCTTTACGTTTCCGGTGAAGAGAGTGCCTCGCAAATTA
AAATGCGTGCTGGACGACTAGGTGTCTCTGGGCAAGGACTTTATTTATACCCGGAAACAGATATGGTCAGTATCAAACAA
CAGATTAATGAGCTGAAGCCGGCCTACGTCATTATTGATTCAATTCAAACCATGCAAGAGCCGGAATTAAGCTCGGTTGT
GGGCAGTGTTTCGCAAGTTCGTGAAGTAACCGCTGAATTGATGAAATTGGCCAAGCAGAATCAAATTACGATTTTTATTG
TTGGACACGTGACTAAAGATGGGTCAATTGCGGGTCCGAAAATACTGGAACACATGGTTGACACAGTTTTGTACTTTGAA
GGCGATCGGAACCGAACTTTCCGAATTTTACGGGCGGTTAAAAATCGCTTTGGTTCAACTAACGAAATTGGTATTTTTGA
GATGCATAACGGTGGTCTACATGAAGTTAAAAACCCCTCGGAGATCTTTCTTGAGGAACGCTTGGAAGGAGCTACCGGCT
CAGCGGTGGTTGTATCGTTAGAGGGAACGCGGCCGATTCTAGTTGAAATTCAGGCGTTAGTGACACCAACAGTTTTTGGT
AATGCAAAGCGGACTACTACGGGGCTGGACCATAACCGAGCGGCCCTAATTATGGCAGTGCTAGAGAAGCGAGCAAACCT
AATGTTGCAGAATCAGGATGCTTATTTAAAGGCCGCTGGTGGCGTTAAGCTTGATGAGCCGGCAATTGACTTAGCAATTG
CGGTTAGTGTTGCTTCCTCATACCGCGACAAGGAAACGAGACCGACGGATTGTTTTATTGGTGAAATTGGTTTGACTGGG
GAGATTCGGAGCGTTAATCGAATTGAACAGCGAATTAAGGAAGCGGCTAAACTTGGTTTTAAGCGTGCTTTTGTACCACG
CAATAATATGAAGGATGTTGAAGTTCCTAAGTCAATTCAGATTATTACTGTCACGACAATTGCGGAGGCTTTAAAAAAAG
CATTTAGTTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A192H266

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

68.86

100

0.689
  radA Streptococcus pneumoniae D39

68.86

100

0.689
  radA Streptococcus pneumoniae R6

68.86

100

0.689
  radA Streptococcus pneumoniae TIGR4

68.86

100

0.689
  radA Streptococcus mitis NCTC 12261

68.86

100

0.689
  radA Streptococcus mitis SK321

68.64

100

0.686
  radA Bacillus subtilis subsp. subtilis str. 168

63.537

100

0.638

Multiple sequence alignment