Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BS065_RS05845 Genome accession   NZ_CP018256
Coordinates   1253589..1255346 (+) Length   585 a.a.
NCBI ID   WP_001020922.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain AF-673     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1248589..1260346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS065_RS05825 (BS065_05820) - 1249487..1250356 (+) 870 WP_000117606.1 PhzF family phenazine biosynthesis protein -
  BS065_RS05830 (BS065_05825) - 1250411..1251190 (-) 780 WP_000718711.1 ferredoxin--NADP reductase -
  BS065_RS05835 (BS065_05830) tsaA 1251295..1252023 (+) 729 WP_002022825.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  BS065_RS05840 (BS065_05835) - 1252033..1253301 (-) 1269 WP_001052956.1 MgtC/SapB family protein -
  BS065_RS05845 (BS065_05840) pilB 1253589..1255346 (+) 1758 WP_001020922.1 GspE/PulE family protein Machinery gene
  BS065_RS05850 (BS065_05845) - 1255428..1255787 (+) 360 WP_000783090.1 NirD/YgiW/YdeI family stress tolerance protein -
  BS065_RS05855 (BS065_05850) - 1255895..1256557 (+) 663 WP_001221447.1 response regulator transcription factor -
  BS065_RS05860 (BS065_05855) - 1256554..1257912 (+) 1359 WP_001257360.1 sensor histidine kinase -
  BS065_RS05865 (BS065_05860) hemP 1257978..1258148 (-) 171 WP_000993415.1 hemin uptake protein HemP -
  BS065_RS05870 (BS065_05865) - 1258285..1259184 (+) 900 WP_001125744.1 NUDIX domain-containing protein -
  BS065_RS05875 (BS065_05870) - 1259206..1259757 (-) 552 WP_000913439.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 585 a.a.        Molecular weight: 66822.52 Da        Isoelectric Point: 6.9223

>NTDB_id=174860 BS065_RS05845 WP_001020922.1 1253589..1255346(+) (pilB) [Acinetobacter baumannii strain AF-673]
MNIHVEIDTHWCLEQLLQEGRITERDKLLVQTTNRQKDQLKWHPLQWIAHFNLKDQHHVQAHLSLNRLCQWFADRAQLPL
FVIDPLKADVSALTQVMSQEFAIRNHILAVEVHTDRIVIGTDQPFQTDWLNNLEKSLAPKKIERVFLNPEQLQRYLREYY
QVSRAVNSAQKDAAHDRDNNGVEALLQLGDSQNPDANDQHIVKLVDWILQFAFEQSASDIHMEPRKDNGKVRFRIDGVLH
TIYNMPSNTLTAVISRIKILGRLNVAEKRKPQDGRLKTRTPKGQETELRLSTLPTAFGEKLVMRIFDPDVLVRSFQQLGF
DQSLLQQWQRITQNSHGIILVTGPTGSGKTTTLYSSLKQLATEQVNVCTIEDPIEMLEPSFNQMQVNHAIELGFADGVRA
LMRQDPDIIMIGEIRDQDTANMAIQAALTGHLVLSTLHTNDAPSSLTRLHDLGVQPFLTAATILGVLAQRLVRKLCPNCK
QQTHINEDEWQHLTFDYIMEMPEKVYRAVGCEECRHTGYKGRIGIYEFMPVSLELKHLISSHATLNELRSQTKKESVEPL
RIAGARKVLEGLTTLEEVLRVVPLN

Nucleotide


Download         Length: 1758 bp        

>NTDB_id=174860 BS065_RS05845 WP_001020922.1 1253589..1255346(+) (pilB) [Acinetobacter baumannii strain AF-673]
ATGAATATACATGTAGAAATAGACACACATTGGTGTCTAGAACAACTATTACAAGAAGGCCGTATCACTGAACGAGATAA
GTTACTTGTACAAACCACAAATAGGCAAAAAGATCAGTTGAAATGGCATCCATTACAGTGGATTGCGCATTTTAATTTAA
AAGACCAACATCATGTGCAAGCACATTTAAGTTTAAACCGTCTGTGCCAATGGTTTGCAGATCGGGCGCAATTACCTTTA
TTTGTGATTGATCCTTTAAAGGCGGATGTGAGTGCTTTAACTCAAGTCATGTCGCAAGAGTTTGCGATCCGTAATCATAT
TTTAGCTGTAGAAGTCCACACGGACCGAATTGTGATAGGAACAGACCAGCCTTTTCAAACAGACTGGCTAAATAATTTAG
AGAAGAGTTTAGCACCCAAAAAAATTGAACGTGTATTTCTCAACCCCGAGCAATTACAACGTTATTTACGAGAATATTAT
CAAGTCAGCCGAGCAGTAAACTCAGCTCAAAAAGATGCGGCTCATGACCGTGATAACAACGGTGTAGAGGCACTTTTACA
ACTTGGTGATAGCCAAAACCCTGATGCCAATGATCAGCATATTGTGAAATTGGTAGACTGGATTTTGCAATTTGCATTTG
AACAGAGCGCCAGTGACATTCACATGGAACCGCGTAAAGACAATGGCAAAGTTCGGTTCCGTATCGATGGTGTTTTACAT
ACCATTTATAACATGCCTTCAAATACCCTGACCGCCGTGATTTCTCGGATTAAAATTTTAGGGCGGCTAAATGTAGCAGA
AAAGCGTAAACCGCAAGATGGTCGATTAAAAACACGTACGCCGAAAGGACAAGAAACCGAACTACGTCTATCTACCTTAC
CAACAGCGTTTGGTGAAAAACTGGTTATGCGTATTTTTGATCCAGATGTGTTAGTACGTTCCTTTCAGCAATTGGGTTTT
GACCAGTCTTTATTACAACAATGGCAGCGTATTACGCAAAATAGTCACGGCATTATTTTAGTTACGGGGCCAACAGGTTC
TGGTAAAACCACAACGCTATATTCTTCTTTAAAGCAGCTTGCAACCGAGCAGGTTAATGTCTGTACCATTGAAGACCCGA
TTGAAATGCTAGAGCCTAGTTTTAACCAAATGCAGGTCAATCATGCAATTGAACTGGGCTTTGCAGATGGTGTGCGTGCG
CTTATGCGACAAGACCCAGACATTATTATGATTGGGGAAATTCGTGACCAAGATACTGCGAATATGGCCATTCAGGCTGC
ACTCACAGGCCACTTGGTATTATCAACTTTACATACCAATGATGCGCCGTCTAGTTTGACCCGTTTACATGATTTAGGTG
TACAACCATTTTTGACTGCTGCAACAATTTTGGGTGTTCTCGCGCAGCGCTTAGTGCGTAAGCTTTGCCCAAACTGCAAA
CAACAAACTCATATTAATGAAGATGAATGGCAGCACCTAACATTTGACTACATTATGGAAATGCCAGAAAAAGTTTATCG
GGCAGTAGGTTGTGAAGAGTGCCGGCATACGGGTTATAAAGGTCGAATCGGCATTTATGAGTTTATGCCAGTTAGTCTTG
AGCTTAAGCATCTTATTAGCAGTCATGCGACATTAAATGAATTAAGAAGCCAAACTAAAAAAGAAAGTGTAGAGCCTTTA
AGAATTGCAGGAGCTCGCAAAGTTCTTGAAGGTTTGACCACCTTGGAAGAGGTATTGCGGGTTGTTCCATTAAATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(209-474)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(63-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.5

100

0.379

Multiple sequence alignment