Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   B14_RS08545 Genome accession   NZ_CP014842
Coordinates   1667521..1668150 (+) Length   209 a.a.
NCBI ID   WP_003183693.1    Uniprot ID   Q8VQ77
Organism   Bacillus licheniformis strain SCDB 14     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1662521..1673150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B14_RS08510 (B14_01703) aroE 1663163..1663999 (+) 837 WP_003183706.1 shikimate dehydrogenase -
  B14_RS08515 (B14_01704) yhbY 1663993..1664283 (+) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  B14_RS08520 (B14_01705) - 1664302..1664871 (+) 570 WP_003183702.1 nicotinate-nucleotide adenylyltransferase -
  B14_RS08525 (B14_01706) yqeK 1664861..1665424 (+) 564 WP_003183701.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  B14_RS08530 (B14_01707) rsfS 1665438..1665794 (+) 357 WP_003183699.1 ribosome silencing factor -
  B14_RS08535 (B14_01708) - 1665791..1666546 (+) 756 WP_011198132.1 class I SAM-dependent DNA methyltransferase -
  B14_RS08540 (B14_01709) comER 1666616..1667437 (-) 822 WP_003183696.1 late competence protein ComER -
  B14_RS08545 (B14_01710) comEA 1667521..1668150 (+) 630 WP_003183693.1 helix-hairpin-helix domain-containing protein Machinery gene
  B14_RS08550 (B14_01711) - 1668219..1668788 (+) 570 WP_003183690.1 ComE operon protein 2 -
  B14_RS08555 (B14_01712) - 1668792..1671179 (+) 2388 WP_080623586.1 DNA internalization-related competence protein ComEC/Rec2 -
  B14_RS08560 (B14_01713) - 1671204..1671335 (-) 132 WP_003183686.1 YqzM family protein -
  B14_RS08565 (B14_01714) holA 1671565..1672608 (+) 1044 WP_009327866.1 DNA polymerase III subunit delta -
  B14_RS08570 (B14_01715) rpsT 1672670..1672936 (-) 267 WP_003183682.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22468.42 Da        Isoelectric Point: 5.1890

>NTDB_id=174653 B14_RS08545 WP_003183693.1 1667521..1668150(+) (comEA) [Bacillus licheniformis strain SCDB 14]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGLSIPEEASAQTYDKKEEVKREKSAGKEAVIIDLKGAVKNPGV
YQMKEGDRVHDVLKKAGGTEKKADQKQINLAAVLQDGMVVYIPFEGEEAADSFSKAGSRADGASVDIVNINTASSEELQA
IPGIGPSKAEAVIEYREENGPFHTVEDITNVSGIGEKSFERIKSAITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=174653 B14_RS08545 WP_003183693.1 1667521..1668150(+) (comEA) [Bacillus licheniformis strain SCDB 14]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCTGCGGGAGGCGTTGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCCTCTCCATTCCCGAGGAGGCTTCTGCACAGACGTATGACAAGAAGG
AGGAGGTGAAACGGGAAAAAAGCGCGGGAAAAGAGGCGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAAGAGGGAGACAGGGTCCACGATGTATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGATCAAAAGCA
AATTAACCTTGCAGCCGTTTTGCAGGACGGTATGGTGGTTTACATTCCATTTGAGGGGGAAGAGGCTGCTGATTCTTTCT
CGAAAGCGGGTTCAAGGGCAGATGGCGCTTCCGTCGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGGCG
ATTCCCGGCATCGGCCCTTCAAAAGCGGAAGCGGTTATCGAATACCGCGAGGAGAACGGACCGTTTCACACGGTAGAAGA
CATAACAAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTGCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(147-207)

SLBB domain (SLBB)

(69-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VQ77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

51.402

100

0.526

Multiple sequence alignment