Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   PMA3_RS03280 Genome accession   NZ_CP014870
Coordinates   748517..750106 (-) Length   529 a.a.
NCBI ID   WP_064675833.1    Uniprot ID   A0A191YN75
Organism   Pseudomonas silesiensis strain A3     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 743517..755106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMA3_RS03265 (PMA3_03260) - 745473..745874 (+) 402 WP_064675830.1 type IV pilin protein -
  PMA3_RS03270 (PMA3_03265) thiO 745929..747029 (+) 1101 WP_064675831.1 glycine oxidase ThiO -
  PMA3_RS03275 (PMA3_03270) pilR 747174..748520 (-) 1347 WP_064675832.1 sigma-54-dependent transcriptional regulator Regulator
  PMA3_RS03280 (PMA3_03275) pilS 748517..750106 (-) 1590 WP_064675833.1 sensor histidine kinase Regulator
  PMA3_RS03285 (PMA3_03280) - 750096..750329 (-) 234 WP_064675834.1 PP0621 family protein -
  PMA3_RS03290 (PMA3_03285) - 750521..751543 (-) 1023 WP_064675835.1 outer membrane protein assembly factor BamD -
  PMA3_RS03295 (PMA3_03290) rluD 751695..752657 (+) 963 WP_064675836.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PMA3_RS03300 (PMA3_03295) pgeF 752654..753373 (+) 720 WP_064675837.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58501.07 Da        Isoelectric Point: 7.6217

>NTDB_id=174906 PMA3_RS03280 WP_064675833.1 748517..750106(-) (pilS) [Pseudomonas silesiensis strain A3]
MSAETSSHFSNQAQRLLRLYHLYRLSVGITLVLLISSNMDNQLLRFANDDLLRNGSWLYLVLNILLVVFLGNTRRPTQLF
SLALADVLLLCGLFYAGGGVASAIGNLLIVSVAISNTLLQRRIGLLIAAVGALGIVGASLLISFSHPASANDYLQVGTLG
ALCFAAALLVQGLTRRLEVSENLAEQRASEVVGLEALNALILQRMRTGILVLDDQRQVQLANHSALTLLGREALEGNLID
NYSPALVERLQLWRNNPTLRPQSVKVDGSGLELQPSFIALDQSPHHQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLQESEELNGSDRRLTQIIQDHSQRMNRVIENVLQLSRRQQTVPQRLDLKSWLERFVAETREGATAR
QQIHLHIGSGDFKTLMDPNQLTQVVDNLVRNGWRHSIQLHDQAQVWLELFVDRASQLPILEVRDDGTGVAPDHQAKLFEP
FFTTSTQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=174906 PMA3_RS03280 WP_064675833.1 748517..750106(-) (pilS) [Pseudomonas silesiensis strain A3]
GTGAGCGCTGAGACGTCCAGTCACTTCAGCAATCAGGCCCAGCGGCTGCTGCGGCTCTACCATCTTTATCGTTTAAGCGT
CGGCATCACCCTGGTGCTGTTGATCTCCAGCAACATGGACAACCAGTTGCTCAGATTCGCCAATGACGATCTGCTGCGCA
ATGGCAGCTGGCTGTACCTGGTACTCAATATTCTGCTGGTGGTATTTCTAGGGAATACCCGGCGCCCTACGCAATTATTC
AGTCTGGCCCTGGCCGACGTGCTGCTACTGTGCGGCCTGTTCTACGCCGGCGGCGGCGTAGCCAGCGCCATCGGCAACCT
GCTGATCGTGTCGGTGGCGATCAGCAACACTTTGTTGCAACGGCGTATCGGCTTGCTGATTGCCGCTGTCGGCGCGCTCG
GCATCGTCGGGGCGAGCCTGCTGATAAGCTTCAGCCATCCGGCCAGCGCCAACGACTACCTGCAAGTCGGTACGCTGGGC
GCCCTGTGCTTCGCCGCAGCACTGTTGGTACAAGGCCTGACCCGGCGCTTGGAAGTCAGTGAAAACCTGGCCGAGCAACG
GGCCAGCGAAGTGGTCGGCCTCGAAGCCCTCAATGCGCTGATCCTGCAACGCATGCGCACCGGCATCCTGGTGCTCGACG
ACCAGCGACAGGTCCAACTGGCCAACCACAGCGCGTTGACACTGCTGGGTCGGGAAGCACTTGAAGGCAATCTTATCGAT
AATTACTCGCCCGCCCTGGTCGAACGCCTGCAACTGTGGCGCAACAATCCGACGCTGCGCCCCCAGAGCGTCAAAGTCGA
CGGTAGTGGCCTGGAACTGCAACCGAGCTTTATTGCCCTGGATCAAAGCCCTCACCACCAGACTCTGGTATTCCTCGAAG
ACCTCGCCCAGATCGCCCAACAGGCCCAACAATTGAAACTTGCCGCCCTCGGCCGCCTGACAGCCGGCATCGCCCATGAA
ATCCGCAATCCACTGGGCGCCATCAGTCACGCCGCGCAGCTATTACAGGAATCAGAGGAACTCAATGGCTCGGATCGACG
TCTGACCCAGATTATTCAAGACCACTCTCAGCGAATGAATCGGGTAATCGAAAACGTCCTGCAACTGTCCCGTCGGCAAC
AGACCGTGCCGCAACGGCTCGACCTGAAGTCTTGGCTGGAACGGTTCGTCGCCGAAACCCGAGAGGGCGCGACCGCGCGC
CAGCAGATTCACCTGCACATAGGTTCGGGCGACTTCAAAACCCTGATGGATCCGAACCAGCTGACCCAGGTAGTCGACAA
TCTGGTGCGCAACGGCTGGCGCCATAGCATTCAGCTTCACGACCAGGCGCAGGTCTGGCTCGAGCTGTTTGTCGACCGCG
CCAGCCAGCTTCCGATTCTCGAGGTCCGGGACGATGGAACCGGTGTGGCACCGGATCATCAGGCAAAGCTGTTCGAACCG
TTCTTCACCACCAGCACCCAAGGGACGGGGCTTGGACTTTATCTGTCCCGTGAGCTGTGCGAAAGCAATCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGCTGCTTTCGTATCACCTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191YN75

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60

100

0.601

Multiple sequence alignment