Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   BS635_RS12585 Genome accession   NZ_CP018323
Coordinates   2448099..2448539 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain KSC9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2443099..2453539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS635_RS12565 (BS635_12745) coaE 2443589..2444209 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  BS635_RS25870 - 2444234..2444278 (+) 45 WP_120795372.1 protein YacM -
  BS635_RS12570 (BS635_12750) guaC 2444434..2445477 (+) 1044 WP_001217338.1 GMP reductase -
  BS635_RS12575 (BS635_12755) hofC 2445512..2446714 (-) 1203 WP_000157236.1 protein transport protein HofC -
  BS635_RS12580 (BS635_12760) pilB 2446704..2448089 (-) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  BS635_RS12585 (BS635_12765) pilA 2448099..2448539 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  BS635_RS12595 (BS635_12775) nadC 2448742..2449635 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BS635_RS12600 (BS635_12780) ampD 2449723..2450274 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BS635_RS12605 (BS635_12785) ampE 2450271..2451125 (+) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  BS635_RS12610 (BS635_12790) aroP 2451168..2452541 (-) 1374 WP_072133621.1 aromatic amino acid transporter AroP -

Sequence


Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=174956 BS635_RS12585 WP_000360895.1 2448099..2448539(-) (pilA) [Escherichia coli strain KSC9]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=174956 BS635_RS12585 WP_000360895.1 2448099..2448539(-) (pilA) [Escherichia coli strain KSC9]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363


Multiple sequence alignment