Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   BFL16_RS26490 Genome accession   NZ_CP018252
Coordinates   5346903..5347571 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 9000     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5341903..5352571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFL16_RS26460 (BFL16_25235) rfaQ 5342081..5343139 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  BFL16_RS26465 (BFL16_25240) waaA 5343552..5344829 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  BFL16_RS26470 (BFL16_25245) coaD 5344837..5345316 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  BFL16_RS26475 (BFL16_25250) mutM 5345355..5346164 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  BFL16_RS26480 (BFL16_25255) rpmG 5346262..5346429 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  BFL16_RS26485 (BFL16_25260) rpmB 5346450..5346686 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  BFL16_RS26490 (BFL16_25265) radC 5346903..5347571 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  BFL16_RS26495 (BFL16_25270) coaBC 5347743..5348963 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  BFL16_RS26500 (BFL16_25275) dut 5348941..5349399 (+) 459 WP_000976070.1 dUTP diphosphatase -
  BFL16_RS26505 (BFL16_25280) slmA 5349506..5350102 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  BFL16_RS26510 (BFL16_25285) pyrE 5350139..5350780 (-) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  BFL16_RS26515 (BFL16_25290) rph 5350846..5351562 (-) 717 WP_001247093.1 ribonuclease PH -
  BFL16_RS26520 (BFL16_25300) yicC 5351689..5352552 (+) 864 WP_000621336.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=174749 BFL16_RS26490 WP_001297375.1 5346903..5347571(-) (radC) [Escherichia coli strain 9000]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=174749 BFL16_RS26490 WP_001297375.1 5346903..5347571(-) (radC) [Escherichia coli strain 9000]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment