Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   B14_RS12165 Genome accession   NZ_CP014842
Coordinates   2338549..2338977 (-) Length   142 a.a.
NCBI ID   WP_003182220.1    Uniprot ID   A0A1Y0YFI7
Organism   Bacillus licheniformis strain SCDB 14     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2333549..2343977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B14_RS12140 (B14_02426) tkt 2334131..2336134 (-) 2004 WP_009328229.1 transketolase -
  B14_RS12145 (B14_02427) - 2336301..2336534 (-) 234 WP_003182231.1 DUF896 domain-containing protein -
  B14_RS12150 (B14_02428) - 2336600..2337247 (-) 648 WP_003182227.1 YneB family resolvase-like protein -
  B14_RS12155 (B14_02429) yneA 2337266..2337577 (-) 312 WP_003182225.1 cell division suppressor protein YneA -
  B14_RS12160 (B14_02430) lexA 2337730..2338353 (+) 624 WP_003182222.1 transcriptional repressor LexA -
  B14_RS12165 (B14_02431) nucA/comI 2338549..2338977 (-) 429 WP_003182220.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  B14_RS12170 (B14_02432) - 2339336..2340175 (-) 840 WP_003182218.1 thymidylate synthase -
  B14_RS12175 (B14_02433) - 2340235..2340996 (-) 762 WP_003182215.1 hypothetical protein -
  B14_RS12180 (B14_02434) - 2341087..2341920 (-) 834 WP_003182213.1 prohibitin family protein -
  B14_RS22715 - 2341913..2342053 (-) 141 WP_003182211.1 hypothetical protein -
  B14_RS12185 (B14_02435) wrbA 2342373..2342984 (-) 612 WP_003182210.1 NAD(P)H:quinone oxidoreductase -
  B14_RS12190 (B14_02436) - 2343185..2343793 (-) 609 WP_080623603.1 flavin reductase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15332.29 Da        Isoelectric Point: 7.8987

>NTDB_id=174664 B14_RS12165 WP_003182220.1 2338549..2338977(-) (nucA/comI) [Bacillus licheniformis strain SCDB 14]
MIKKWAVHLLFSALVLLGLSGGAAYSPQHAEGAARYDDILYFPASRYPETGAHISDAIKAGHSDVCTIERSGADKRRQES
LKGIPTKPGFDRDEWPMAMCEEGGKGASVRYVSSSDNRGAGSWVGNRLSGFADGTRILFIVQ

Nucleotide


Download         Length: 429 bp        

>NTDB_id=174664 B14_RS12165 WP_003182220.1 2338549..2338977(-) (nucA/comI) [Bacillus licheniformis strain SCDB 14]
ATGATCAAAAAATGGGCGGTTCATCTGCTGTTTTCCGCATTGGTACTGCTTGGGCTTTCGGGAGGCGCCGCATATTCTCC
TCAGCATGCCGAAGGTGCTGCAAGGTATGACGACATATTGTATTTTCCGGCATCACGCTATCCCGAAACCGGCGCTCATA
TCAGCGACGCAATCAAAGCGGGGCATTCAGATGTCTGCACGATTGAAAGATCGGGAGCGGATAAGCGCCGCCAGGAATCA
CTGAAGGGGATTCCGACTAAGCCGGGCTTTGACCGTGACGAATGGCCGATGGCCATGTGTGAAGAAGGGGGCAAAGGAGC
GTCTGTCAGATATGTCAGCTCATCGGATAACCGCGGAGCCGGCTCCTGGGTCGGGAACAGGCTGAGCGGTTTCGCCGACG
GGACGAGAATTTTGTTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0YFI7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.73

88.732

0.521

Multiple sequence alignment